A5053801636- Plant tissue culture and regeneration
- Phytochemistry and Biological Activities
- Essential Oils and Antimicrobial Activity
- Natural product bioactivities and synthesis
- Transgenic Plants and Applications
- Phytochemicals and Antioxidant Activities
- Plant Pathogens and Fungal Diseases
- Berry genetics and cultivation research
- Medicinal Plant Extracts Effects
- Sesquiterpenes and Asteraceae Studies
- Botany and Plant Ecology Studies
- Agriculture, Plant Science, Crop Management
- Herbal Medicine Research Studies
- Natural Antidiabetic Agents Studies
- Pomegranate: compositions and health benefits
- Fungal Biology and Applications
- Phytochemical compounds biological activities
- Phytoestrogen effects and research
- Legionella and Acanthamoeba research
- Phytochemistry Medicinal Plant Applications
- Plant-Microbe Interactions and Immunity
- Plant Taxonomy and Phylogenetics
- Botany, Ecology, and Taxonomy Studies
- Heme Oxygenase-1 and Carbon Monoxide
- Plant Reproductive Biology
Poznan University of Medical Sciences
2014-2023
Grochowski Hospital
2014
Passiflora caerulea L., P. alata Curtis and incarnata L. (synonym for edulis Sims), are the most popular representatives of genus in South America. In recent years, a growing attention is paid to biological activity phytochemical profiles crude extracts from various species worldwide. The aim this study was evaluate compare anti-leukemic dry leaves three greenhouse Poland two human acute lymphoblastic leukemia cell lines: CCRF-CEM its multidrug resistant variant. Two systems liquid...
Eryngium planum L. cell and organ cultures were maintained on Murashige Skoog media (MS), supplemented with exogenous hormones of different types various concentrations for high biomass growth. The callus suspension treated increased sucrose concentration and/or elicited by methyl jasmonate the enhancement selected phenolic acids accumulation. Three acids, rosmarinic acid (RA), chlorogenic (CGA) caffeic (CA), detected HPLC-DAD in those cultures. sum their content dry material was found to be...
A protocol for in vitro production of genetically uniform populations the medicinal plant Eryngium planum, rich selected phenolic acids, has been established. Shoot-tips were collected from axenic seedlings and grown on a Murashige Skoog basal medium supplemented with 6-Benzyladenine (BA) Indole-3-acetic acid (IAA). The highest shoot proliferation efficiency (17 shoots per explant) was obtained when 1.0 mg L−1 BA 0.1 added. Proliferating rooted transferred to soil (89 % frequency survival)....
Eryngium maritimum L. is a valuable medicinal species, but since it protected plant, collection from natural populations forbidden. Therefore, establishing an efficient system for micropropagation of this species desirable. To determine the optimal nutritional factors needed shoot multiplication, root development and secondary metabolites accumulation, different media plant growth regulators were tested. The highest regeneration efficiency (over 96 %), with 4.4 shoots per explant was induced...
Abstract In vitro cultures give the opportunity to perform phytochemical studies on protected species without harvesting plant material from natural environment. Shoots of Eryngium alpinum L. were multiplied Murashige and Skoog (MS) medium in various systems, namely solid media two liquid cultures—stationary agitated, as well via regeneration callus. The biomass increments closely correlated with number shoots arising one explant, which was connected supplementation culture studied growth...
Three species from the Eryngium L. genus—E. campestre, E. maritimum, and planum, plants with a rich chemical composition, were selected for phytochemical biological studies. The applied biotechnological methods allowed to obtain biomass of these rare or protected in form multiplied shoots (stationary system) roots cultured liquid medium (agitated system). In extracts raw material obtained under vitro conditions, content phenolic acids flavonoids (HPLC-DAD method) as well total polyphenols...
Micropropagation of Passiflora species and its hybrids may play an important role in the production healthy disease-free plants which can be a source medicinal herbal products, nutritional fruits ornamental flowers. The rapid multiplication elite to obtain pharmacognostic material, containing valuable flavonoid C-glycosides, is possible by usingcontrolled vitro conditions, constituents medium interactions plant growth regulators (1-naphtaleneacetic acid, benzyladenine, gibberellin...
DNA content was estimated by flow cytometry in seedlings and vitro clones of six species: Oenothera paradoxa, Inula verbascifolia ssp. aschersoniana, Rubus chamaemorus, Solidago virgaurea, S. graminifolia Pueraria lobata. With the exception P. lobata, there no difference genome sizes between cultured plants from any species, indicative that they maintain their genetic stability during culture. This confirms usefulness tissue culture for production certified plant material to obtain herbal medicines.
Abstract In vitro shoot culture of four Solidago species, S.virgaurea L., S.canadensis S.gigantea Ait. and S.graminifolia (L.) Salisb. were established from aseptically germinated seedlings. Proliferated axillary shoots multiplied rapidly on Murashige Skoog's medium, supplemented with 9.3 µm kinetin 11.4 indole‐3‐acetic acid. Rooted plantlets transferred to the soil, then grown in field. Aerial parts collected clonal‐propagated species during flowering stage. The essential oils, obtained...
The essential oils obtained by hydrodistillation from the different parts (inflorescence, stalk leaves, rosette leaves and root) as well in vitro shoot culture of Eryngium planum L. were analyzed GC-FID-MS respect to their chemical composition. E. shoots showed yields. part with higher amount was inflorescences, followed shoots, finally roots. vitro-derived rosettes had totally Quantitative differences also found between compounds intact plant organs. main components leaf oil monoterpene...
Eryngium planum L. has been reported as a medicinal plant used in traditional medicine Europe. The tissue cultures may be an alternative source of the biomass rich desired bioactive compounds. purpose this study was to investigate influence biotechnological techniques on selected phenolic acids accumulation agitated shoot E. planum. Qualitative and quantitative analyses those compounds 50% aqueous - methanolic extracts from were conducted by applying HPLC method. Methyl jasmonate (MeJA),...
The effect of the well-characterized callus extract Chaenomeles japonica on viability, morphology, and proliferation normal human skin fibroblasts was investigated. phytochemical analysis performed using ultra-high performance liquid chromatography method. total phenolic, phenolic acid, flavonoid contents were determined spectrophotometrically. antioxidant activity investigated DPPH (1,1-Diphenyl-1-picrylhydrazyl Radical Scavenging), FRAP (Ferric Reducing Antioxidant Power), CUPRAC (CUPric...
The free-living Acanthamoeba sp.causes various diseases.Treatment of them is very difficult and not always effective because encystation, making it highly resistant to antiamoebic drugs.Gram-positive bacteria staphylococcus aureus, Gram-negative Escherichia coli, an yeast candida albicans also exhibit outstanding resistance antimicrobial substances.The search for new natural amoebicidal agents plant origin still current interest.The aim the study was investigate activity extracts obtained...
Saponin composition of the roots Eryngium planum L. was investigated. Triterpene saponins found in E. and also present maritimum were different from those described previously campestre Three primary isolated their tentative identifications, based on electrospray MS/MS fragmentation patterns, subsequently confirmed by 1D 2D NMR analyses. Their structures established as 3-O-β-d-glucopyranosyl-(1 → 2)-β-d-glucuronopyranosyl-21-O-acetyl-22-O-angeloyl-R1-barrigenol (1)...
Abstract An efficient micropropagation protocol for production of genetically uniform clones Eryngium campestre L. was developed. To determine the effect nutritional and hormonal factors on shoot root development bioactive compounds production, three variants media differing in content macro- micronutrients, as well plant growth regulators various types concentrations were tested. The highest regeneration (100%), with over 13 shoots per explant, induced Murashige Skoog (MS) medium 1.0 mg l...
Genetically uniform plant material, derived from Lychnis flos-cuculi propagated in vitro, was used for the isolation of 20-hydroxyecdysone and polypodine B subjected to an evaluation antifungal antiamoebic activity. The activity 80% aqueous methanolic extracts, their fractions, isolated ecdysteroids were studied against pathogenic Acanthamoeba castellani. Additionally, a Microtox® acute toxicity assay performed. It found that fraction root extract exerts most potent amoebicidal at IC50 0.06...
Eryngium planum L. (EP) is as a rare medicinal plant with lot of potentials pharmaceutical crops. The aim our study was to assess the effect subchronic (28-fold) administration 70% ethanol extract EP roots (200 mg/kg, p.o.) on behavioral and cognitive responses in Wistar rats linked acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), beta-secretase (BACE-1) mRNA levels AChE BuChE activities hippocampus frontal cortex. On last day experiment, 30 min after dose or Huperzine A (HU),...
Phenolic acids and flavonoids, important bioactive compounds of polyphenols, play a significant role in plants; their impact, mainly as antioxidants, on human health have been great interest recent years. The genetically uniform shoots Eryngium alpinum L. cultured vitro, developed via axillary buds regenerated from callus tissue, maintained the media supplemented with various plant growth regulators, were subjected to phenolic flavonoids quantitative analysis applying HPLC-DAD technique. In...
In the present study, essential oils obtained by hydrodistillation of organs Eryngium maritimum and E. alpinum were analyzed GC-FID-MS. The dominant constituents assessed in oil germacrene D (45.2%) fruits; hexadecanoic acid (18.5%), menthol (16.8%), menthone (10.9%) roots; 2,3,4-trimethylbenzaldehyde (11.3%) (10.5%) leaves; shoot culture. case alpinum, main components leaf were: β-elemenone (10.3%), germacrone (5.8%), two selinadienes (7.1% 6.7%), 1,8-cineole (5.3%), which not found from...