Fernando Luiz Tobias

ORCID: 0000-0003-3841-480X
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About
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Research Areas
  • Helminth infection and control
  • Vector-Borne Animal Diseases
  • Animal Disease Management and Epidemiology
  • T-cell and Retrovirus Studies
  • Parasite Biology and Host Interactions
  • Herpesvirus Infections and Treatments
  • Parasitic Diseases Research and Treatment
  • Plant and fungal interactions
  • Microbial infections and disease research
  • Nanoparticles: synthesis and applications
  • Parasites and Host Interactions
  • Mollusks and Parasites Studies
  • Viral gastroenteritis research and epidemiology
  • Animal health and immunology
  • Dermatological diseases and infestations
  • Entomopathogenic Microorganisms in Pest Control
  • Insect and Pesticide Research
  • Animal Behavior and Welfare Studies
  • Parasitic Infections and Diagnostics
  • Infectious Diseases and Mycology
  • Forensic Entomology and Diptera Studies
  • Parasitic infections in humans and animals
  • Virology and Viral Diseases
  • Listeria monocytogenes in Food Safety
  • Antifungal resistance and susceptibility

Universidade Vila Velha
2002-2024

State University of Norte Fluminense
2012

Universidade Federal de Santa Maria
1995-2000

National Council for Scientific and Technological Development
1997

The biosynthesis of metallic nanoparticles (NPs) using biological systems such as fungi has evolved to become an important area nanobiotechnology. Herein, we report for the first time extracellular synthesis highly stable silver NPs (AgNPs) nematophagous fungus Duddingtonia flagrans (AC001). fungal cell-free filtrate was analyzed by Bradford method and 3,5-dinitrosalicylic acid assay used synthesize AgNPs in presence a 1 mM AgNO3 solution. They have been characterized UV-Vis spectroscopy,...

10.2147/ijn.s137703 article EN cc-by-nc International Journal of Nanomedicine 2017-08-01

Helminth parasites cause morbidity and mortality in both humans animals. Most anthelmintic drugs used the treatment of parasitic nematode infections act on target proteins or regulate electrical activity neurons muscles. In this way, it can lead to paralysis, starvation, immune attack, expulsion worm. However, current anthelmintics have some limitations that include a limited spectrum across species threat drug resistance, which highlights need for new human veterinary medicine.Present study...

10.2147/ijn.s193679 article EN cc-by-nc International Journal of Nanomedicine 2019-04-01

Soros de 7956 bovinos leiteiros foram testados pela técnica soro-neutralização para determinar a prevalência anticorpos contra o Herpesvírus Bovino Tipo 1 (HVB 1). As amostras provinham 99 municípios do Estado Rio Grande Sul. Os agrupados em nove bacias leiteiras conforme critérios da Cooperativa Central Gaúcha Leite (CCGL). A encontrada foi 18,8%. Dos 91 (91,9%) apresentaram pelo menos um animal positivo e 8 (8,1%) negativos. maioria das apresentou uma semelhante à demonstrada restante...

10.1590/s0103-84781995000300017 article PT cc-by-nc Ciência Rural 1995-01-01

The objective of the present study was to evaluate biological control nematodes in young ewes receiving fungi Duddingtonia flagrans (AC001) and Monacrosporium thaumasium (NF34) orally. subjects were 40 Texel sheep, all female, between 12 24 months age. At beginning experiment, animals received an oral dose ivermectin. After 14 days, separated four homogeneous groups 10 animals: Group 1 3 g/10 kg pellets containing mycelium AC001 body weight twice a week; 2 NF34 two doses ivermectin (0.2...

10.1080/09583157.2020.1869699 article EN Biocontrol Science and Technology 2021-01-07

Ostrich raising around the world have some key factors and farming profit depend largely on information ability of farmers to rear these animals. Non fertilized eggs from ostriches are discharged in reproduction season. Staphylococcus aureus Escherichia coli microorganisms involved animal human diseases. In order optimize use sub products ostrich raising, non four selected birds were utilized for development polyclonal IgY antibodies. The immunized (200ug/animal) with purified recombinant...

10.1590/s1517-83822012000200015 article EN Brazilian Journal of Microbiology 2012-06-01

The objectives of this study were to describe occurrences Rhabditis spp. causing parasitic otitis in dairy cattle Gir breed the state Espírito Santo, southeastern Brazil, and evaluate biological control nematode using nematophagous fungi Duddingtonia flagrans (AC001) Monacrosporium thaumasium (NF34). After detection collection, three groups formed: two that treated, respectively, with fungal isolates; a group, without fungus. treatments as follows: (a) Petri dishes containing culture medium...

10.1590/s1984-29612019020 article EN cc-by Revista Brasileira de Parasitologia Veterinária/Brazilian Journal of Veterinary Parasitology 2019-04-01

Seqüenciamento e análise filogenética de 17 amostras do vírus da diarréia viral bovina (BVDV) isoladas no Brasil identificaram quatro (23,5%) genótipo 1a (BVDV-1a), nove (52,9%) 1b (BVDV tipo 1b) 2 2). As brasileiras BVDV apresentaram-se genotipicamente distintas dos até então identificados na América Norte Europa, sugerindo pertencerem a um novo subgenótipo. A caracterização antigênica dessas por neutralização cruzada revelou reatividade sorológica muito reduzida com cepas vacinais BVDV. O...

10.1590/s0102-09352000000100003 article PT cc-by Arquivo Brasileiro de Medicina Veterinária e Zootecnia 2000-02-01

Bovine herpesvirus 1 (BoHV-1) causes major losses in worldwide livestock, affecting the respiratory and reproductive tracts of bovine. In past decades, number cases Brazil has been gradually increasing. Therefore, it is important to assess distribution infection different regions country. state Espírito Santo (ES) BoHV rate dairy cattle herds unknown. Thus, aim this study was detect neutralizing antibodies against BoHV-1 serum samples from 1,161 non-vaccinated cows 59 23 municipalities...

10.1590/s0034-737x2014000200017 article EN cc-by Revista CERES 2014-04-01

Determinou-se a quantidade de imunoglobulinas(Igs) séricas em 1.530 amostras coletadas 81 vacas, no período 28 dias pré e pós-parto (n=579), suas terneiras, até 210 (n=951) pela técnica turvação pelo sulfato zinco. Os níveis imunoglobulinas das vacas demonstraram queda nas duas semanas pré-parto, indicando uma transferência Igs da corrente circulatdria para glândula mamária. A média às 24 horas vida 76 terneiras foi 11,66mg/ml (desvio padrão 7,4). Foram considerados como animais com falha...

10.1590/s0103-84781997000300012 article PT cc-by-nc Ciência Rural 1997-08-01

Abstract The aim of this study was to evaluate the combined use different chemical (albendazole, ivermectin, glycerine and Vaseline) biological ( Monacrosporium thaumasium ) compounds in control Ancylostoma caninum. Infective larvae A. caninum were obtained from coprocultures positive faeces naturally infected dogs. We used 1% albendazole, 100% glycerine, Vaseline an isolate nematophagous fungus M. (NF34), alone or combinations. Next, experimental test set up with 16 groups microtubes, a...

10.1017/s0022149x20000334 article EN Journal of Helminthology 2020-01-01

ABSTRACT: Bovine fasciolosis is a neglected parasitic zoonosis that causes economic losses to beef production chains due liver condemnation at abattoirs. Despite the characterization of in several areas Brazil, an update on its prevalence states Rio de Janeiro and Espirito Santo lacking. Thus, from 2018 2021 presented here according cattle origin (municipalities both states). In addition, associated impact abbatoir was analyzed through bovines states. The state Espírito (10.4%) significantly...

10.1590/0103-8478cr20220121 article EN cc-by Ciência Rural 2022-07-26

Recently, the production process of a nanoparticle with nematicidal activity from nematophagous fungus was developed. Thus, new strategy to control parasites has been discovered. The aim present study evaluate in vitro silver nanoparticles (AgNP's) biosynthesized Duddingtonia flagrans filtrate on strongylid larvae ruminants. This is first report AgNP's – D. bovine strongylids, which can be an important biotechnological tool for use produced flagrans.

10.1080/09583157.2021.2013772 article EN Biocontrol Science and Technology 2021-12-19

ABSTRACT: Bovine cysticercosis (BCC) is an important disease in Brazil due to its detection abattoirs, resulting economic losses for cattle farmers, and a public health corcern. Besides importance, the knowledge about BCC epidemiology impact remains not fully described several areas this country, requiring more studies. Thus, study focused on establishing prevalence regions municipalities of state Espírito Santo from 2017 2019, associated risk factors, estimating farmers. A set 2,330 cases...

10.1590/0103-8478cr20210745 article EN Ciência Rural 2022-01-01

Duas amostras do vírus da Diarréia Viral Bovina (BVDV) submetidas a múltiplas passagens em cultivo celular e exposição à radiação ultravioleta (UV) cada passagem foram avaliadas como candidatos vacinais. As testadas quanto sua atenuação para bezerros fetos ovinos, reatividade antigênica contra isolados de campo, capacidade induzir proteção fetal ovelhas prenhes. Inoculação intramuscular (IM) dos modificados quatro produziu apenas uma elevação discreta passageira temperatura corporal, seguida...

10.1590/s0100-736x2002000200006 article PT cc-by-nc Pesquisa Veterinária Brasileira 2002-04-01

ABSTRACT: Rabies is a viral encephalitis that affects mammals, including humans. Rapid and effective laboratory diagnosis of the rabies virus critical for public health. This study evaluated operational, technical, financial viability RT-qPCR in replacement inoculation mice diagnosing routine. A total 316 samples mammalian brains were analyzed, 121 positives 195 negatives, previously diagnosed by direct immunofluorescence (DIF) mouse test (MIT). The submitted to duplex TaqMan technique....

10.1590/0103-8478cr20230229 article EN cc-by Ciência Rural 2024-01-01

ABSTRACT: Anatomopathological descriptions of infections caused by saprophytic algae in dogs are rare. This study reported the anatomopathological and microbiological findings suggestive disseminated protothecosis a dog residing state Espírito Santo, Brazil. During necropsy, macroscopic alterations were observed peripheral lymph nodes, heart, kidneys, pancreas, lungs. Microbiological, cytological, histopathological examinations performed. Cytology histopathology revealed piogranulomatous...

10.1590/0103-8478cr20230415 article EN cc-by Ciência Rural 2024-01-01

Alphitobius diaperinus, popularmente conhecido como cascudinho, atualmente é um dos principais problemas da avicultura mundial. Objetivou-se avaliar o potencial do fungo Pochonia chlamydosporia (VC4) no controle biológico de A. diaperinus em condições laboratoriais. Exemplares adultos inseto foram coletados uma granja avícola, sudeste Brasil. Em seguida, foi realizado crescimento P. meio ágar quitina (AQ2%). Após 10 dias, obtida concentração estruturas fúngicas (conídios/clamidósporos)...

10.26605/medvet-v18n3-6846 article PT cc-by-nc-sa Medicina Veterinária (UFRPE) 2024-10-30

The filtrate of the nematophagous fungus Duddingtonia flagrans produces silver nanoparticles (AgNPs) with nematicidal potential. However, there are currently no reports its activity against Toxocara canis eggs. aim this study was to investigate potential ovicidal AgNPs–D. on T. eggs were obtained from dissection uterus adult female nematodes. After biosynthesis AgNPs, two experimental assays (A and B) performed. In assay A, AgNPs evaluated after 15 30 days interaction. B, inhibition...

10.3390/pathogens13121043 article EN cc-by Pathogens 2024-11-27

Sete amostras citopáticas do vírus da Diarréia Viral Bovina (BVDV) isoladas de casos clínicos e sangue bezerros rebanhos com problemas reprodutivos foram analisadas. Todas as caracterizadas possuíam uma mistura citopáticos (cp) não-citopáticos (ncp), que clonados biologicamente, originando populações puras cada biotipo. Os clones cp ncp obtidos caracterizados antigenicamente um painel anticorpos monoclonais (MAbs) quanto à expressão proteína não-estrutural NS3. A análise reconhecimento pelos...

10.1590/s0103-84782000000100021 article PT cc-by-nc Ciência Rural 2000-03-01
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