- Gold and Silver Nanoparticles Synthesis and Applications
- Nanoparticles: synthesis and applications
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Advanced Nanomaterials in Catalysis
- Fuel Cells and Related Materials
- Animal health and immunology
- Nanoparticle-Based Drug Delivery
- Electrocatalysts for Energy Conversion
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Tuberculosis Research and Epidemiology
- SARS-CoV-2 detection and testing
- Monoclonal and Polyclonal Antibodies Research
- Electrochemical Analysis and Applications
- Diphtheria, Corynebacterium, and Tetanus
- Antimicrobial Peptides and Activities
- Electrochemical sensors and biosensors
- Laser-Ablation Synthesis of Nanoparticles
- Viral gastroenteritis research and epidemiology
- Pomegranate: compositions and health benefits
- Nanocluster Synthesis and Applications
- Enzyme-mediated dye degradation
- Gas Sensing Nanomaterials and Sensors
- Mycotoxins in Agriculture and Food
Universidade Federal do Espírito Santo
2016-2025
Departamento de Ciência e Tecnologia
2015-2017
Microelectronica (Romania)
2014
State University of Norte Fluminense
2011
Universidade Federal do Acre
1982
Background: The antimicrobial activity of essential oils has been reported in hundreds studies, however, the great majority these studies attribute to most prevalent compounds without analyzing them independently. Therefore, aim was investigate antibacterial 33 free terpenes commonly found and evaluate cellular ultrastructure verify possible damage membrane. Methods: Screening performed select substances with activity, then minimal inhibitory concentrations, bactericidal 24-h time-kill curve...
Antibody-coated nanoparticles have recently attracted considerable attention, with the focus falling on diagnostics. Nevertheless, controlled antibody bioconjugation remains a challenge. Here, we present two strategies of aim evaluating best approach for coupling antibodies surface nanomaterials in an oriented way. We employed electrostatic interaction (physical adsorption) and covalent conjugation orientation metallic as methods, their influence detection 17β-estradiol was addressed...
In the present work, we report antioxidant, antimicrobial and cytotoxic activities of quercetin-capped gold nanoparticles (AuNPsQct). The synthesis AuNPsQct was confirmed by UV-Vis spectroscopy, FTIR transmission electron microscopy (TEM) analyses. spectrum showed integrity quercetin molecules on nanoparticle surface. TEM images sizes less than 100 nm a slight spherical shape. electrostatic stability zeta potential method. antioxidant activity quercetin, evaluated DPPH, ABTS nitric oxide...
The biosynthesis of metallic nanoparticles (NPs) using biological systems such as fungi has evolved to become an important area nanobiotechnology. Herein, we report for the first time extracellular synthesis highly stable silver NPs (AgNPs) nematophagous fungus Duddingtonia flagrans (AC001). fungal cell-free filtrate was analyzed by Bradford method and 3,5-dinitrosalicylic acid assay used synthesize AgNPs in presence a 1 mM AgNO3 solution. They have been characterized UV-Vis spectroscopy,...
Green synthesis of metallic nanoparticles has become incredibly popular, mainly by minimizing problems environmental contamination and being able to reduce, stabilize potentially functionalize nanomaterials. Such compounds have possible applications in various areas, e.g., pharmaceuticals (drug delivery systems, cosmetics), textile industry (clothing with antimicrobial properties), diagnostic medicine (imaging, high efficiency biosensors), energy (solar panels), bioremediation, among others....
Monodisperse and colloidally stable magnetic iron oxide nanoparticles have been developed for diverse biotechnology applications. Although promising the adsorption of organic molecules, low density sites in these has a significant challenge. In this study, an optimized factorial design with response surface methodology (RSM) was employed to produce small Superparamagnetic Iron Oxide Nanoparticles (SPIONs) stabilized tetraethoxysilane (TEOS). Bovine Serum Albumin (BSA) selected immobilization...
The interest in applying gold nanoparticles is principally based on their monodispersity. Therefore, the synthesis of monodisperse nanocrystals has a key role designing nanomaterials for specific application. However, it can represent cutting edge many laboratories, mainly because requires variety complex techniques properly characterizing metal with expensive and rugged equipment. Herein, we propose an approach to synthesize controlled monodispersity that be adjusted through factorial...
The use of enzymes immobilized on nanomagnetic supports has produced surprising results in catalysis, mainly due to the increase surface area and potential for recovery reuse. However, meticulous control process difficulties reproducibility have made industrial-scale applications unfeasible. Furthermore, role conjugation strategies catalytic activity recycling catalysts is unclear. Therefore, objective this study was compare through physical adsorption (naked) or covalent bonding with...
In this study, we developed a biosensor based on the localized surface plasmon resonance (LSPR) phenomenon of gold nanoparticles (AuNPs) to detect widely used herbicide glyphosate in food samples. To do so, either cysteamine or specific antibody for were conjugated nanoparticles. AuNPs synthesized using sodium citrate reduction method and had their concentration determined via inductively plasma coupled mass spectrometry. Their optical properties analyzed UV-vis spectroscopy, X-ray...
The cell biology discipline constitutes a highly dynamic field whose concepts take long time to be incorporated into the educational system, especially in developing countries. Amongst main obstacles introduction of new students is their general lack identification with most teaching methods. elaborated figures, movies and animations textbooks has given tremendous contribution learning process search for novel methods been central goal education. Some specialized tools, however, are usually...
The aim was to evaluate the ability of egg yolk antibody (IgY) in blocking Staphylococcus aureus growth vitro. Specific IgY produced by immunizing hens with formalin-killed S. (ATCC 33593). against obtained from yolks their eggs a carrageenan solution. identified SDS-PAGE and Western blot its activity tested ELISA. A inhibition assay protein concentration determination were also conducted. ELISA indicated that specific antigen; this confirmed blotting. inhibited at concentrations 1–5 μg/ml...
Textile wastewater is commonly released into water bodies without appropriated treatment, resulting in environmental damages. Processes involving separation and adsorption using nanomagnetic supports have been considered a promising alternative to address this concern. However, challenges concerning the low stability of nanoparticles reproducibility experiments make their large-scale application difficult. In study, we evaluated efficiency methylene blue (MB) removal by Fe
The development of new routes and strategies for nanotechnology applications that only employ green synthesis has inspired investigators to devise natural systems. Among these systems, the gold nanoparticles using plant extracts been actively developed as an alternative, efficient, cost-effective, environmentally safe method producing nanoparticles, this approach is also suitable large-scale synthesis. This study reports reproducible completely nanocrystals were synthesized Virola oleifera...
Staphylococcus aureus is an opportunistic agent that can cause a variety of infections, both hospital and community-acquired. Epigallocatechin gallate (EGCG), flavonoid present in the leaves Camellia sinensis, has different biological activities, including antimicrobial potential. Here we evaluate antibacterial antibiofilm potential EGCG nine clinical strains S. with genetic profile susceptibilities. The minimum inhibitory concentrations (MIC) ranged from 7.81 to 62.5 μg/mL, bactericidal...