A5056796097- Aquaculture Nutrition and Growth
- Growth Hormone and Insulin-like Growth Factors
- Reproductive biology and impacts on aquatic species
- Physiological and biochemical adaptations
- Animal Genetics and Reproduction
- Regulation of Appetite and Obesity
- Hypothalamic control of reproductive hormones
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Fish Ecology and Management Studies
- Aquaculture disease management and microbiota
- Neuropeptides and Animal Physiology
- Thyroid Disorders and Treatments
- Neurobiology and Insect Physiology Research
- Marine Bivalve and Aquaculture Studies
- Adipose Tissue and Metabolism
- Pancreatic function and diabetes
- Marine and coastal plant biology
- Birth, Development, and Health
- Lipid metabolism and disorders
- Reproductive Biology and Fertility
- Cancer, Hypoxia, and Metabolism
- Plant Physiology and Cultivation Studies
- Synthesis and bioactivity of alkaloids
- Genetic and phenotypic traits in livestock
- Cephalopods and Marine Biology
Kitasato University
2011-2021
Nihon University
2006
The University of Tokyo
1993-2003
University of Washington
1993-2000
NOAA National Marine Fisheries Service
1994-1997
NOAA National Marine Fisheries Service Northwest Fisheries Science Center
1994-1997
Japan Fisheries Research and Education Agency
1995-1997
Seattle University
1993
Nitobe Memorial Nakano General Hospital
1993
Abstract We have identified ghrelin from the stomach of rainbow trout. Four isoforms peptide were isolated: C-terminal amidated type trout (rt ghrelin) composed 24 amino acids (GSSFLSPSQKPQVRQGKGKPPRV-amide) is a basic form; des-VRQ-rt ghrelin, which deleted three (V13R14Q15) rt ghrelin; and further two types that retained glycine residue at C terminus, ghrelin-Gly, ghrelin-Gly. The third serine was modified by octanoic acid, decanoic or unsaturated form those fatty acids. In agreement with...
We have examined the interaction of photoperiod and temperature in regulating parr-smolt transformation its endocrine control. Atlantic salmon juveniles were reared at a constant 10°C or ambient (2°C from January to April followed by seasonal increase) under simulated natural day length. At 10°C, an increase length [16 h light 8 darkness (LD 16:8)] February accelerated increases gill Na + -K -ATPase activity, whereas fish did not respond increased Increases activity both photoperiods...
Two distinct gonadotropins (GTHs) have been demonstrated in a number of teleost fishes. Although the physiological roles GTHs extensively studied salmonids, little is known about their biological functions nonsalmonid In this study, to elucidate role GTH-I and GTH-II reproduction, we cloned α-glycoprotein subunit (αGSU) gonadotropin β subunits (Iβ IIβ) red seabream using 5′- 3′-RACE methods used these cDNA probes reveal changes mRNA levels each during sexual maturation both male female...
We recently identified a cDNA encoding three novel fish hypothalamic neuropeptides, having LPXRF-NH 2 from the goldfish brain. In this study, to clarify physiological functions of these LPXRFamide peptides (gfLPXRFa-1, -2, and -3), we analysed localisation hypophysiotrophic activity using sockeye salmon, Oncorhynchus nerka , in which immunoassay systems for several anterior pituitary hormones have been developed. gfLPXRFa-immunoreactive cell bodies were detected nucleus posterioris...
Igf1 and Igf2 stimulate growth development of vertebrates. In mammals, liver-derived endocrine mediates the promoting effects GH during postnatal life, whereas stimulates placental fetal is not regulated by GH. Insulin enhances production mammalian liver directly, increasing hepatocyte sensitivity to We examined regulation igf1 igf2 mRNA levels GH, insulin, cortisol, insulin cortisol on in primary cultured hepatocytes tilapia, a cichlid teleost. increased both concentration-related biphasic...
Glucocorticoids are known to impede somatic growth in a wide range of vertebrates. In order clarify the mechanisms through which they may act an advanced teleost fish, we examined effects cortisol administration on hormone (GH)/insulin-like factor-I (IGF-I)/IGF-binding protein (IGFBP) system tilapia (Oreochromis mossambicus). short-term experiment, fish were injected intraperitoneally with (2 or 10 microg/g), and killed at 2, 4, 8 24 h after injection. longer-term 48 injection (2, 50...
SUMMARY Atlantic salmon (Salmo salar) juveniles were reared under simulated conditions of normal photoperiod (LDN) or short days (LD 9:15) and ambient temperature (AMB: increases in April) an advanced cycle (ADV: February). Under both conditions, the timing increased peak levels gill Na+,K+-ATPase activity not altered by temperature,although rate increase was initially greater ADV. ADV/LD 9:15 resulted that half seen conditions. Plasma growth hormone (GH) threefold late March ADV/LDN,but...
The pond snail Lymnaea stagnalis is capable of learning taste aversion and consolidating this into long-term memory (LTM) that called conditioned (CTA). Previous studies showed some molluscan insulin-related peptides (MIPs) were upregulated in snails exhibiting CTA. We thus hypothesized MIPs play an important role neurons underlying the CTA–LTM consolidation process. To examine hypothesis, we first observed distribution MIP II, a major peptide MIPs, receptor determined amounts their mRNAs...
GH was identified in the sea lamprey, an extant representative of a group most ancient vertebrates, Agnatha. A putative GH-cDNA cloned from pituitary by RT-PCR. The entire coding region comprised open-reading frame 203 amino acids (aa). mature protein also isolated pituitaries, and fractionated gel filtration reverse-phase HPLC. monitored Western blotting with rabbit antiserum against synthetic peptide corresponding to pre-GH sequence (aa 29–45). Sequence analysis purified demonstrated that...
Hagfish, which lack both jaws and vertebrae, are considered the most primitive vertebrate known, living or extinct. Hagfish have long been enigma of evolution not only because their evolutionary position, but also our knowledge on fundamental processes. Key elements reproductive endocrine system in hagfish yet to be elucidated. Here, presence identity a functional glycoprotein hormone (GPH) elucidated from brown Paramyxine atami. The GPH consists two subunits, alpha beta, synthesized...
Two PRL-releasing peptides (PrRP20 and PrRP31) were recently identified from mammalian hypothalamus by an orphan receptor strategy, a C-terminal RF (arginyl-phenylalamyl-) amide peptide (RFa), structurally related to PrRP, was also the brain of Japanese crucian carp (C-RFa) intestine-contracting assay. However, date there have been no reported studies that examined effects RFa in fish. In present study we determined cDNA, primary structure, function homolog PrRP20 chum salmon, Oncorhynchus...
This study characterized the local effects of extracellular osmolality and prolactin (PRL) on branchial ionoregulatory function a euryhaline teleost, Mozambique tilapia ( Oreochromis mossambicus). First, gill filaments were dissected from freshwater (FW)-acclimated incubated in four different osmolalities, 280, 330, 380, 450 mosmol/kg H 2 O. The mRNA expression Na + /K -ATPase α1a (NKA α1a) /Cl − cotransporter (NCC) showed higher with decreasing media while /2Cl 1a (NKCC1a) PRL receptor...