A5107426779- Acute Myeloid Leukemia Research
- Blood disorders and treatments
- Hematopoietic Stem Cell Transplantation
- Erythrocyte Function and Pathophysiology
- Immune Cell Function and Interaction
- Neutropenia and Cancer Infections
- Sirtuins and Resveratrol in Medicine
- Immune cells in cancer
- RNA modifications and cancer
- Platelet Disorders and Treatments
- Immune Response and Inflammation
- Cancer Genomics and Diagnostics
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Calcium signaling and nucleotide metabolism
- Zebrafish Biomedical Research Applications
- PARP inhibition in cancer therapy
- Angiogenesis and VEGF in Cancer
- Autoimmune and Inflammatory Disorders Research
- Cytomegalovirus and herpesvirus research
- Neuroblastoma Research and Treatments
- Lipid metabolism and disorders
- Acute Lymphoblastic Leukemia research
- Autophagy in Disease and Therapy
- Myeloproliferative Neoplasms: Diagnosis and Treatment
Kumamoto University
2019-2025
Institute of Electrical and Electronics Engineers
2024
University Children's Hospital Tübingen
2016-2023
University of Tübingen
2017-2021
Kitano Hospital
2008-2015
Kyoto University
2008-2014
Rare hematopoietic stem and progenitor cell (HSPC) pools outside the bone marrow (BM) contribute to blood production in stress disease but remain ill-defined. Although nonmobilized peripheral (PB) is routinely sampled for clinical management, diagnosis monitoring potential of PB HSPCs untapped, as no healthy HSPC baseline has been reported. Here we comprehensively delineate human extramedullary compartments comparing spleen, PB, mobilized BM using single-cell RNA-sequencing and/or functional...
HAX1 was identified as the gene responsible for autosomal recessive type of severe congenital neutropenia. However, connection between mutations in and defective granulopoiesis this disease has remained unclear, mainly due to lack a useful experimental model disease. In study, we generated induced pluripotent stem cell lines from patient presenting neutropenia with deficiency, analyzed their vitro neutrophil differentiation potential by using novel serum- feeder-free directed culture system....
Mitochondrial tRNA taurine modifications mediated by mitochondrial translation optimization 1 (Mto1) is essential for the protein translation. Mto1 deficiency was shown to induce proteostress in embryonic stem cells. A recent finding that a patient with MTO1 gene mutation showed severe anemia led us hypothesize dysfunctions may result defective erythropoiesis. Hematopoietic-specific conditional knockout (cKO) mice were lethal and niche-independent defect erythroblast proliferation terminal...
Induced pluripotent stem (iPS) cells are of potential value not only for regenerative medicine, but also disease investigation. The present study describes the development a neutrophil differentiation system from human iPS (hiPSCs) and analysis function differentiation. culture used consisted transfer hiPSCs onto OP9 their with vascular endothelial growth factor (VEGF). After 10 days, TRA 1-85(+) CD34(+) VEGF receptor-2 (VEGFR-2)(high) were sorted co-cultured in presence hematopoietic...
Leukemia stem cells (LSCs) share numerous features with healthy hematopoietic (HSCs). G-protein coupled receptor family C group 5 member (GPRC5C) is a regulator of HSC dormancy. However, GPRC5C functionality in acute myeloid leukemia (AML) yet to be determined. Within patient AML cohorts, high levels correlated poorer survival. Ectopic Gprc5c expression increased aggression through the activation NF-κB, which resulted an altered metabolic state intracellular branched-chain amino acids...
Nicotinamide phosphoribosyltransferase (NAMPT) regulates cellular functions through the protein deacetylation activity of nicotinamide adenine dinucleotide (NAD+)-dependent sirtuins (SIRTs). SIRTs regulate histones and none-histone proteins. The role NAMPT/SIRT pathway in regulation maintenance differentiation human-induced pluripotent stem (iPS) cells is not fully elucidated. We evaluated effects specific inhibitors NAMPT or SIRT2 on pluripotency, proliferation, survival, hematopoietic...
Understanding the molecular mechanisms underlying hematopoietic differentiation of embryonic stem (ES) cells may help to ascertain conditions for in vitro generation cells. Previously, we found that patients with congenital amegakaryocytic thrombocytopenia (CAMT), who develop pancytopenia early after birth, harbor mutations within thrombopoietin (TPO) receptor, c-MPL. This knowledge, together observations and vivo, suggests TPO/c-MPL signaling promotes hematopoiesis. However, TPO are not...
Abstract Genetic mutations in the isocitrate dehydrogenase ( IDH ) gene that result a pathological enzymatic activity to produce oncometabolite have been detected acute myeloid leukemia (AML) patients. While specific inhibitors target mutant enzymes and normalize intracellular level developed, refractoriness resistance has reported. Since acquisition of is accompanied by abrogation crucial WT cells, aberrant metabolism cells can potentially persist even after normalization level. Comparisons...
Familial hemophagocytic lymphohistiocytosis (FHL), which typically has its onset during infancy, is uniformly fatal if not treated. It therefore requires prompt therapeutic intervention. Although hyperferritinemia been emphasized as a useful marker for FHL, some nonfatal cases in infants with spontaneous remission also manifest hyperferritinemia. However, distinguishing them difficult because initial clinical features of these are similar. The authors encountered 14 (serum ferritin >674...
Abstract A 9‐year‐old boy undergoing chemotherapy for conventional osteosarcoma complained of severe abdominal pain associated with rebound tenderness and muscular defense. Abdominal computed tomography indicated intraperitoneal free air. On surgical investigation, a diverticulum‐like lesion, perforated at the base, was found on sidewall ileum. The anatomic location lesion indicative enteric duplication. Although frequency complications is very rare, perforations digestive tract should be...