A5039322799- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Photosynthetic Processes and Mechanisms
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Kruppel-like factors research
- RNA regulation and disease
- Cancer-related gene regulation
- Neurofibromatosis and Schwannoma Cases
- Viral gastroenteritis research and epidemiology
- Sarcoma Diagnosis and Treatment
- Enzyme Structure and Function
- Protein Kinase Regulation and GTPase Signaling
- Mitochondrial Function and Pathology
- Soft tissue tumor case studies
- Biochemical and Molecular Research
- Prion Diseases and Protein Misfolding
- Sepsis Diagnosis and Treatment
- Cell death mechanisms and regulation
- Hippo pathway signaling and YAP/TAZ
- Erythrocyte Function and Pathophysiology
- RNA Research and Splicing
- RNA modifications and cancer
- Plant Stress Responses and Tolerance
- Insect Resistance and Genetics
Chongqing Medical University
2020-2024
Hunan University of Arts and Science
2018
University of Birmingham
1993-2007
University of West London
2005-2006
University of Bedfordshire
2001
Baylor College of Medicine
1996
The Francis Crick Institute
1993
It has been reported that Arabidopsis chloroplast accD transcripts undergo RNA editing and loss of accD-C794 does not affect plant growth under normal conditions. To date, the exact biological role remained elusive. Here, we reveal an unexpected for in response to heat stress. Loss results a yellow dwarf phenotype with decreased gene expression stress, artificial improvement C794-edited enhances tolerance Arabidopsis. These data suggest confers planta. We also found treatment product acetyl...
Juvenile hormone (JH) acid methyltransferase (JHAMT) is a rate-limiting enzyme that converts JH acids or inactive precursors of JHs to active at the final step biosynthesis in insects and thus presents an excellent target for development insect growth regulators insecticides. However, three-dimensional properties catalytic mechanism this are not known. Herein, we report crystal structure JHAMT apoenzyme, holoprotein binary complex with its cofactor S-adenosyl-l-homocysteine, ternary...
The C-terminal binding protein (CtBP) has previously been shown to bind a highly conserved six-amino acid motif very close the C terminus of adenovirus early region 1A (Ad E1A) proteins. We have developed an enzyme-linked immunosorbent assay that facilitated screening synthetic peptides identical or similar site on Ad E1A for their ability CtBP and thus inhibit its interaction with Ad12 E1A. It amino acids both N-terminal original proposed contribute CtBP. Single substitutions across...
OBJECTIVES: Sepsis remains a highly lethal disease, whereas the precise reasons for death remain poorly understood. Prokineticin2 is secreted protein that regulates diverse biological processes. Whether prokineticin2 beneficial or deleterious to sepsis and underlying mechanisms unknown. DESIGN: Prospective randomized animal investigation in vitro studies. SETTING: Research laboratory at medical university hospital. SUBJECTS: deficiency wild-type C57BL/6 mice were used vivo studies; patients...
Although recent research progress on the abundant C-to-U RNA editing events in plant chloroplasts and mitochondria has uncovered many recognition factors their molecular mechanisms, intrinsic regulation of within plants remains largely unknown. This study aimed to establish a regulatory relationship Arabidopsis between hormone auxin chloroplast editing. We first analyzed response elements (AuxREs) present promoters reported date. found that each more than one AuxRE, suggesting potential role...
An analogue of elongation factor Tu (EF‐Tu) from Escherichia coli was prepared by biosynthetic incorporation 3‐fluorotyrosine. The 19 F‐NMR spectra the binary complexes this protein with GDP, GTP and Ts (EF‐Ts) ternary EF‐Tu · GDP aurodox EF‐Ts were measured. contains ten tyrosine residues all studied gave complex F overlapping resonances. a spectrum in which two signals markedly different those shown other complexes, resonances being shifted downfield at least 3.4 ppm 0.9 relative to their...
Abstract Background Malignant ‘triton’ tumor is an extremely rare subtype of malignant periphery nerve sheath tumors. Clinical diagnosis triton difficult before surgery due to its low incidence and the lack knowledge. Therefore, describe summarize CT imaging characteristics great assistance for early preoperative diagnosis. Case presentation Two cases suspected MTT by scan operation were closely observed. The was eventually confirmed immunochemical assay, which verified speculation scans....
Previous detailed mutational analysis has shown that the binding site on adenovirus (Ad) early region 1A (E1A) for TATA-binding protein (TBP) is located toward N terminus of conserved 3 (CR3). Here we demonstrate synthetic peptides between 15 and 22 amino acids, identical to acid sequences CR3 present in larger Ad5 E1A (13 S product) both Ad12 12 products) proteins lie N-terminal zinc finger motif, can disrupt TBP. These findings suggest are biologically active terms interacting with TBP...
The adenovirus C5 E1B-55K protein is crucial for viral replication and expressed early during infection. It can interact with E4orf6 to form a complex that functions as ubiquitin E3 ligase. This targets specific cellular proteins marks them ubiquitination and, predominantly, subsequent proteasomal degradation. interacts various proteins, p53 being the most extensively studied, although identifying binding sites has been challenging. To explain diverse range of associated E1B-55K, we...
Osteoporosis (OP) is a debilitating skeletal abnormality involving bone remodeling and cell homeostasis characterized by decreased strength high fracture risk. A novel therapeutic intervention for OP manipulating cellular autophagy–apoptosis processes to promote presented. Protective effects of the naturally occurring plant extract Liquiritigenin (LG) were demonstrated in an ovariectomy (OVX)-OP mouse model preosteoblast MC3T3-E1 cells. Micro-CT histological staining assessments phenotype...
This research analyzed small peptides equivalent to a region within the human adenovirus early 1A protein that confers, in part, tumor-inducing properties various degrees on several viral strains rats and mice. The oncogenic spacer is α-helical, which contrasts with previous suggestions this unstructured. helix characterized by stretch of amino acids rich alanine residues are organized into hydrophobic, or “water-hating,” surface considered form major site interaction cellular targets...
Abstract Background: malignant triton tumors (MTTs) are an extremely rare subtype of periphery nerve sheave (MPNSTs). Clinical diagnosis MTTs is difficult before surgery due to its low incidence and the lack knowledge. Therefore, describe summarize computed tomography (CT) imaging characteristics great assistance for early preoperative diagnosis. Case presentation: Two case reports were closely observed in our hospital, with presumptive MTT by CT scan examination surgery. The was eventually...
Using fluorescence spectroscopy we have identified a binding region for Ras on the GTPase activating protein (GAP) lying within residues 715–753. A synthetic peptide Y922, corresponding to 716–753 of GAP binds wild type showing 3.3‐fold higher affinity GTP‐ over GDP‐bound forms Ras. Binding is stabilised by Mg 2+ , although Y922 does not stimulate activity Peptide Y32A and Y40F mutants showed equal both GDP‐ GTP‐bound forms, with · GDP abolished in absence . These results suggest that mimics...