A5059950231- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Neurogenesis and neuroplasticity mechanisms
- Down syndrome and intellectual disability research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Mitochondrial Function and Pathology
- Nerve injury and regeneration
- Ovarian function and disorders
- Grief, Bereavement, and Mental Health
- Natural product bioactivities and synthesis
- Peroxisome Proliferator-Activated Receptors
- Cerebral Palsy and Movement Disorders
- Adipose Tissue and Metabolism
- Phytochemistry and Biological Activities
- Maternal Mental Health During Pregnancy and Postpartum
- Reproductive Health and Technologies
- Pancreatic function and diabetes
- Birth, Development, and Health
- Maternal and Perinatal Health Interventions
- Acne and Rosacea Treatments and Effects
- Reproductive Health and Contraception
- Genetics and Neurodevelopmental Disorders
- Assisted Reproductive Technology and Twin Pregnancy
- Metabolism and Genetic Disorders
- Urticaria and Related Conditions
Institut d'Investigació Biomédica de Bellvitge
2012-2024
Universitat Autònoma de Barcelona
2020-2024
Universidade de São Paulo
2023
Centre for Biomedical Network Research on Rare Diseases
2012-2017
Instituto de Salud Carlos III
2012-2017
University of Bologna
2006-2016
ASL Roma
2016
National Academies of Sciences, Engineering, and Medicine
2013-2014
National Research Council
2013
Institute of Neurobiology and Molecular Medicine
2012-2013
During spermiogenesis, mammalian chromatin undergoes replacement of nuclear histones by protamines, resulting in a DNA that is highly condensed the mature sperm. We have previously demonstrated percentage human spermatozoa exhibit 1) positivity to guanine-cylosine-specific chromomycin A3 (CMA3) fluorochrome and 2) presence endogenous nicks their DNA. In situ protamination sperm limits positive CMA3 exhibiting nicks. this study, we report further investigations aim clarify relationship...
In this study we investigated whether morphology and chromatin anomalies in human spermatozoa can influence fertilization after intracytoplasmic sperm injection (ICSI). We examined unfertilized oocytes, using the fluorochrome Hoechst 33342, to determine a relationship exists between failure of quality. Sperm packaging quality was assessed chromomydn A3 (CMA3) fluorochrome, presence DNA damage spermatozoa, in-situ nick translation. Normal males present parameters with normal >20%, CMA3...
A major event in enhancing sperm chromatin stability is the replacement of histones by protamines during spermiogenesis. In this study, we present results indicating that chromomycin A3 (CMA3) can be used to show protamine deficiency chromatin. Fixed mature mouse spermatozoa showed high fluorescence after treatment with ethidium bromide (EB), but was completely unstained CMA3. The same found highly resistant situ nick-translation. contrast, a substantial fraction human were positive for...
In-vitro fertilization (IVF) embryos are selected for transfer on the basis of morphology and rate development. However, when a number have similar characteristics, selection best is left to chance. Recently, we proposed simple, novel method overcome this problem, based pre-selection cleaving early two-cell stage. In study adopted same choose fertilized after intracytoplasmic sperm injection (ICSI). Fertilized that had cleaved stage by 27 h post-injection were designated as 'early cleavage'...
Down syndrome (DS) is a genetic pathology characterized by intellectual disability and brain hypotrophy. Widespread neurogenesis impairment characterizes the fetal neonatal DS brain, strongly suggesting that this defect may be major determinant of mental retardation. Our goal was to establish, in mouse model for DS, whether early pharmacotherapy improves cognitive behavior. Neonate Ts65Dn mice were treated from postnatal day (P) 3 P15 with fluoxetine, an antidepressant inhibits serotonin...
We report on 332 infertile couples who underwent 1115 cycles of intrauterine insemination (IUI) with washed husband's semen. The indication for IUI was an abnormal post-coital test due to either a male or cervical infertility factor. mean number per patients 3.4, the overall pregnancy rate 18.7%, and cycle 5.6%. cumulative calculated by life table analysis showed that 16.0% pregnancies occurred in first three treatment cycles, while 26.9% sixth cycle. outcome therapy adversely affected if...
This study aimed to investigate the association between anomalies in sperm chromatin packaging, morphology and fertilization patients undergoing routine in-vitro (IVF) or subzonal insemination (SUZI). Sperm packaging was assessed using chromomycin A3 (CMA3), a fluorochrome specific for guanine-cytosine rich sequences of DNA. One hundred 150 cells were 55 compare after IVF SUZI. When CMA3 fluorescence individual spermatozoa assessed, >75% macrocephalic fluoresced all patients. In contrast...
Mammalian spermiogenesis involves the replacement of histones by protamines, resulting in a highly compacted chromatin. Upon fertilization, reverse process occurs. We have previously shown that chromomycin A3 (CMA3) fluorochrome represents useful tool for detecting protamine deficiency spermatozoa. In this study we investigated CMA3 accessibility and presence endogenous nicks maturing fertilizing mouse sperm. Testicular sperm stages 1–7 8–14 showed high positivity (> 96% ) to CMA3,...
A common process associated with oxidative stress and severe mitochondrial impairment is the opening of permeability transition pore, as described in many neurodegenerative diseases. Thus, inhibition pore represents a potential target for inhibiting mitochondrial-driven cell death. Among components, cyclophilin D most studied has been found increased under pathological conditions. Here, we have used vitro vivo models X-linked adrenoleukodystrophy to investigate relationship between redox...
Intellectual impairment is a strongly disabling feature of Down's syndrome, genetic disorder high prevalence (1 in 700–1000 live births) caused by trisomy chromosome 21. Accumulating evidence shows that widespread neurogenesis major determinant abnormal brain development and, hence, intellectual disability syndrome. This defect worsened dendritic hypotrophy and connectivity alterations. Most the pharmacotherapies designed to improve cognitive performance syndrome have been attempted mouse...
The peroxisomal disease adrenoleukodystrophy (X-ALD) is caused by loss of the transporter very-long-chain fatty acids (VLCFAs), ABCD1. An excess VLCFAs disrupts essential homeostatic functions crucial for axonal maintenance, including redox metabolism, glycolysis and mitochondrial respiration. As function morphology are intertwined, we set out to investigate role dynamics in X-ALD models. Using quantitative 3D transmission electron microscopy, revealed fragmentation corticospinal axons...
Down syndrome (DS), a high-incidence genetic pathology, involves brain hypoplasia and mental retardation. Emerging evidence suggests that reduced neurogenesis may be major determinant of underdevelopment in DS. To establish whether it is possible to improve DS, Ts65Dn mice--the most widely used model for DS--and euploid mice were treated with control or lithium chow 1 month. During the last 3 days animals received one daily injection 5-bromo-2-deoxyuridine (BrdU)--a marker proliferating...
Abstract Down syndrome DS is a genetic pathology characterized by brain hypotrophy and severe cognitive impairment. Although defective neurogenesis an important determinant of mental disability, dendritic appears to be equally factor. A previous study showed that fluoxetine, selective serotonin reuptake inhibitor, fully restores in the Ts65Dn mouse model . The goal current was establish whether fluoxetine also development. In mice aged 45 days, treated with postnatal period P 3– 15, we...
We aimed to evaluate the nerve growth factor (NGF) pathway and its influence on corneal healing mechanisms in normal conditions an animal model of denervation induced by capsaicin.Peripheral sensory damage was rat pups subcutaneous injection capsaicin effects evaluated hot-plate test, count, tear secretion. Corneal capsaicin-treated -untreated rats epithelial scraping. Healing rate; NGF (NGF, tyrosine kinase A [TrkA], p75); stem cell marker p63 were RT-PCR, ELISA, Western blot,...
Summary Onset of capacity for childbearing in women is dated biologically by menarche, although actual onset may be delayed. The end less understood but recent demographic and biological research on fertility at older ages clarifying the fertility. view declining with age based age-specific natural populations, artificial insemination pregnancy rates World Fertility Survey data. New data from Demographic Health Surveys exposure to risk shows that whereas need longer pregnancy, declines...
A cytogenetic study on 25 breast fibroadenomas from 17 women is reported. Seven tumors in five patients showed clonal structural chromosome changes. In three the breaks involved 12, occurring two band 12p12 and 12q15 all of one patient. The finding an identical aberration, t(11;12)(q21;q15), adenomas same patient strongly suggests a origin multiple breast.
Abstract The proteome of quiescent human platelets was analyzed by a shotgun proteomics approach consisting enzymatic digestion, peptide separation based on isoelectric point the use OFFgel fractionation and, finally, RP nanoscale chromatography coupled to MS/MS detection (nano‐LC‐MS/MS). in first dimension effective providing an additional separation, orthogonal nano‐LC, thus generating off‐line multidimensional platform that proved be robust and easy set up. analysis identified 1373...