A5025743435- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant responses to water stress
- Molecular Biology Techniques and Applications
- Genomics and Phylogenetic Studies
- Food composition and properties
- Plant Disease Resistance and Genetics
- Seed Germination and Physiology
- Microbial Metabolites in Food Biotechnology
- Biofuel production and bioconversion
- Plant Stress Responses and Tolerance
- Wheat and Barley Genetics and Pathology
- Rice Cultivation and Yield Improvement
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Animal Genetics and Reproduction
- Soybean genetics and cultivation
- Legume Nitrogen Fixing Symbiosis
- Genetic and phenotypic traits in livestock
- Plant Pathogens and Resistance
- Plant Reproductive Biology
- Lipid metabolism and biosynthesis
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cell death mechanisms and regulation
- Plant Pathogens and Fungal Diseases
Leibniz Institute of Plant Genetics and Crop Plant Research
2011-2025
Leibniz Association
2014
Leibniz Institute for Neurobiology
2012
Park University
1979
Abstract Nucellar projection (NP) and endosperm transfer cells (ETC) are essential tissues in growing barley (Hordeum vulgare) grains, responsible for nutrient from maternal to filial tissues, endosperm/embryo nutrition, grain development. A laser microdissection pressure catapulting-based transcriptome analysis was established study NP ETC separately using a 12K macroarray. major challenge isolate high-quality mRNA preembedded, fixed tissue while maintaining integrity. We show that probes...
Abstract Barley (Hordeum vulgare) grain development follows a series of defined morphological and physiological stages depends on the supply assimilates (mainly sucrose) from mother plant. Here, spatio-temporal patterns sugar distributions were investigated by mass spectrometric imaging, targeted metabolite analyses, transcript profiling microdissected tissues. Distinct balances observed, which may relate to differentiation filling processes. Notably, various types oligofructans showed...
High-resolution spatial transcriptomics identifies previously unknown regulators for barley floral meristem development.
Abstract Background Seed metabolism is dynamically adjusted to oxygen availability. Processes underlying this auto-regulatory mechanism control the metabolic efficiency under changing environmental conditions/stress and thus, are of relevance for biotechnology. Non-symbiotic hemoglobins have been shown be involved in scavenging nitric oxide (NO) molecules, which play a key role sensing/balancing plants animals. Steady state levels NO suggested act as an integrator energy carbon subsequently,...
Barley endosperm cells differentiate into transfer (ETCs) opposite the nucellar projection. To comprehensively analyse ETC differentiation, laser microdissection-based transcript and metabolite profiles were obtained from microdissected tissues cell morphology was analysed. Flange-like secondary-wall ingrowths appeared between 5 7 days after pollination within three outermost layers. Gene expression analysis indicated that ethylene-signalling pathways initiate morphology. This is accompanied...
Programmed cell death is essential part of development and homeostasis any multicellular organism. We have analyzed programmed in developing barley caryopsis at histological, biochemical molecular level. Caspase-1, -3, -4, -6 -8-like activities increased with aging pericarp coinciding abundance TUNEL positive nuclei expression HvVPE4 HvPhS2 genes the tissue. TUNEL-positive were also detected nucellus nucellar projection as well embryo surrounding region during early development. Quantitative...
Background Cell specification and differentiation in the endosperm of cereals starts at maternal-filial boundary generates transfer cells (ETCs). Besides importance assimilate transfer, ETCs are proposed to play an essential role regulation by affecting development proximate tissues. We attempted identify signalling elements involved early using a combination laser-assisted microdissection 454 transcriptome sequencing. Principal Findings sequencing differentiating ETC region from syncytial...
In cereal grains, the maternal nucellar projection (NP) constitutes link to filial organs, forming a transfer path for assimilates and signals towards endosperm. At transition storage phase, NP of barley (Hordeum vulgare) undergoes dynamic regulated differentiation characteristic pattern proliferating, elongating, disintegrating cells. Immunolocalization revealed that abscisic acid (ABA) is abundant in early non-elongated but not differentiated maternally affected shrunken-endosperm mutant...
Endosperm development in barley starts with the formation of a multinucleate syncytium, followed by cellularization ventral part syncytium generating endosperm transfer cells (ETCs) as first differentiating subdomain, whereas aleurone (AL) will originate from periphery enclosing syncytium. Positional signaling syncytial stage determines cell identity cereal endosperm. Here, we performed morphological analysis and employed laser capture microdissection (LCM)-based RNA-seq ETC region...
Cereal endosperm represents the most important source of world's food; nevertheless, molecular mechanisms underlying cell and tissue differentiation in cereal grains remain poorly understood. Endosperm cellularization commences at maternal-filial intersection generates transfer cells (ETCs), a type with prominent anatomy optimized for efficient nutrient transport. Barley HISTIDINE KINASE1 (HvHK1) was identified as receptor component spatially restricted expression syncytial where ETCs...
Some eukaryotes exhibit dramatic genome size differences between cells of different organs, resulting from programmed elimination chromosomes. Here, we present the first transcriptome analysis chromosome using laser capture microdissection (LCM)-based isolation central meristematic region Aegilops speltoides embryos where B (B) occurs. The comparative RNA-seq with (Bplus) and without Bs (B0) allowed identification 14,578 transcript isoforms (35% out 41,615 analyzed isoforms) that are...