A5035371642- Inflammasome and immune disorders
- interferon and immune responses
- Toxin Mechanisms and Immunotoxins
- Cell death mechanisms and regulation
- Gout, Hyperuricemia, Uric Acid
- Peptidase Inhibition and Analysis
- Anesthesia and Neurotoxicity Research
- Galectins and Cancer Biology
- Kawasaki Disease and Coronary Complications
- Escherichia coli research studies
- Protein Hydrolysis and Bioactive Peptides
- DNA Repair Mechanisms
- IL-33, ST2, and ILC Pathways
- Immune Response and Inflammation
- Autophagy in Disease and Therapy
- Toxoplasma gondii Research Studies
- Salmonella and Campylobacter epidemiology
- Essential Oils and Antimicrobial Activity
- Cancer, Stress, Anesthesia, and Immune Response
- Listeria monocytogenes in Food Safety
- PARP inhibition in cancer therapy
- Lipid Membrane Structure and Behavior
- Antimicrobial Peptides and Activities
- Studies on Chitinases and Chitosanases
- DNA and Nucleic Acid Chemistry
Chinese Academy of Sciences
2020-2024
National Institute of Biological Sciences, Beijing
2020-2024
Institute of Biophysics
2020-2024
Nanjing University of Chinese Medicine
2022-2023
Zhejiang A & F University
2020-2022
Sun Yat-sen University
2020
Beijing University of Chemical Technology
2017
Gasdermins (GSDMs) are pore-forming proteins that execute pyroptosis for immune defense. GSDMs two-domain activated by proteolytic removal of the inhibitory domain. In this work, we report two types cleavage-independent GSDM activation. First, Tricho GSDM, a domain–only protein from basal metazoan Trichoplax adhaerens , is disulfides-linked autoinhibited dimer reduction disulfides. The cryo–electron microscopy (cryo-EM) structure illustrates assembly mechanism 44-mer pore. Second, RCD-1-1...
Blocking host cell death is an important virulence strategy employed by many bacterial pathogens. We recently reported that Shigella flexneri inhibits pyroptosis delivering a type III secretion system (T3SS) effector OspC3 catalyzes novel arginine ADP-riboxanation modification on caspase-4/11. Here, we investigated the homologue CopC from Chromobacterium violaceum, opportunistic but sometimes deadly pathogen. bears same ADP-riboxanase activity as OspC3, with different substrate specificity....
Pathogenic mycobacteria, such as Mycobacterium tuberculosis, bovis, and marinum, can trigger NLRP3 inflammasome activation leading to maturation secretion of interleukin 1β (IL-1β). However, the mycobacterial factors involved in are not fully understood. Here, we identified that PPE family protein PPE13 was responsible for induction IL-1β a inflammasome-dependent manner. We found recombinant smegmatis expressing activates inflammasome, thereby inducing caspase-1 cleavage J774A.1, BMDMs,...
Listeriolysin O (LLO) is a cholesterol-dependent cytolysin that mediates the escape of L. monocytogenes from phagosomes and enables bacteria to grow within host. LLO versatile tool allowing Listeria trigger several cellular responses. In this study, rapid phosphorylation ERK1/2 on Caco-2 cells caused by infection was demonstrated be highly dependent activity. The effect could strongly induced adding purified recombinant alone inhibited exogenous cholesterol. Lack PEST sequence, known tightly...
Microcin C7 (McC), widely distributed in enterobacteria, is a promising antibiotic against resistance [...]
Aminopeptidases that catalyze the removal of N-terminal residues from polypeptides or proteins are crucial for physiological processes. Here, we explore biological functions an M29 family aminopeptidase II Listeria monocytogenes (LmAmpII). We show LmAmpII contains a conserved catalytic motif (EEHYHD) is essential its enzymatic activity and has substrate preference arginine leucine. Studies on roles indicate required in vitro growth chemically defined medium optimal L. but not bacterial...
As a byproduct of mitochondrial respiration or metabolism, reactive oxygen species (ROS) can act as signaling molecule to activate NLR family pyrin domain containing 3 (NLRP3) inflammasome, thereby triggering immune response. NLRP3 inflammasome acts sensor various danger signals and is central the control pyroptosis occurrence. Macrophage closely related atherosclerosis, arthritis, pulmonary fibrosis other inflammatory diseases. Methylophiopogonanone A (MO-A) main homoisoflavonoid in Chinese...
Ketamine is commonly used for sedation, analgesia and anesthetics, many evidences have shown that it has an immune-regulatory effect. The cholinergic anti-inflammatory pathway mediated by α7nAChR a prominent target of therapy. But whether ketamine suppresses inflammatory levels in nerve cells activating remains unknown. Lipopolysaccharide (LPS) was to establish the neuroinflammation model PC12 vitro, siRNA transfected into 30 min before LPS inhibit gene expression α7nAChR. were stimulated...