A5037269840- Forensic and Genetic Research
- Psoriasis: Treatment and Pathogenesis
- Dermatology and Skin Diseases
- Molecular Biology Techniques and Applications
- Skin Protection and Aging
- Cutaneous lymphoproliferative disorders research
- Nail Diseases and Treatments
- Cutaneous Melanoma Detection and Management
- Nonmelanoma Skin Cancer Studies
- Autoimmune Bullous Skin Diseases
- melanin and skin pigmentation
- Forensic Anthropology and Bioarchaeology Studies
- Immunodeficiency and Autoimmune Disorders
- Acne and Rosacea Treatments and Effects
- Contact Dermatitis and Allergies
- Inflammasome and immune disorders
- Autopsy Techniques and Outcomes
- Genetic and rare skin diseases.
- Environmental DNA in Biodiversity Studies
- Autoimmune and Inflammatory Disorders
- Mast cells and histamine
- Bacterial Identification and Susceptibility Testing
- Urinary Tract Infections Management
- Drug-Induced Adverse Reactions
- Hedgehog Signaling Pathway Studies
Monash University
2025
National Skin Centre
2015-2024
Singapore General Hospital
2017
Guy's and St Thomas' NHS Foundation Trust
2015
St John's Hospital
2014
National Heart Centre Singapore
2012
University of Pennsylvania
2012
Nanyang Technological University
2011
ANDE (United States)
2009
Tan Tock Seng Hospital
2008
The term pustular psoriasis indicates a group of severe skin disorders characterized by eruptions neutrophil-filled pustules. disease, which often manifests with concurrent vulgaris, can have an acute systemic (generalized [GPP]) or chronic localized (palmoplantar pustulosis [PPP] and acrodermatitis continua Hallopeau [ACH]) presentation. Although mutations been uncovered in IL36RN AP1S3, the rarity disease has hindered study genotype-phenotype correlations.We sought to characterize clinical...
Prominent skin involvement is a defining characteristic of autoinflammatory disorders caused by abnormal IL-1 signaling. However, the pathways and cell types that drive cutaneous features remain poorly understood. We sought to address this issue investigating pathogenesis pustular psoriasis, model with predominant manifestations. specifically characterized impact mutations affecting AP1S3, disease gene previously identified our group validated here in newly ascertained patient resource....
Abstract In November 2018, Butte County, California, was decimated by the Camp Fire, deadliest wildfire in state history. Over 150,000 acres were destroyed, and at its peak, fire consumed eighty per minute. The speed intensity of oncoming flames killed scores people, weeks before contained, first responders began searching through rubble 18,804 residences commercial buildings. As with most mass disasters, conventional identification modalities (e.g., fingerprints, odontology, hardware)...
Abstract Background The generation of short tandem repeat profiles, also referred to as ‘DNA typing,’ is not currently performed outside the laboratory because process requires highly skilled technical operators and a controlled environment infrastructure with several specialized instruments. goal this work was develop fully integrated system for automated profiles from buccal swab samples, improve forensic flow well enable profile be in police stations field-forward military, intelligence,...
A developmental validation was performed to demonstrate reliability, reproducibility and robustness of the ANDE System with FlexPlex assay, including an integrated Expert System, across a number laboratories buccal sample variations. Previously, related DNAscan™/ANDE 4C Rapid DNA using PowerPlex®16 assay Software received NDIS approval in March 2016. The enhanced instrument, referred as 6C, accompanying 6-dye, 27-locus STR FlexPlex, have been developed be compatible all widely used global...
Abstract Background Crohn’s disease (CD) and ulcerative colitis (UC) have been associated with the major histocompatibility complex in genome wide association studies (1) subsequently this area is most widely studied genetic region. HLA-DRB1*01:03 has susceptibility to both CD UC (2), severe (3). However, frequency of allele known vary by ethnicity. We aimed determine HLA DRB1*01:03 a multi-ethnic Australian population its refractory disease. Methods At single centre, tertiary Inflammatory...
Short tandem repeat (STR) analysis of casework samples with low DNA content include those resulting from the transfer epithelial cells skin to an object (e.g., on a water bottle, or brim cap), blood spatter stains, and small bone tissue fragments. Low (LDC) are important in wide range settings, including disaster response teams assist victim identification family reunification, military operations identify friend foe, criminal forensics suspects exonerate innocent, medical examiner coroner...
Abstract A developmental validation was performed to demonstrate reliability, reproducibility, and robustness of the ANDE Rapid DNA Identification System for processing crime scene disaster victim identification (DVI) samples. total 1705 samples were evaluated, including blood, oral epithelial from drinking containers, on FTA untreated paper, semen, bone, soft tissues. This study conducted address FBI’s Quality Assurance Standards accumulate data a sufficient number unique donors sample...
Abstract: The time required for short tandem repeat (STR) amplification is determined by the temperature ramp rates of thermal cycler, components reaction mix, and properties vessel. Multiplex amplifications in microfluidic biochip‐based conventional tube‐based cyclers have been demonstrated 17.3 19 min, respectively. Optimized 28‐cycle protocols generated alleles with signal strengths above calling thresholds, heterozygous peak height ratios greater than 0.65, incomplete nontemplate...
Since the implementation of forensic DNA typing in labs more than 20 years ago, analysis procedures and data interpretation have always been conducted a laboratory by highly trained qualified scientific personnel. Rapid technology has potential to expand testing capabilities within laboratories allow STR be performed outside physical boundaries traditional laboratory. The developmental validation DNAscan/ANDE Analysis System was completed using BioChipSet™ Cassette consumable designed for...
SUMMARY A total of 131 new cases mycosis fungoides and Sézary syndrome were diagnosed clinically histopathologically at our centre over a 5‐year period. There 87 males 44 females with mean age 36.3 years (range 3–87 years) no racial predilection. Of the 62 patients (47.3%) classical fungoides, majority male (male : female = 4.2:1). was one patient syndrome. Patients aged older than 50 more likely to present longer duration symptoms advanced disease. In contrast 47 hypopigmented had early...
Abstract Phototherapy is a useful treatment modality for atopic dermatitis (AD). This prospective randomised double‐blind study comparing the clinical efficacy of combined ultraviolet‐A (UVA)/narrowband ultraviolet‐B (NBUVB) versus NBUVB phototherapy in chronic AD. Patients with moderate‐to‐severe AD were to receive either UVA/NBUVB or twice weekly over 12 weeks. At baseline, weeks 6 and 12, Eczema Area And Severity Index (EASI), itch score adverse effects assessed. baseline week...
Abstract Background/Objectives Susceptibility to and clinical presentation of basal cell carcinoma ( BCC ) differ in A sian C aucasian populations. This study aims evaluate the epidemiological clinicopathological characteristics a multiracial S ingaporean population, with secondary comparative analysis between hinese patients. Methods We prospectively studied patients newly diagnosed, histologically confirmed at N ational kin entre, ingapore from 2004 2008. Results In total, 274 260 were...
This work focuses on anthropomorphic exoskeletons for the human wrist. We consider a 2 dof model wrist with non intersecting joints and similar exoskeleton. assume viscoelastic attachment between hand handle of exoskeleton which one side allows different kinematics to follow and, other side, induces reaction forces at all joints, in particular causing discomfort. quantify discomfort as amount potential energy stored deformation attachment. For specific implementation, based kinematic...
Background The intentional release of Bacillus anthracis in the United States 2001 has heightened concern about use pathogenic microorganisms bioterrorism attacks. Many deadliest bacteria, including Class A Select Agents anthracis, Francisella tularensis, and Yersinia pestis, are highly infectious via pulmonary route when released aerosolized form. Hence, rapid, sensitive, reliable methods for detection these biothreats characterization their potential impact on exposed population critical...
Background Chlamydia trachomatis (Ct) is the most common cause of bacterial sexually transmitted diseases (STD) worldwide. While commercial nucleic acid amplification tests (NAAT) are available for Ct, none rapid or inexpensive enough to be used at point-of-care (POC). Towards first Ct POC NAAT, we developed a microfluidic assay that simultaneously interrogates nine loci in 20 minutes. Methodology and Principal Findings Endocervical samples were selected from 263 women high risk STDs (∼35%...
ABSTRACT Introduction: Nail psoriasis treatment is challenging due to difficult drug delivery and systemic therapy toxicities. Self-dissolvable microneedle patches embedded with corticosteroids offers a potentially rapid, minimally invasive platform good efficacy minimal adverse side effects. Methods: We conducted 4-month prospective randomised controlled trial. Subjects psoriatic nails were receive device delivered topical steroids on one hand control (topical Daivobet gel) the other. Two...
Photopatch testing represents the gold standard for diagnosis of photoallergic contact dermatitis (PACD). We aimed to identify common photoallergens in our tertiary dermatological referral centre from 2012 2021, compare this preceding period studied, and data other communities.We conducted a retrospective review all 90 patients who underwent photopatch at National Skin Centre, Singapore, between 2021.Of patients, 19 (21.1%) were male, mean age was 41.6 years. Eighty-four (93.3%) sunscreen...