A5026849953- Cerebral Venous Sinus Thrombosis
- Ophthalmology and Eye Disorders
- Intraocular Surgery and Lenses
- Retinal Diseases and Treatments
- Retinal and Optic Conditions
- Bacterial biofilms and quorum sensing
- Glaucoma and retinal disorders
- Nitric Oxide and Endothelin Effects
- Bacterial Genetics and Biotechnology
- Retinal Imaging and Analysis
- Attention Deficit Hyperactivity Disorder
- Endoplasmic Reticulum Stress and Disease
- Ocular Diseases and Behçet’s Syndrome
- Platelet Disorders and Treatments
- Health disparities and outcomes
- Neurological Complications and Syndromes
- Vibrio bacteria research studies
- Mobile Health and mHealth Applications
- Obesity, Physical Activity, Diet
- Protist diversity and phylogeny
- bioluminescence and chemiluminescence research
- Autophagy in Disease and Therapy
- Child Therapy and Development
- Connective tissue disorders research
- Attachment and Relationship Dynamics
Education University of Hong Kong
2023-2024
Chinese University of Hong Kong
2003-2023
Hong Kong Eye Hospital
2003-2023
National Institutes of Health
1993-2013
National Heart Lung and Blood Institute
2013
Women and Children’s Health Research Institute
2012
University of Alberta
2012
University of Basel
2004-2007
Institute of Physics
2005
The Netherlands Cancer Institute
2004
Pole development is coordinated with the Caulobacter crescentus cell cycle by two-component signaling proteins. We show that an unusual response regulator, PleD, required for polar differentiation and sequestered to pole only when it activated phosphorylation. Dynamic localization of PleD provides a mechanism temporally spatially control output during development. Targeting coupled activation C-terminal guanylate cyclase domain, which catalyzes synthesis cyclic di-guanosine monophosphate....
Recent discoveries suggest that a novel second messenger, bis-(3′→5′)-cyclic di-GMP (c-diGMP), is extensively used by bacteria to control multicellular behavior. Condensation of two GTP the dinucleotide catalyzed widely distributed diguanylate cyclase (DGC or GGDEF) domain occurs in various combinations with sensory and/or regulatory modules. The crystal structure unorthodox response regulator PleD from Caulobacter crescentus , which consists CheY-like receiver domains and DGC domain, has...
Initiation of protein folding by light can dramatically improve the time resolution kinetic studies. Here we present an example optically triggered reaction using nanosecond photodissociation heme-carbon monoxide complex reduced cytochrome c. The optical trigger is based on observation that under destabilizing conditions c be unfolded preferential binding carbon to covalently attached heme group in state. Photodissociation thus triggers reaction. We used time-resolved absorption spectroscopy...
Cellular mitochondrial content is governed by the competing processes of organelle biogenesis and degradation. It proposed that these programs are tightly regulated to ensure cell maintains sufficient organelles meet its biosynthetic, energetic, other homeostatic requirements. We recently reported GCN5L1, a putative nutrient-sensing regulator, controls removal autophagy. Here we show genetic deletion GCN5L1 has direct positive effect on expression activity Transcriptional Factor EB (TFEB),...
NO, produced by the endothelium, is a modulator of vascular inflammation. Traditionally, eNOS was believed to be primary source NO in endothelium. However, recent data suggest an important role for nNOS although little known about factors regulating this novel eNOS. We examined localization, regulation, and significance endothelial study. Primary HUVECs were used as model system. Inflammatory changes induced stimulation with TNF. report that unlike eNOS, predominantly localized nucleus...
<h3>Background/aims:</h3> To compare the optic disc parameters between patients with non-arteritic anterior ischaemic neuropathy (NAION) and normal controls, using optical coherence tomography (OCT) Heidelberg Retinal Tomograph III (HRT), to evaluate structure–function relationship in NAION eyes. <h3>Methods:</h3> Both eyes of 22 typical unilateral ⩾6 months' duration 52 from randomly selected subjects underwent Humphrey visual field (HVF) examination measurement retinal nerve fibre layer...
The quality of clinical trials is essential to advance treatment, inform regulatory decisions and meta-analysis. With the increased incidence idiopathic intracranial hypertension emergence for novel therapies in this condition, International Headache Society Guidelines Controlled Clinical Trials Idiopathic Intracranial Hypertension aims establish guidelines designing state-of-the-art controlled hypertension.
Purpose. Mutations in the NR2E3 and NRL genes have been implicated both autosomal dominant recessive retinitis pigmentosa (RP). In this study, mutation profiles of these two were investigated Chinese RP patients. Methods. 172 patients 360 normal control subjects (180 from Hong Kong 180 Beijing), coding exons exon-intron boundaries screened by direct DNA sequencing after PCR. Association analysis was performed for common single-nucleotide polymorphisms (SNPs), whereas silico programs used...
Recent studies suggest that a novel second messenger, cyclic d-GMP (c-diGMP), is extensively used by bacteria to control multicellular behaviour.This dinucleotide synthesised the diguanylate cyclase (DGC) domain in reaction converts two GTP into one c-diGMP and pyrophosphates.The DGC contains highly conserved GG(D/E)EF sequence motif, occurs various combinations with sensory / regulatory domains bacteria.We have identified response regulator, PleD, from Caulobacter crescentus as [1] solved...
Abstract Background and Aim Hepatitis B core‐related antigen (HBcrAg) has been shown to correlate with various viral markers in chronic hepatitis B, but its role defining natural history is not well studied. We aimed investigate the use of HBcrAg define different phases B. Methods Stored residual serum samples from longitudinal cohorts patients Hong Kong Japan were Viral measured three serial for each patient. Patients divided into six groups analysis: e (HBeAg)‐positive infection (EPI),...
In this retrospective case series, the authors described clinical features of 18 patients with parainfectious optic neuritis who presented to two neuro-ophthalmology practices in Israel between 2005 and 2012.In study, was defined as acute within 1 month after an infectious disease.Patients multiple sclerosis other causes neuropathy were excluded.Of patients, 10 children (mean age 12.7 years, 50% bilateral) 8 adults 31 38% bilateral).The male:female ratios both groups 1:1.All had disc...
Nicotinic acetylcholine receptors (nAChRs) mediate transmission of synaptic signals and contribute to learning memory capacities as well nicotine addiction in tobacco smokers.Signals are transmitted upon binding agonists the ligand domain opening transmembrane ion channel.nAChRs consist pentameric proteins site is located at interfaces between so-called principal complementary subunits.Acetylcholine (AChBPs) have been identified snails soluble homologs nAChRs.Recently, we solved crystal...
Acute retinal artery occlusion (RAO) is essentially a stroke in the eye; therefore, it shares same risk factors as acute cerebral infarctions.In fact, American Heart Association and Stroke recommend immediate brain imaging all patients with ischaemia.In this retrospective observational case series, authors reviewed results of diffusion-weighted (DW) magnetic resonance (MRI) brain, done within 7 days visual symptom onset, 33 consecutive RAO (18 central [CRAO], 15 branch [BRAO]).Acute...
Central visual acuity measurement is usually the primary test of vision, but when used alone, it may not correlate well with neurovisual dysfunction.Complete assessment function requires testing additional aspects, e.g., colour contrast sensitivity, and fields, which time-consuming.In this study, author proposed a new rapid screening for dysfunction, rarebit digit (RDT).Rarebits are receptive field-sized bright dots briefly presented on dark background (not to be confused dish melted cheese...
The optic nerve sheath diameter (ONSD) is generally increased in patients with intracranial pressure and decreased hypotension.Therefore, ONSD can be used to estimate noninvasively.ONSD measured by magnetic resonance imaging (MRI) or ultrasound.MRI provides high spatial resolution, but it costly may not readily available.On the other hand, orbital ultrasound performed equipment brought bedside.How reproducible are measurements?In this prospective observation study, right eyes of 15 healthy...