A5077945426- Autoimmune Neurological Disorders and Treatments
- Neuroscience and Neuropharmacology Research
- Genetics and Neurodevelopmental Disorders
- RNA regulation and disease
- Alzheimer's disease research and treatments
- RNA Research and Splicing
- Nuclear Structure and Function
- Cholinesterase and Neurodegenerative Diseases
- Plant Molecular Biology Research
- Endoplasmic Reticulum Stress and Disease
- Microtubule and mitosis dynamics
- Congenital Heart Disease Studies
- Developmental Biology and Gene Regulation
- Epilepsy research and treatment
- Cancer-related gene regulation
- Ultrasound in Clinical Applications
- 14-3-3 protein interactions
- Epigenetics and DNA Methylation
- Photosynthetic Processes and Mechanisms
- Genomics and Chromatin Dynamics
- Central Venous Catheters and Hemodialysis
- Advanced Biosensing Techniques and Applications
- Polyomavirus and related diseases
University of Pennsylvania
2008-2022
Children's Hospital of Philadelphia
2022
Brandeis University
2012-2016
Anti-N-methyl D-aspartate receptor (NMDAR) encephalitis is a severe neuropsychiatric disorder that associates with prominent memory and behavioural deficits. Patients' antibodies react the N-terminal domain of GluN1 (previously known as NR1) subunit NMDAR causing in cultured neurons selective reversible internalization cell-surface receptors. These effects frequent response to immunotherapy have suggested an antibody-mediated pathogenesis, but date there no animal model showing patients'...
Objective A severe but treatable form of immune‐mediated encephalitis is associated with antibodies in serum and cerebrospinal fluid (CSF) against the GluN1 subunit N‐methyl‐D‐aspartate receptor (NMDAR). Prolonged exposure hippocampal neurons to from patients anti‐NMDAR caused a reversible decrease synaptic localization function NMDARs. However, acute effects antibodies, fate internalized receptors, type affected, whether develop compensatory homeostatic mechanisms were unknown are focus...
Striated muscle fibers are characterized by their tightly organized cytoplasm. Here, we show that the Drosophila melanogaster KASH proteins Klarsicht (Klar) and MSP-300 cooperate in promoting even myonuclear spacing mediating a tight link between newly discovered nuclear ring polarized network of astral microtubules (aMTs). In either klar or msp-300ΔKASH, msp-300 double heterozygous mutants, aMTs retracted from envelope, abrogating this spacing. Anchoring myonuclei to core acto-myosin...
<h3>Objective</h3> To report 3 patients who developed anti–<i>N</i>-methyl-D-aspartate receptor encephalitis during pregnancy. <h3>Design</h3> Case reports. <h3>Setting</h3> University hospitals. <h3>Patients</h3> Three young women at 14, 8, and 17 weeks of gestation acute change behavior, prominent psychiatric symptoms, progressive decrease consciousness, seizures, dyskinesias, autonomic dysfunction. <h3>Main Outcome Measures</h3> Clinical, radiological, immunological findings....
The transcription factors of the Snail family are key regulators epithelial-mesenchymal transitions, cell morphogenesis, and tumor metastasis. Since its discovery in Drosophila ∼25 years ago, has been extensively studied for role as a transcriptional repressor. Here we demonstrate that can positively modulate activation. By combining information on vivo occupancy with expression profiling hand-selected, staged snail mutant embryos, identified 106 genes potentially directly regulated by...
OBJECTIVES: Umbilical venous cannulation is the favored approach to perinatal central access worldwide but has a failure rate of 25–50% and insertion technique not evolved in decades. Improving success this procedure would have broad implications, particularly where peripherally inserted catheters are easily obtained neonates with congenital heart disease, whom umbilical facilitates administration inotropes blood products while sparing vessels essential for later cardiac interventions. We...
ABSTRACT During bidirectional transport, individual cargoes move continuously back and forth along microtubule tracks, yet the cargo population overall displays directed net transport. How such transport is controlled temporally not well understood. We analyzed this issue for bidirectionally moving lipid droplets in Drosophila embryos, a system which direction developmentally controlled. By quantifying how droplet distribution changes as embryos develop, we characterize temporal transitions...
OBJECTIVE: To report an animal model of memory and behavioral deficits caused by antibodies from patients with anti-NMDAR encephalitis. BACKGROUND: Patients encephalitis develop prominent deficits. We previously showed that patient's cause a specific decrease the density, synaptic localization, function NMDARs in cultures neurons rat brain. However, confirmation antibody-mediated pathogenesis depends on modeling symptoms animals. METHODS: C57/BL6 mice underwent infusion patients' or control...
We recently identified CALHM1, a potential susceptibility gene for Alzheimer's disease (AD) located on chromosome 10, which encodes novel protein involved in Ca2+ homeostasis regulation. CALHM1 was using expressed sequence tag database (dbEST) mining identifying uncharacterized genes preferentially the hippocampus and linkage regions late-onset AD (LOAD). A common polymorphism found to cause profound impairments APP processing, significantly over-represented LOAD subjects large case-control...