A5078181157- Multiple Myeloma Research and Treatments
- Digital Imaging for Blood Diseases
- RNA Research and Splicing
- 3D Printing in Biomedical Research
- RNA modifications and cancer
- Garlic and Onion Studies
- Single-cell and spatial transcriptomics
- CAR-T cell therapy research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Immune Cell Function and Interaction
- Pomegranate: compositions and health benefits
- Protein Degradation and Inhibitors
- Acute Myeloid Leukemia Research
- Pineapple and bromelain studies
- AI in cancer detection
- Down syndrome and intellectual disability research
- Hematopoietic Stem Cell Transplantation
- Micro and Nano Robotics
- Cancer Mechanisms and Therapy
- Cell Image Analysis Techniques
- Retinoids in leukemia and cellular processes
- Histone Deacetylase Inhibitors Research
- Antioxidant Activity and Oxidative Stress
- Microfluidic and Capillary Electrophoresis Applications
- vaccines and immunoinformatics approaches
Hospital Universitario 12 De Octubre
2017-2025
Universidad Complutense de Madrid
2018-2025
Centro de Investigación Biomédica en Red de Cáncer
2020-2024
Research Institute Hospital 12 de Octubre
2018-2024
Spanish National Cancer Research Centre
2019-2023
Universidad del Valle
2018
Abstract CAR-T-cell therapy against MM currently shows promising results, but usually with serious toxicities. CAR-NK cells may exert less toxicity when redirected resistant myeloma cells. CARs can be designed through the use of receptors, such as NKG2D, which recognizes a wide range ligands to provide broad target specificity. Here, we test this approach by analyzing antitumor activity activated and expanded NK (NKAE) CD45RA − T from patients that were engineered express an NKG2D-based CAR....
Refractory/relapsed pediatric acute leukemia are still clinically challenging and new therapeutic strategies needed. Interactions between Natural Killer Group 2D (NKG2D) receptor, expressed in cytotoxic immune cells, its ligands (NKG2DL), which upregulated leukemic blasts, important for anti-leukemia immunosurveillance. Nevertheless, cells may develop immunoescape as NKG2DL shedding and/or downregulation.In this report, we analyzed the activity of NKG2D chimeric antigen receptor (CAR)...
Gut microbiota-derived urolithins may influence multiple myeloma (MM) disease progression and treatment. We analyzed their associated microbiota in a retrospective cohort of 45 patients with active MM or premalignant using mass spectrometry 16S rRNA gene sequencing. Patients detectable levels urolithin serum stool higher abundance urolithin-related had better outcome. Analysis the effects A (UroA) treatment ex vivo, vitro, vivo revealed that UroA is cytotoxic against cell lines modulates...
Abstract Analysis of bone marrow aspirates (BMAs) is an essential step in the diagnosis hematological disorders. This analysis usually performed based on a visual examination samples under conventional optical microscope, which involves labor-intensive process, limited by clinical experience and subject to high observer variability. In this work, we present comprehensive digital microscopy system that enables BMA for cell type counting differentiation efficient objective manner. not only...
FMS-like tyrosine kinase 3 (FLT3) is a key driver of acute myeloid leukemia (AML). Several inhibitors (TKIs) targeting FLT3 have been evaluated clinically, but their effects are limited when used in monotherapy due to the emergence drug-resistance. Thus, better understanding drug-resistance pathways could be good strategy explore and evaluate new combinational therapies for AML. Here, we phosphoproteomics identify differentially-phosphorylated proteins patients with AML TKI resistance. We...
Routine field diagnosis of malaria is a considerable challenge in rural and low resources endemic areas mainly due to lack personnel, training sample processing capacity. In addition, differential Plasmodium species has high level misdiagnosis. Real time remote microscopical through on-line crowdsourcing platforms could be converted into an agile network support diagnosis-based treatment control areas. This study explores whether accurate identification-a critical step during the protocol...
Mitochondria are involved in the development and acquisition of a malignant phenotype hematological cancers. Recently, their role pathogenesis multiple myeloma (MM) has been suggested to be therapeutically explored. MYC is master regulator b-cell malignancies such as myeloma, its activation known deregulate mitochondrial function. We investigated impact activity on distinct entities disease tested efficacy inhibitor, tigecycline, overcome MM proliferation. COXII expression, COX activity,...
Abstract Despite the approval of several drugs for AML, cytarabine is still widely used as a therapeutic approach. However, 85% patients show resistance and only 10% overcome disease. Using RNA-seq phosphoproteomics, we that RNA splicing serine-arginine-rich (SR) proteins phosphorylation were altered during resistance. Moreover, SR at diagnosis significantly lower in responder than non-responder patients, pointing to their utility predict response. These changes correlated with...
Current World Health Organization recommendations for the management of malaria include need a parasitological confirmation prior to triggering appropriate treatment. The use rapid diagnostic tests (RDTs) has contributed better infection recognition and more targeted Nevertheless, low-density infections parasites that fail produce HRP2 can cause false-negative RDT results. Microscopy traditionally been methodology most commonly used quantify characterize infecting species, but wider this...
We previously reported a new approach for micromanipulation and encapsulation of human stem cells using droplet-based microfluidic device demonstrated the possibility encapsulating culturing difficult-to-preserve primary hematopoietic an engineered double layered bead composed by inner layer alginate outer puramatrix constructed soft technology without use any external force. In this work, we micro manipulation technique to build 3D scaffold as biomimetic model recapitulate niche...
We previously reported a new approach for micromanipulation and encapsulation of human stem cells using droplet-based microfluidic device. This demonstrated the possibility encapsulating culturing difficult-to-preserve primary hematopoietic an engineered double-layered bead composed by inner layer alginate outer Puramatrix. also maintenance expansion Multiple Myeloma in this construction. Here, presented technique is applied to construct 3D biomimetic model recapitulate cell niche hydrogel...
Many studies over the last 20 years have investigated role of mitochondrial DNA (mtDNA) alterations in carcinogenesis. However, status mtDNACN MM and its implication pathogenesis disease remains unclear. We examined changes plasma cell across different stages by applying RT-PCR high-throughput sequencing analysis. observed a significant increase average myeloma cells compared with healthy (157 vs. 40 copies; p = 0.02). also found an SMM newly diagnosed (NDMM) paired samples consecutive...
Regenerative medicine has increasingly made use of adult stem cells in the last years (1, 2). Micro-engineering a biomimetic three dimensional structure provides realistic approach for cell niche studies, and further translational applications. A promising engineering artificial niches is provided by high-throughput microfluidic technologies. In this work, droplet-based microfluidic-assisted encapsulation device generation multi-layered cellular structures on demand using alginate Puramatrix...
Abstract Analysis of bone marrow aspirates (BMA) is an essential step in the diagnosis hematological disorders. This analysis usually performed based on visual examination samples under a conventional optical microscope, which involves labor-intensive process, limited by clinical experience and subject to high observer variability. In this work, we present comprehensive digital system that enables BMA for cell type counting differentiation efficient objective manner. not only provides...
Abstract The nucleolus is a dynamic structure where ribosome subunits are produced. Indeed, nucleoli respond to any change in cellular homeostasis by altering the rate of biogenesis, thus working as stress sensor. Therefore, an imbalance biogenesis promotes changes morphology and function can evoke nucleolar response. Changes composition impair have been described stress, mechanism related aging cancer. Here, we show role RNA binding protein Hnrnpk dynamics function. ribonucleoprotein charge...
Background: Protein biogenesis is a complex multistep process involving nucleoli and ribosomes. Aberrations in any step could lead to alterations protein output. hnRNP K an RNA binding (RBP) that nascent RNAs into mature mRNAs we found its overexpression results nuclear nucleolar abnormalities, decrease transcription potentially promotes imbalance ribosome biogenesis, leading translation. Aims: We aim elucidate how dysregulation impact on HSC biology. Methods: To study the of vivo, developed...