A5001404888- Gut microbiota and health
- Antiplatelet Therapy and Cardiovascular Diseases
- Diet and metabolism studies
- Atrial Fibrillation Management and Outcomes
- Clostridium difficile and Clostridium perfringens research
- Platelet Disorders and Treatments
- Aortic Disease and Treatment Approaches
- Adipose Tissue and Metabolism
- Aortic aneurysm repair treatments
- Hormonal and reproductive studies
- Cardiovascular Function and Risk Factors
- Blood properties and coagulation
- Nitric Oxide and Endothelin Effects
- Sexual function and dysfunction studies
- Cardiovascular Health and Disease Prevention
- Lipoproteins and Cardiovascular Health
- Renal Transplantation Outcomes and Treatments
- Venous Thromboembolism Diagnosis and Management
- Muscle metabolism and nutrition
- Nutritional Studies and Diet
- Ovarian cancer diagnosis and treatment
- BRCA gene mutations in cancer
- Acute Myocardial Infarction Research
- Tryptophan and brain disorders
- Biosimilars and Bioanalytical Methods
Hospital Clínico San Carlos
2010-2024
Centro de Investigación en Red en Enfermedades Cardiovasculares
2020-2024
Instituto de Investigación Sanitaria del Hospital Clínico San Carlos
2012-2024
Centro de Investigación Biomédica en Red
2022-2024
Instituto de Salud Carlos III
2022-2024
Spanish National Centre for Cardiovascular Research
2013-2023
Universidad Complutense de Madrid
2013-2022
Centre for Biomedical Network Research on Rare Diseases
2020
Universidad de Alcalá
2019
Czech Academy of Sciences, Institute of Physiology
2015
Microcirculatory alterations displayed by patients with heart failure (HF) induce structural and functional intestinal changes that may affect normal gut microbial community. At the same time, microbiota can influence pathological mechanisms implicated in HF progression. However, it is unknown whether dysbiosis precede development of cardiac or only a mere consequence. Our aim was to investigate potential relationship between composition comparing spontaneously hypertensive rat models. Gut...
It remains unknown whether the presence of coronary microcirculatory dysfunction (CMD) correlates with its equivalent condition in brain, cerebral small vessel disease (CSVD). The cerebral-coronary connection (C3), a prospective blinded study, investigated prevalence CMD patients artery (CAD) and association CSVD cognitive function.Patients documented CAD fulfilling inclusion criteria underwent physiological assessment epicardial vessels microcirculation using intracoronary pressure Doppler....
There is a lack of direct evidence regarding gut microbiota dysbiosis and changes in short-chain fatty acids (SCFAs) heart failure (HF) patients. We sought to assess any association between composition, SCFA production, clinical parameters, the inflammatory profile cohort newly diagnosed HF In this longitudinal prospective study, we enrolled eighteen At admission after 12 months, blood samples were collected for assessment proinflammatory cytokines, monocyte populations, endothelial...
The aim of the present study was to analyse differences in protein expression profile between platelets from aspirin (ASA)-resistant patients and ASA-sensitive patients. We analysed 51 clinically stable coronary ischaemic taking ASA (100 mg/day) divided into ASA-resistant (n=25) (n=26) based on a platelet functionality test (PFA-100). Proteins associated with cytoskeleton, energetic metabolism, oxidative stress, inflammation cell survival were by two-dimensional electrophoresis mass...
Acute coronary syndromes (ACS) are associated with platelet activation. The aim of the present study was to protein expression level glycolysis, oxidative stress, cytoskeleton and cell survival in platelets obtained during an ACS. Platelets from 42 ischemic patients, divided into patients admitted within 24 h after onset chest pain (ACS group; n=16) stable disease (CAD, n=26), were analyzed using proteomics. levels proteins involved cellular (F-actin capping, β-tubulin, α-tubulin isotypes 1...
Abstract Electromechanical characterization during atrial fibrillation (AF) remains a significant gap in the understanding of AF-related myopathy. This study reports mechanistic insights into electromechanical remodeling process associated with AF progression and further demonstrates its prognostic value clinic. In pigs, sequential assessment shows progressive decrease mechanical activity early dissociation from electrical counterpart. Atrial tissue samples animals reveal an abnormal...
Proteomics and Atrial Appendages. Introduction: The objective was to compare by proteomics the expression of proteins associated with cytoskeleton, energetic metabolism, cardiac cytoprotection between left atrial appendages (LAA) right (RAA) obtained from patients mitral valve disease both in sinus rhythm (SR, n = 6) permanent fibrillation (AF, 11). Methods Results: Samples RAA LAA were same patient. Proteins separated 2‐dimensional electrophoresis identified mass spectrometry. SR...
Background: Different works have suggested that in the hypertrophied heart energy metabolic pathway shifts to glycolysis. Our aim was evaluate using proteomics expression of proteins associated with different energetic pathways left ventricles spontaneously hypertensive rats (SHR). Methods: 24-weeks-old SHR stable hypertension and established ventricle hypertrophy were used. Normotensive Wistar Kyoto used as control. Proteins from analyzed by 2-dimensional electrophoresis identified...
Patients with subaneurysmal aortic dilation (SAD; 25–29 mm diameter) are likely to progress true abdominal aneurysm (AAA). Despite these patients having a higher risk of all-cause mortality than subjects size <24 mm, early diagnostic biomarkers lacking. MicroRNAs (miRs) well-recognized potential due their differential expression in different tissues and stability blood. We have investigated whether plasma miRs profile could identify the presence SAD high cardiovascular patients. Using...
There is interest to analyse newer biomarkers identify healthy individuals at risk develop cardiovascular disease (CVD) incidents and death. To determine in new circulating protein biomarkers, whose systemic levels may be associated with the of future development CVD The study was performed 82 from Malmö Diet Cancer cohort, free whom 41 developed did not. Plasma proteins related inflammation thrombo-coagulating processes were analysed. α1-antitrypsin isotype 3 plasma significantly higher...
Stroke patients have a high risk of vascular recurrence. Biomarkers related to recurrence, however, remain be identified. The aim the study was identify, through proteomic analysis, plasma biomarkers associated with recurrence within one year after first ischemic stroke. This is substudy (n = 134) large prospective multicenter post-stroke an Plasma samples were obtained at inclusion. Among identified proteins, only levels desmoplakin I protection against new event (Odds ratio: 0.64; 95% CI:...
Objective To analyse if platelet responsiveness to aspirin (ASA) may be associated with a different ability of platelets generate nitric oxide (NO). Patients/Methods Platelets were obtained from 50 patients stable coronary ischemia and divided into ASA-sensitive (n = 26) ASA-resistant 24) using functionality test (PFA-100). Results tended release more NO (determined as nitrite + nitrate) than but it did not reach statistical significance. Protein expression synthase 3 (NOS3) was higher in...
Several mechanisms have been proposed to explain why some platelets a reduced response aspirin (ASA). Among them, it was reported an increased circulating level of vitamin-D-binding protein (DBP). In addition, nitric oxide (NO) released from mononuclear cells involved in the antiplatelet effects ASA. The aim analyse relationship between platelet ASA and both NO generation content cells.Mononuclear were obtained patients with stable coronary artery disease that divided by functionality test...
Thromboembolic events are the second cause of death in cancer patients, although mechanisms underlying this increased thromboembolic risk remain unclear. The aims study were to examine whether BRCA2 gene mutations may modify circulating levels thrombocoagulation biomarkers and breast development influence changes such biomarkers. was performed 25 women with (n=12 cancer, n=13 cancer-free) 13 non-mutant controls. Results revealed that plasma fibrinogen gamma chain isotypes 2 3, haptoglobin 4...
It has been suggested that activated brown adipose tissue (BAT) shows increased glucose metabolic activity. However, less is known about activity of BAT under conditions fasting and normal temperature. The aim this study was to compare the possible differences in energetic metabolism between white (WAT) obtained from rabbits physiological temperature 24 h after conditions. carried out on New Zealand ( n =10) maintained for a period 8 weeks at 23±2 °C. Food removed before WAT were obtained....
Further to its pivotal role in haemostasis, factor Xa (FXa) promotes effects on the vascular wall. The purpose of study was evaluate if FXa modifies expression level energy metabolism and oxidative stress-related proteins femoral arteries obtained from type 2 diabetic patients with end-stage vasculopathy.Femoral were 12 who underwent leg amputation. Segments incubated vitro alone presence 25 nmol l(-1) + 50 rivaroxaban.In arteries, increased triosephosphate isomerase...
Background Most hypertensive patients, despite a proper control of their cardiovascular risk factors, have complications, evidencing the importance controlling and/or reversing target-organ damage. In this sense, endothelial dysfunction has been associated with presence factors and related outcomes. Since hypertension often clusters other such as dyslipemia, diabetes obesity, in study we investigated effect intensive multifactorial treatment on circulating vascular progenitor cell levels...