Remote-controlled manipulation of gene editing was achieved in vivo.

Near-infrared (NIR)-light-triggered photothermal therapy (PTT) is usually associated with undesirable damage to healthy organs nearby due the high temperatures (>50 °C) available for tumor ablation. Low-temperature PTT would therefore have tremendous value clinical application. Here, we construct a hypoxia-responsive gold nanorods (AuNRs)-based nanocomposite of CRISPR-Cas9 mild-photothermal via tumor-targeted gene editing. AuNRs are modified azobenzene-4,4'-dicarboxylic acid (p-AZO) achieve...

Cuproptosis has drawn enormous attention in antitumor material fields; however, the responsive activation of cuproptosis against tumors using nanomaterials with high atom utilization is still challenging. Herein, a copper-based nanoplatform consisting acid-degradable copper hydride (CuH) nanoparticles was developed via microfluidic synthesis. After coating tumor-targeting hyaluronic acid (HA), denoted as HA-CuH-PVP (HCP) shows conspicuous damage toward tumor cells by generating Cu+ and...

CRISPR/Cas12a has shown great potential in molecular diagnostics, but its application sensing of microRNAs (miRNAs) was limited by sensitivity and complexity. Here, we have sensitively conveniently detected reasonably integrating metal–organic frameworks (MOFs) based biobarcodes with assay (designated as MBCA). In this work, DNA-functionalized Zr-MOFs were designed the converter to convert amplify each miRNA target into activators that can initiate trans-cleavage activity further signal....

Tumor whole cell, carrying a complete set of tumor-associated antigens and tumor-specific antigens, has shown great potential in the construction tumor vaccines but is hindered by complex engineering means limited efficacy to cause immunity. Herein, we provided strategy for self-mineralization autologous cells with palladium ions microfluidic droplets, which endowed engineered both immune catalytic functions, establish bioorthogonally whole-cell vaccine. This vaccine showed strong inhibition...

Type 2 diabetes (T2D) results from the cells' insulin resistance, and to date, therapy medications targeting glycemic management have failed reverse increase in T2D prevalence. Restoring liver functions improve hepatic resistance by reducing oxidative stress is a potential strategy for treatment. Herein, liver-targeted biodegradable silica nanoshells embedded with platinum nanoparticles (Pt-SiO2) are designed as reactive oxygen species (ROS) nanoscavengers functional hollow nanocarriers....

Targeted construction of therapeutic nanoplatforms in tumor cells with specific activation remains appealing but challenging. Here, we design a cancer-motivated upconversion nanomachine (UCNM) based on porous nanoparticles (p-UCNPs) for precise phototherapy. The nanosystem is equipped telomerase substrate (TS) primer and simultaneously encapsulates 5-aminolevulinic acid (5-ALA) d-arginine (d-Arg). After coating hyaluronic (HA), it can readily get into cells, where 5-ALA induces efficient...

Abstract Inflammatory bowel disease (IBD) is a chronic relapsing autoimmune that characterized by segmental intestinal inflammation. There an urgent need for more efficient inflammation‐targeting strategies to improve therapeutic effect and reduce systemic drug exposure. Herein, oxidation‐responsive metal–organic framework material (Ce‐MOF@PSS) reported preferentially adheres inflamed intestine via enema. The overproduced reactive oxygen species (ROS) at inflammatory sites induces...

Abstract As a promising therapeutic modality targeting cancer, gas therapy still faces critical challenges, especially in enhancing efficacy and avoiding poisoning risks. Here, pH/glutathione (GSH) dual stimuli‐responsive CRISPR/Cas9 gene‐editing nanoplatform combined with calcium‐enhanced CO for precise anticancer therapy, is established. In the tumor microenvironment (TME), fast biodegradation of CaCO 3 layer via pH‐induced hydrolyzation allows glucose oxidase (GO x ) to catalyze H 2 O...

Cancer has become a leading cause of morbidity and mortality, there is an increasing need for versatile tools to enable sensitive, simple early cancer monitoring. Here, we report platinum supernanoparticles as exogenous nanosensor which can dissociate into ultrasmall nanoclusters (PtNCs) under tumor-specific hypoxia conditions. The resulting PtNCs be filtered through the kidney urinary reporters quantified by companion volumetric bar-chart chip (V-Chip) point-of-care analysis. V-Chip signals...

Early detection and precise tumor localization are critical for improving treatment outcomes enabling more targeted minimally invasive therapies as biotechnology evolves. However, endogenous biomarkers from early lesions face significant challenges, such short circulation times blood dilution, which hinder diagnostic efforts. In this study, we present a multimodal nanosensor specifically engineered to target cancer by responding CD44 tumor-associated enzymes within the microenvironment....

Abstract Polyamines have tantalized cancer researchers as a potential means to rein in the rampant growth of cells. However, clinical trials recent decades disappointed delivering notable progress. Herein, microfluidic‐assisted synthetic hydrogen‐bond organic framework (HOF) polyamine‐depleting nanoplatforms designed unleash vigor both dendritic cells (DCs) and T for precision immunotherapy is reported. Upon internalization by tumor cells, loaded plasma amine oxidase (PAO) HOF efficiently...

Abstract Renal clearance is critical for nanodrug to avoid the long‐term body retention‐related side effects, while it difficult achieve efficient tumor accumulation and retention. The over‐developed lysosomes in cancer cells have become an emerging target more precise effective therapy. Herein, a pH‐responsive reversible self‐assembled Cu 2‐ x Se‐BSA, which can be renal cleared under neutral or basic conditions, developed selectively targeted aggregation with enhanced Moreover, of Se‐BSA...

CRISPR/Cas12a shows excellent potential in disease diagnostics. However, insensitive signal conversion strategies hindered its application detecting protein biomarkers. Here, we report a metal–organic framework (MOF)-based DNA bio-barcode integrated with the system for ultrasensitive detection of In this work, zirconium-based MOF nanoparticles were comodified antibodies and phosphorylated as an efficient converter, which not only recognized biomarker to form sandwich complex but also...

Near-infrared (NIR)-light-triggered nanomedicine, including photodynamic therapy (PDT) and photothermal (PTT), is growing an attractive approach for cancer due to its high spatiotemporal controllability minimal invasion, but the tumor eradication limited by intrinsic anti-stress response of cells. Herein, we fabricate a tumor-microenvironment responsive CRISPR nanoplatform based on oxygen-deficient titania (TiO

Abstract Psoriasis is a chronic skin inflammation characterized by dysregulated crosstalk between immune cells and keratinocytes. Here we show that the cyclic GMP‐AMP synthase (cGAS)‐stimulator of interferon genes (STING) pathway key regulator psoriatic in mouse model. Platinum‐doped positively charged carbon dots (Pt‐CDs) were designed to inhibit cGAS‐STING pathway. By inhibiting with Pt‐CDs, secretion proinflammatory cytokines macrophages was reduced, cytokines‐induced breakdown...

Recent innovations in nanomaterials inspire abundant novel tumor-targeting CRISPR-based gene therapies. However, the therapeutic efficiency of traditional targeted nanotherapeutic strategies is limited by that biomarkers vary a spatiotemporal-dependent manner with tumor progression. Here, we propose self-amplifying logic-gated editing strategy for gene/H

Abstract Near‐infrared (NIR)‐light‐triggered photothermal therapy (PTT) is usually associated with undesirable damage to healthy organs nearby due the high temperatures (>50 °C) available for tumor ablation. Low‐temperature PTT would therefore have tremendous value clinical application. Here, we construct a hypoxia‐responsive gold nanorods (AuNRs)‐based nanocomposite of CRISPR‐Cas9 mild‐photothermal via tumor‐targeted gene editing. AuNRs are modified azobenzene‐4,4′‐dicarboxylic acid ( p...