A5091891066- Extracellular vesicles in disease
- Chronic Kidney Disease and Diabetes
- Renal Diseases and Glomerulopathies
- Acute Kidney Injury Research
- Inflammasome and immune disorders
- Renal and related cancers
- MicroRNA in disease regulation
- Parathyroid Disorders and Treatments
- Dialysis and Renal Disease Management
- Liver Disease Diagnosis and Treatment
- Connective Tissue Growth Factor Research
- Renal and Vascular Pathologies
- Renin-Angiotensin System Studies
- Advanced Biosensing Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Phagocytosis and Immune Regulation
- Heme Oxygenase-1 and Carbon Monoxide
- Pregnancy and preeclampsia studies
- Viral Infections and Vectors
- Muscle and Compartmental Disorders
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Kruppel-like factors research
- Monoclonal and Polyclonal Antibodies Research
- Genetic Syndromes and Imprinting
Zhongda Hospital Southeast University
2016-2025
Southeast University
2011-2024
Zhongshan Hospital
2024
Chinese University of Hong Kong
2015-2017
Chinese University of Hong Kong, Shenzhen
2015-2017
St. Paul's Hospital
2012
Micro (mi)RNAs are frequently dysregulated in the development of renal fibrosis. Exosomes small membrane vesicles that could be isolated from urine secreted all nephron segments. Here we sought to observe for first time whether miRNA exosome serve as a potential biomarker Urine samples were collected 32 chronic kidney disease (CKD) patients who underwent biopsy and 7 controls. Exosome was confirmed by immunogold staining marker. Members miR-29, miR-200, RNU6B endogenous control detected RT...
Recently, extracellular vesicles (EVs) have been attracting strong research interest for use as natural drug delivery systems. We report an approach to manufacturing interleukin-10 (IL-10)-loaded EVs (IL-10+ EVs) by engineering macrophages treating ischemic acute kidney injury (AKI). Delivery of IL-10 via enhanced not only the stability IL-10, but also its targeting due adhesive components on EV surface. Treatment with IL-10+ significantly ameliorated renal tubular and inflammation caused...
Mesenchymal stem cells-derived exosomes (MSC-exos) have attracted great interest as a cell-free therapy for acute kidney injury (AKI). However, the
Abstract Inflammation is a major contributor to the pathogenesis of ischemic acute kidney injury (AKI), which complicates post-operative outcomes large numbers hospitalized surgical patients. Hydroxychloroquine (HCQ), well-known anti-malarial drug, commonly used in clinical practice for its anti-inflammatory actions. However, little known about role renal ischemia/reperfusion (I/R) injury. In current study, mice were subjected I/R and HCQ was administered seven days by gavage prior surgery....
Exosomes derived from mesenchymal stem cells (MSC-exos) have been demonstrated with great potential in the treatment of multiple human diseases including acute kidney injury (AKI) by virtue their intrinsic cargoes. However, there are major challenges low yield and lack an established biomanufacturing platform to efficiently produce MSC-exos, thereby limiting therapeutic application. Here, we aimed establish a novel strategy MSC-exos hollow fiber bioreactor-based three-dimensional (3D)...
Recent studies indicate that microRNA (miRNA) is contained within exosome.Here we sought to optimize the methodologies for isolation and quantification of urinary exosomal as a prelude biomarker discovery studies.Exosomes were isolated through ultracentrifugation characterized by immunoelectron microscopy.To determine RNA was confined inside exosomes, pellet treated with RNase before isolation.The minimum urine volume, storage conditions exosomes miRNA evaluated.The presence miRNAs in...
Although glucocorticoids are the mainstays in treatment of renal diseases for decades, dose dependent side effects have largely restricted their clinical use. Microvesicles (MVs) small lipid-based membrane-bound particles generated by virtually all cells. Here we show that RAW 264.7 macrophage cell-derived MVs can be used as vectors to deliver dexamethasone (named MV-DEX) targeting inflamed kidney efficiently. Methods: macrophages were incubated with and then MV-DEX was isolated from...
Transforming growth factor β (TGF-β)/Smad3 signaling plays a role in tissue fibrosis. We report here that Erbb4-IR is novel long non-coding RNA (lncRNA) responsible for TGF-β/Smad3-mediated renal fibrosis and specific therapeutic target chronic kidney disease. was induced by TGF-β1 via Smad3-dependent mechanism highly upregulated the fibrotic of mouse unilateral ureteral obstructive nephropathy (UUO). Silencing blocked TGF-β1-induced collagen I alpha-smooth muscle actin (α-SMA) expressions...
IgA nephropathy (IgAN) features variable renal pathology and a heterogeneous clinical course. Our aim was to search noninvasive biomarkers from urinary exosomes for IgAN patients; membrane minimal change disease were included as other glomerulopathy controls. Transmission electron microscopy nanoparticle tracking analysis confirmed the size morphology characteristic of exosomes. Exosome markers (Alix CD63) well cell [aquaporin 2 (AQP2) nephrin] detected, which indicate origin excretion...
miR-155 was synthesized and loaded into exosomes in increased infiltration of macrophages a uremic heart. The released exosomal fusion with the plasma membrane leads to release cytosol translational repression forkhead transcription factors O class (FoxO3a) cardiomyocytes. Finally, macrophage-derived miR-155-containing promoted cardiomyocyte pyroptosis cardiomyopathy changes (cardiac hypertrophy fibrosis) by directly targeting FoxO3a mice.
Significance Statement AKI is a frequent clinical problem without definitive therapies. We developed an efficient RNAi therapy against by engineering red blood cell-derived extracellular vesicles (REVs) with targeting peptides and therapeutic siRNAs. REVs targeted Kim-1–binding peptide LTH efficiently delivered P65 Snai1 siRNAs to the injured tubules, leading reduced expression of P-p65 Snai1. Dual suppression inhibited renal inflammation fibrosis in mice subjected ischemia/reperfusion...
Background Podocyte injury and subsequent excretion in urine play a crucial role the pathogenesis progression of diabetic nephropathy (DN). Quantification messenger RNA (mRNA) expression urinary sediment by real-time PCR is emerging as noninvasive method screening DN-associated biomarkers. We hypothesized that mRNA profile podocyte-associated molecules may provide important clinical insight into different stages nephropathy. Methods DN patients (N = 51) healthy controls 13) were enrolled...
Dysfunctional mitochondria participate in the progression of chronic kidney disease (CKD). Pirfenidone is a newly identified anti-fibrotic drug. However, its mechanism remains unclear. Mitochondrial dysfunction an early event that occurs prior to onset renal fibrosis. In this context, we investigated protective effect pirfenidone on and relevance apoptosis oxidative stress proximal tubular cells. A remnant rat model was established. Human epithelial cells (HK2) using rotenone, mitochondrial...