A5101761402- Extracellular vesicles in disease
- Immune cells in cancer
- Acute Kidney Injury Research
- Phagocytosis and Immune Regulation
- Chronic Kidney Disease and Diabetes
- Macrophage Migration Inhibitory Factor
- Renal Diseases and Glomerulopathies
- Renal and related cancers
- Renal and Vascular Pathologies
- Cancer, Hypoxia, and Metabolism
- Heme Oxygenase-1 and Carbon Monoxide
- Dialysis and Renal Disease Management
- Chemotherapy-induced organ toxicity mitigation
- Renal cell carcinoma treatment
- Genetic Syndromes and Imprinting
- Advanced Electron Microscopy Techniques and Applications
- Ferroptosis and cancer prognosis
- MicroRNA in disease regulation
- Advanced Fluorescence Microscopy Techniques
- Epigenetics and DNA Methylation
- Muscle and Compartmental Disorders
- Arsenic contamination and mitigation
- Abdominal vascular conditions and treatments
- Erythropoietin and Anemia Treatment
- Galectins and Cancer Biology
Taizhou People's Hospital
2020-2024
Nantong University
2020-2024
Nanjing Medical University
2022-2024
Zhongda Hospital Southeast University
2018-2023
State Key Laboratory of Digital Medical Engineering
2023
Southeast University
2019-2023
Northern Jiangsu People's Hospital
2022
Affiliated Hospital of Nantong University
2021
Binzhou University
2020
Binzhou Medical University
2010-2020
Recently, extracellular vesicles (EVs) have been attracting strong research interest for use as natural drug delivery systems. We report an approach to manufacturing interleukin-10 (IL-10)-loaded EVs (IL-10+ EVs) by engineering macrophages treating ischemic acute kidney injury (AKI). Delivery of IL-10 via enhanced not only the stability IL-10, but also its targeting due adhesive components on EV surface. Treatment with IL-10+ significantly ameliorated renal tubular and inflammation caused...
Mesenchymal stem cells-derived exosomes (MSC-exos) have attracted great interest as a cell-free therapy for acute kidney injury (AKI). However, the
Exosomes derived from mesenchymal stem cells (MSC-exos) have been demonstrated with great potential in the treatment of multiple human diseases including acute kidney injury (AKI) by virtue their intrinsic cargoes. However, there are major challenges low yield and lack an established biomanufacturing platform to efficiently produce MSC-exos, thereby limiting therapeutic application. Here, we aimed establish a novel strategy MSC-exos hollow fiber bioreactor-based three-dimensional (3D)...
Although glucocorticoids are the mainstays in treatment of renal diseases for decades, dose dependent side effects have largely restricted their clinical use. Microvesicles (MVs) small lipid-based membrane-bound particles generated by virtually all cells. Here we show that RAW 264.7 macrophage cell-derived MVs can be used as vectors to deliver dexamethasone (named MV-DEX) targeting inflamed kidney efficiently. Methods: macrophages were incubated with and then MV-DEX was isolated from...
Significance Statement AKI is a frequent clinical problem without definitive therapies. We developed an efficient RNAi therapy against by engineering red blood cell-derived extracellular vesicles (REVs) with targeting peptides and therapeutic siRNAs. REVs targeted Kim-1–binding peptide LTH efficiently delivered P65 Snai1 siRNAs to the injured tubules, leading reduced expression of P-p65 Snai1. Dual suppression inhibited renal inflammation fibrosis in mice subjected ischemia/reperfusion...
: Cisplatin nephrotoxicity is an important cause of acute kidney injury (AKI), limiting cisplatin application in cancer therapy. Growing evidence has suggested that genome instability, telomeric dysfunction, and DNA damage were involved the tubular epithelial cells (TECs) cisplatin-induced AKI (cAKI). However, exact mechanism largely unknown.
Tubular epithelial cells (TECs) exposed to hypoxia incite tubulointerstitial inflammation (TII), while the exact mechanism is unclear. In this study, we identified that evoked tubule injury as evidenced by tubular hypoxia-inducible factor-1α and kidney molecule-1 (KIM-1) expression renal small extracellular vesicle (sEV) production was increased with development of TII after ischemia-reperfusion (IRI). Intriguingly, KIM-1-positive tubules were surrounded macrophages co-localized sEVs. vitro,...
Peritubular capillaries (PTCs) are closely related to renal tubules in structure and function, both pivotal regulators the development progression of acute kidney injury (AKI). However, mechanisms that underlie interaction between PTCs during AKI remain unclear. Here we explored a new mode tubulovascular crosstalk mediated by small extracellular vesicles (sEV) after AKI. In response ischemia/reperfusion (I/R) injury, endothelial proliferation tubular expression vascular growth factor-A...
Abstract Staphylococcus aureus ( S. ) remains a leading cause of bacterial infections. However, eradication infections with common antibiotics is increasingly difficult due to outbreaks drug resistance. Therefore, new antibiotic classes and antibacterial strategies are urgently in demand. Herein, it shown that an adamantane‐peptide conjugate, upon dephosphorylation by alkaline phosphatase (ALP) constitutively expressed on , generates fibrous assemblies situ combat infection. By attaching...
Oxygen homeostasis disturbances play a critical role in the pathogenesis of acute kidney injury (AKI). The transcription factor hypoxia-inducible factor-1 (HIF-1) is master regulator adaptive responses to hypoxia. Aside from posttranslational hydroxylation, mechanism HIF-1 regulation AKI remains largely unclear. In this study, HIF-α was investigated. We found that tubular HIF-1α expression significantly increased at transcriptional level ischemia-reperfusion-, unilateral ureteral...
Tubules injury and immune cell activation are the common pathogenic mechanisms in acute kidney (AKI). However, exact modes of following tubule damage not fully understood. Here we uncovered that release cytoplasmic spliceosome associated protein 130 (SAP130) from damaged tubular cells mediated necroinflammation by triggering macrophage via miRNA-219c(miR-219c)/Mincle-dependent mechanism unilateral ureteral obstruction (UUO) cisplatin-induced AKI mouse models, patients with necrosis (ATN). In...
(1) Background: The rational allocation of limited medical resources is the premise safeguarding public health. Especially since outbreak COVID-19, evolution dynamics and spatial mismatch have been a focal frontier issue in academic discussions. (2) Methods: Based on competitive state model index, this paper uses GIS Geodetector analysis methods three typical indicators hospitals, doctors, beds to conduct an empirical study evolutionary characteristics degree geographic pattern health China...
Abstract Background/Aims Diabetic nephropathy (DN) is one of the main causes end-stage kidney disease worldwide. Emerging studies have suggested that its pathogenesis distinct from nondiabetic renal diseases in many aspects. However, it still lacks a comprehensive understanding unique molecular mechanism DN. Methods A total 255 Affymetrix U133 microarray datasets (Affymetrix, Santa Calra, CA, USA) human glomerular and tubulointerstitial tissues were collected. The 22 215 identifiers shared...
Foot process effacement is an important feature of early diabetic nephropathy (DN), which closely related to the development albuminuria. Under certain nephrotic conditions, integrity and function glomerular slit diaphragm (SD) structure were impaired replaced by tight junction (TJ) structure, resulting in so-called SD-TJ transition, could partially explain foot processes at molecular level. However, mechanism underlying transition has not been described DN. Here, we demonstrated that...
Renal ischemia/reperfusion (I/R) injury is a major cause of acute kidney (AKI), characterized by tubulointerstitial inflammation. Currently, progress in developing effective therapies to prevent or ameliorate AKI anti-inflammation remains slow. Emerging studies have suggested that NLRP3 (the NOD-, LRR- and pyrin domain-containing 3) inflammasome plays key role wide spectrum disease models including I/R injury. In this study, we investigated the renal protective effects A68930, specific...
Urinary sediment messenger RNAs (mRNAs) have been shown as novel biomarkers of kidney disease. We aimed to identify targeted urinary mRNAs in diabetic nephropathy (DN) based on bioinformatics analysis and clinical validation.Microarray studies DN were searched the GEO database Nephroseq platform. Gene modules negatively correlated with estimated glomerular filtration rate (eGFR) identified by informatics methods. Hub genes screened within selected modules. In validation cohorts, a...
Previous studies suggest that patients with nephrolithiasis exhibit dysbiosis in their gut microbiota, but those were conducted calcium oxalate stone patients. We aimed to explore the association of microbiota and biochemical features renal uric acid (UAS) a Chinese population identify related bacteria may affect pathopoiesis UAS. A case-control study 117 UAS, 123 gout, 135 healthy controls included from January 2014 October 2020. For each subject, data on demographics, parameters blood...
Objective: The roles of long non-coding RNAs (lncRNAs) in the diagnosis clear cell renal carcinoma (ccRCC) are still not well-defined. We aimed to identify differentially expressed lncRNAs and mRNAs plasma ccRCC patients health controls systematically. Methods: Expression profile healthy was analyzed based on microarray assay. Gene ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway-based approaches were used investigate biological function signaling pathways mediated by mRNAs....
Direct tubular injury caused by several medications, especially chemotherapeutic drugs, is a common cause of AKI. Inhibition or loss cyclin-dependent kinase 12 (CDK12) triggers transcriptional elongation defect that results in deficiencies DNA damage repair, producing genomic instability variety cancers. Notably, 10-25% individuals developed AKI after treatment with CDK12 inhibitor, and the potential mechanism not well understood. Here, we found was downregulated renal epithelial cells both...
Abstract Background and Aims Acute kidney injury (AKI) is a public health problem that seriously endangers human health. The exact pathogenesis of AKI has not been fully elucidated. Previous studies have shown the immune system plays key role in inflammatory response acute phase, especially innate immunity. Recent discoveries highlighted neutrophils, particular neutrophil extracellular traps (NETs), central roles occurrence development AKI. However, specific mechanism NETs different...