A5054333438- Renal Diseases and Glomerulopathies
- Complement system in diseases
- Gut microbiota and health
- Proteoglycans and glycosaminoglycans research
- Probiotics and Fermented Foods
- Cell Adhesion Molecules Research
- Tissue Engineering and Regenerative Medicine
- Renal Transplantation Outcomes and Treatments
- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- Platelet Disorders and Treatments
- Chemokine receptors and signaling
- Microbial Inactivation Methods
- Extracellular vesicles in disease
- Cancer Genomics and Diagnostics
- Renal and related cancers
- Adenosine and Purinergic Signaling
- Single-cell and spatial transcriptomics
- Digestive system and related health
- Microbial Metabolites in Food Biotechnology
Newcastle University
2019-2021
Abstract The thick mucus layer of the gut provides a barrier to infiltration underlying epithelia by both normal microbiota and enteric pathogens. Some members utilise mucin glycoproteins as nutrient source, but detailed understanding mechanisms used breakdown these complex macromolecules is lacking. Here we describe discovery characterisation endo-acting enzymes from prominent mucin-degrading bacteria that target polyLacNAc structures within oligosaccharide side chains animal human mucins....
Background and objectives Membranoproliferative GN C3 glomerulopathy are rare overlapping disorders associated with dysregulation of the alternative complement pathway. Specific etiologic data for pediatric membranoproliferative GN/C3 lacking, outcome based on retrospective studies without data. Design, setting, participants, & measurements A total 80 prevalent patients underwent detailed phenotyping long-term follow-up within National Registry Rare Kidney Diseases (RaDaR). Risk factors...
In fibrotic diseases, myofibroblasts derive from a range of cell types including endothelial-to-mesenchymal transition (EndMT). Increasing evidence suggests that miRNAs are key regulators in biological processes but their profile is relatively understudied EndMT. human umbilical vein endothelial cells (HUVEC), EndMT was induced by treatment with TGFβ2 and IL1β. A significant decrease markers such as VE-cadherin, CD31 an increase mesenchymal fibronectin were observed. parallel, miRNA...
Atypical hemolytic uremic syndrome (aHUS) is frequently associated in humans with loss-of-function mutations complement-regulating proteins or gain-of-function complement-activating proteins. Thus, aHUS provides an archetypal complement-mediated disease which to model new therapeutic strategies and treatments. Herein, we show that, when transferred mice, aHUS-associated change (D1115N) the complement-activation protein C3 results aHUS. Homozygous p.D1115N (C3KI) mice developed spontaneous...
Chemokine receptor CCR7 is implicated in the metastasis of breast cancer to lymph nodes. function dependent upon their binding both cell-surface heparan sulphate (HS) and specific receptors; thus, role HS CCR7-mediated node was investigated by creating a non-HS chemokine CCL21 (mut-CCL21). Mut-CCL21 (Δ103–134) induced leukocyte chemotaxis diffusion gradients but did not stimulate trans-endothelial migration PBMCs (p < 0.001) 4T1-Luc cells 0.01). Furthermore, effect heparin on chemotactic...
Abstract The human gut microbiota (HGM) are closely associated with health, development and disease. thick intestinal mucus layer, especially in the colon, is key barrier between contents of lumen epithelial cells, providing protection against infiltration by as well potential pathogens. upper layer colonic a niche for subset which utilise mucin glycoproteins nutrient source grazing appears to play role maintaining function community stability. Despite importance breakdown mechanisms...
Abstract Background Conventional cellular phenotyping of intestinal cell populations by multi-parameter fluorescence cytometry is reliant on collection fresh tissue for immediate enzymatic or mechanical disaggregation, cryopreservation samples. These factors limit widescale use research, increase cost, and time sample preparation. Due to disaggregation suspension does not provide data regarding spatial localisation cells in tissue. Formalin fixed paraffin embedded (FFPE) widely collected at...