Machine learning classifiers are an effective approach to detecting glaucomatous fundus images based on optic disc topographic features making it a straightforward and approach.

Purpose: To investigate the morphological and functional outcome of refractory large macular hole (MH) with autologous neurosensory retinal free flap transplantation. Methods: This case series enrolled 10 patients suffering from MH at Kaohsiung Medical University Hospital, Kaohsiung, Taiwan. All eyes underwent pars plana vitrectomy, a 1.5 to 2-MH diameter was harvested. We used an adhesive agent such as whole blood or Viscoat assist stabilization then use tamponade silicone oil vitreous...

Myopic foveoschisis occurs in 9-34% of highly myopic eyes with posterior staphyloma. The pathogenesis is still not fully understood. But the relative inflexibility inner retina and a tangential traction induced inward force staphyloma are possible mechanisms. Conventional internal limiting membrane (ILM) peeling generally yields good results. However, postoperative full-thickness macular hole happens 13-28% cases. Therefore, this study describes modified ILM technique named 'ILM...

Improper use of mitomycin-C in ocular medication may result damage to corneal cells. In this study, the toxic effects on cultured porcine keratocytes and endothelial cells were estimated by MTT, 3H-thymidine uptake cellular counting assay methods. It was found that caused a dose-dependent effect Both treated with at concentration ranging from 100, 10, 1, 0.1 0.01 μg/ml for 3 min, 5 min or 100 min. The 50% inhibitory dose (ID50) as measured MTT 0.40, 0.18, 0.16 mg/ml 0.27, 0.15, 0.14 mg/ml,...

Hypoxia is the most important stimulus leading to up-regulation of vascular endothelial growth factor (VEGF) in retina via elevation hypoxia-inducible factors-1α (HIF-1α) protein. The purpose this study was test effects melatonin on expression VEGF and HIF-1α cultured human retinal pigment epithelial (RPE) cells under normoxia hypoxia.An vitro RPE cell hypoxia model established by placing 1% oxygen pressure or adding cobalt chloride (CoCl2 ) culture medium. conditioned media were collected...

In this study, the various antiglaucoma drugs including betaxolol, timolol, levobunolol, carteolol, brimonidine, dipivefrin, dorzolamide, brinzolamide, latanoprost, unoprostone, and pilocarpine were used to investigate effects of cellular cytotoxicity in cultured bovine corneal endothelial cells. After exposure three dilutions, 1/100, 1/1,000, 1/10,000, for 100 minutes, cells estimated based on release assay lactate dehydrogenase (LDH) enzyme. It was found that LDH significantly released...

Purpose: To identify the association between sleep apnea (SA) and central serous chorioretinopathy (CSC). Methods: In this nationwide population-based study using Taiwan National Health Insurance Database, we enrolled adult patients with a diagnosis of SA matched each patient to 30 age- gender-matched control subjects without any diagnosis. Using Poisson regression analyses, incidence rate CSC was compared subjects. Results: A total 10,753 322,590 were identified. After adjusting for age,...

Purpose: Latanoprost reduces intraocular pressure mainly by enhancing uveoscleral outflow that may be involved in the decreased of extracellular matrixes such as collagens. However, effect latanoprost on corneal stromal cells is not well understood. In current study, we investigated changes cultured porcine upon exposure to latanoprost. Methods: Porcine were acquired from primary culture and maintained fetal bovine serum–containing medium. Cells estimated...

Purpose . To investigate the functional and anatomical outcome of 0.7 mg dexamethasone (Ozurdex) intravitreal implant (IVD) in eyes with long-term macular edema after epiretinal membrane removal. Methods We enrolled 40 persistent at least 12 months Twenty IVD group received other 20 were control group. The main measures change best-corrected visual acuity (BCVA) central foveal thickness (CFT). Results For group, mean BCVA improved by 3.45 lines to 0.47 logMAR one month IVD. However, only...

The aim of this study was to estimate the effects various antiglaucoma drugs including betaxolol, timolol, levobunolol, brimonidine, carteolol, dipivefrin, dorzolamide, brinzolamide, latanoprost, unoprostone, and pilocarpine on intracellular free Ca 2+ ([Ca ] i ) mobility in cultured bovine corneal endothelial cells. Various were diluted from original concentrations 1/100, 1/1,000, 1/10,000. [Ca studied by spectrofluorophotometry after loading with ester fura‐2 (fura‐2/AM). It found that...

Many studies have been conducted to elucidate the molecular consequences of ultraviolet irradiation, whereas little is known about effect infrared radiation on ocular disease. Heat generated as a consequence in addition photons, and heat shock widely considered be an environmental stressor. Our previous study has demonstrated that (42 °C) induces apoptosis Statens Seruminstitut Rabbit Cornea (SIRC) cells. However, biological mild (39 °C), which does not induce apoptosis, SIRC cells studied...

Purpose: Repeated subconjunctival injections with 5-fluorouracil (5-FU) after trabeculectomy are used in glaucoma patients for the inhibition of overproliferation wound site. Thus, a certain amount drug may penetrate into epithelial layer, where it causes toxicity to corneal cells. The aim this study was evaluate toxic effects 5-FU and mechanisms drug-induced apoptosis cultured Methods: Cellular damage caspase pathway were estimated 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium...

To evaluate the long-term anatomic and functional outcomes of autologous neurosensory retinal free flap transplantation (ART) for patients with refractory large macular hole (MH). Retrospective interventional case series. We reviewed 9 who underwent ART their MH. In this extended follow-up study, postoperative assessment including spectral-domain optical coherence tomography best-corrected visual acuity (BCVA) were recorded at 12, 15, 18, 21, 24 months after surgery. The all appeared...

Mitomycin-C has recently become an adjunct medication for inhibition of fibroblast proliferation in glaucoma filtering procedures. Prolonged postoperative ocular hypotony been a frequent complication trabeculectomy with mitomycin-C. In order to characterize the mechanism, we compared toxic effects mitomycin-C on cultured rabbit ciliary process cells and trabecular meshwork cells. The results indicate that more marked effect 3H-thymidine uptake than at concentrations ranging from 10-1 10-5...

Over contraction of vascular smooth muscle may result in ischemia to ocular neuronal cells and deteriorate the glaucoma. The purpose this study was investigate inhibitory effects various commercial antiglaucoma drugs including brimonidine, dipivefrin, betaxolol, timolol, levobunolol, carteolol, brinzolamide, dorzolamide, unoprostone, latanoprost, pilocarpine, preservative benzalkonium chloride on endothelin-1(ET-1) KCl-induced increase intracellular free Ca2+ ([Ca2+]i) cultured rat A7r5...

Abstract Extracellular matrix (ECM) plays an active and complex role in regulating cellular behaviors, including proliferation adhesion. This study aimed at delineating the adhesion‐induced signaling profiles cultured human retinal pigment epithelium (RPE) cells investigating antiadhesion effect of antiproliferative drugs this context. RPE R‐50 grown on various ECM molecules, such as type I IV collagens, fibronectin, laminin, were used for adhesion assay examining phosphorylation mediators...

A 39-year-old male with open angle glaucoma in both eyes visited our clinic. The intraocular pressure (IOP) of fluctuated between 15 mmHg and 25 mmHg. best corrected visual acuity (BCVA) was 20/20 the right eye 20/100 left eye. He underwent trabeculectomy an adjunctive agent (mitomycin C; concentration, 0.2 mg/mL) smoothly on After removal releasable sutures, filtering bleb prominent over superior limbus for 3 consecutive clock hours IOP about 4-5 Seidel test result negative, anterior...

Repeated subconjunctival injections with 5-fluorouacil (5-FU) after trabeculectomy are widely used in glaucoma patients for the inhibition of excess scar formation wound site. The aim this study was to evaluate toxic effects 5-FU and mechanisms drug-induced apoptosis cultured porcine corneal endothelial cells. Cellular damage caspase pathway were estimated a MTT assay. apoptotic characteristics detected flow cytometry, TUNEL test Western blotting. results indicated that cells time-...

It has been reported that β-adrenergic receptors are localized in the corneal endothelial cells. In this study, change of cellular signal transduction, such as intracellular calcium and cAMP, was determined with pure adrenergic agonists commercial antiglaucoma agents. The cultured porcine cells inhibited by 10 μM isoproterenol norepinephrine, but enhanced propranolol 50 mM KCl. case phenylephrine, mobility did not alter significantly. Verapamil, at μM, decreased concentration. presence...

Over releasing of glutamate and cellular calcium influx always results in neuronal death. In the present study, we investigated various commercial antiglaucoma drugs including timolol (0.58 μM to 58 μM), betaxolol (1.62 162 carteolol (6.8 680 pilocarpine (4.08 408 latanoprost (0.01 1.1 dorzolamide (6.16 616 brinzolamide (2.6 260 brimonidine (0.68 68 dipivefrin (0.28 28 μM) preservative benzalkonium chloride on their effects inhibit glutamate-induced intracellular free Ca2+ ([Ca2+]i) increase...