A5051735788- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- MicroRNA in disease regulation
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Poxvirus research and outbreaks
- Viral Infections and Immunology Research
- Plant Virus Research Studies
- COVID-19 Clinical Research Studies
- DNA and Nucleic Acid Chemistry
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Virology and Viral Diseases
- Acoustic Wave Resonator Technologies
- Bacillus and Francisella bacterial research
- Biosensors and Analytical Detection
- Herpesvirus Infections and Treatments
- SARS-CoV-2 detection and testing
- Insect symbiosis and bacterial influences
- Viral Infections and Outbreaks Research
- Plant Pathogenic Bacteria Studies
- Micro and Nano Robotics
Academy of Military Medical Sciences
2023-2025
Southern Medical University
2021-2025
Institute of Microbiology
2021-2024
Institute of Microbiology
2024
Wuhan Institute of Virology
2022
China Agricultural University
2016-2021
Astroviruses are recognized as a leading cause of gastroenteritis in humans and animals. They also associated with extra-intestinal diseases, such hepatitis ducklings, nephritis chickens, encephalitis cattle. In February 2017, fatal infection goslings characterized by visceral urate deposition was reported the Shandong province, China. Our systematic investigation led to isolation an astrovirus, designated AAstV/Goose/CHN/2017/SD01, similar disease reproduced experimental healthy goslings,...
Early tumor diagnosis is crucial to successful treatment. Earlier studies have shown that microRNA a biomarker for early diagnosis. The development of highly sensitive miRNA detection methods, especially in living cells, plays an indispensable role and treatment tumor. Although the catalytic hairpin assembly (CHA)-based analysis strategy commonly used disease diagnosis, further application CHA hindered due its low amplification efficiency recognition contrast. To address these limitations,...
As an important disease biomarker, the development of sensitive detection strategies for miRNA, especially intracellular miRNA imaging strategies, is helpful early diagnosis diseases, pathological research, and drug development. Hybridization chain reaction (HCR) widely used analysis because its high specificity lack biological enzymes. However, classic HCR exhibits linear amplification with low efficiency, limiting use rapid trace in living cells. To address this problem, we proposed a...
Accurate and specific imaging of low-abundance microRNA (miRNA) in living cells is extremely important for disease diagnosis monitoring progression. DNA nanomotors have shown great potential molecules interest cells. However, inappropriate driving forces complex design operation procedures hindered their further application. Here, we proposed an endogenous enzyme-powered nanomotor (EEPDN), which employs APE1 enzyme as fuel to execute repetitive cycles motion miRNA The whole motor system...
Zika virus (ZIKV) infection during pregnancy threatens and fetal health. However, the infectivity pathological effects of ZIKV on placental trophoblast progenitor cells in early human embryos remain largely unknown. Here, using stem (hTSCs), we demonstrated that hTSCs were permissive to infection, resistance increased with hTSC differentiation. Combining gene knockout transcriptome analysis, intrinsic expression AXL TIM-1, absence potent interferon (IFN)-stimulated genes (ISGs) IFNs...
ABSTRACT The live-attenuated yellow fever (YF) vaccine strain 17D (referred to as YF-17D) is the only commercially available against YF. Recent virus (YFV) isolates of South American genotype I (SAI) showed reduced sensitivity sera from individuals vaccinated with YF-17D. However, underlying mechanisms remain inadequately elucidated. Here we conducted a screening panel amino acid changes prevalent in domain II envelope protein (EDII) SAI YFV isolates. Each mutation was introduced into...
Nanoflares (NFs) are extensively used for the analysis of biomarkers within living cells. However, severe false positives and low reaction efficiency kinetics have restricted further application NF. To address these issues, we developed an aptamer-modified DNA tetrahedron-assembled 3D nucleic acid nanoshell-mediated nanoflares (Ap-TDN-NF) highly sensitive accurate miRNA in Compared to traditional NF system, Ap-TDN-NF system exhibits enhanced stability improved cellular uptake due...
Development of versatile sensing methods for sensitive and specific detection clinically relevant nucleic acids proteins is great value disease monitoring diagnosis. In this work, we propose a novel isothermal Self-primer EXPonential Amplification Reaction (SPEXPAR) strategy based on rationally engineered structure-switchable Metastable Hairpin template (MH-template). The MH-template initially keeps inactive with its self-primer overhanging part target recognition region to inhibit...
SARS-CoV-2 has evolved into a panel of variants concern (VOCs) and constituted sustained threat to global health. The wildtype (WT) isolates fail infect mice, while the Beta variant, one VOCs, acquired capability standard laboratory raising spreading risk from humans mice. However, infectivity pathogenicity other VOCs in mice remain not fully understood. In this study, we systematically investigated three Alpha, Beta, Delta, comparison with two well-understood mouse-adapted strains, MASCp6...
A domino-like localized cascade toehold assembly amplification-based DNA nanowire was proposed for microRNA imaging in living cells.
Outbreaks of Tembusu virus (TMUV) infection have caused huge economic losses in the production domestic waterfowl since was first recognized China 2010. To control TMUV infection, a live-attenuated vaccine candidate developed our previous study, but mechanisms virulence attenuation are not fully understood. Here, we found that Thr-to-Lys substitution at E367 is crucial determinant ducks. We demonstrated T367K mutation attenuates through reducing viral replication blood, brain, heart...
Summary The emergence and global spread of SARS-CoV-2 variants has caused concerns about increased virulence decreased efficacy medical countermeasures. Herein, we demonstrate that the newly emerged 501Y.V2 variant (also called beta variant) is capable infecting standard laboratory mice after intranasal instillation, unlike earlier isolates. However, infectivity in demonstrated may not occur nature; indeed, there no evidence natural transmission to date. Most interestingly, was quickly...
Virus spillover remains a major challenge to public health. A panel of SARS-CoV-2-related coronaviruses have been identified in pangolins, while the infectivity and pathogenicity these pangolin-origin (pCoV) humans remain largely unknown. Herein, we comprehensively characterized recent pCoV isolate (pCoV-GD01) human cells tracheal epithelium organoids established animal models comparison with SARS-CoV-2. pCoV-GD01 showed similar SARS-CoV-2 organoids. Remarkably, intranasal inoculation caused...
Abstract In June 2016, a disease characterised by intestinal haemorrhage with mortality rate of approximately 5% was observed in duck farm Shandong province, China. Here, we report the isolation and characterisation reovirus from tissue samples inoculating embryos embryo fibroblasts (DEF). The isolate replicated DEF Vero cells formed syncytia. Sequence analysis revealed that viral genome 23,434 nt length typical structure organization, consisting 10 dsRNA segments ranging 3998 (L1) to 1190...
Tembusu virus (TMUV) associated disease is a growing cause of egg production decrease and encephalitis in domestic waterfowl, with expanding distribution. In previous studies, TMUV isolates were phylogenetically classified into two genetic lineages different clusters varied pathogenicity. However, little known about the phenotypic virulence characteristics cluster 3 within duck lineage. this study, etiological agent causing drop laying chicken farm southern China was investigated isolated...
The recent worldwide outbreaks of mpox prioritize the development a safe and effective mRNA vaccine. contemporary virus (MPXV) exhibits changing virological epidemiological features, notably affecting populations already vulnerable to human immunodeficiency (HIV). Herein, we profile immunogenicity AR-MPXV5, penta-component vaccine targeting five specific proteins (M1R, E8L, A29L, A35R, B6R) from representative MPXV clade II strain, in both naive simian (SIV)-infected nonhuman primates....