A5100369397- Cytomegalovirus and herpesvirus research
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Herpesvirus Infections and Treatments
- Immunotherapy and Immune Responses
- Cervical Cancer and HPV Research
- Poxvirus research and outbreaks
- Viral Infections and Immunology Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Immune Response and Inflammation
- Influenza Virus Research Studies
- Sperm and Testicular Function
- Toxin Mechanisms and Immunotoxins
- Healthcare Systems and Public Health
- Parvovirus B19 Infection Studies
- Bacillus and Francisella bacterial research
- Peripheral Neuropathies and Disorders
- Thermal Regulation in Medicine
- Breastfeeding Practices and Influences
- T-cell and B-cell Immunology
- Reproductive Health and Technologies
- Renal Transplantation Outcomes and Treatments
- Endometrial and Cervical Cancer Treatments
Chinese Academy of Medical Sciences & Peking Union Medical College
2020-2025
Institute of Laboratory Animal Science
2022-2024
First Affiliated Hospital of Anhui Medical University
2024
Anhui Medical University
2021-2024
Blood Center of Zhejiang Province
2021-2024
Academy of Medical Sciences
2024
Nanomed (Brazil)
2023
National University of Singapore
2023
Peking Union Medical College Hospital
2023
Qilu Hospital of Shandong University
2023
Persistence of hepatitis B virus (HBV) infection requires covalently closed circular (ccc)DNA formation and amplification, which can occur via intracellular recycling the viral polymerase-linked relaxed (rc) DNA genomes present in virions. Here we reveal a fundamental difference between HBV related duck (DHBV) mechanism. Direct comparison DHBV cccDNA amplification cross-species transfection experiments showed that, same human cell background, but not rcDNA converts efficiently into cccDNA....
Herpes simplex virus type 1 (HSV-1) is a very concerning pathogen due to its ability persist in the host’s nervous system and continuously interfere with immune system, which complicates treatment. Therefore, development of an effective HSV-1 vaccine crucial. In this study, we focused on mutant strain, M6, includes several deleted genes associated viral infection virulence latent function, explored macrophages immunological characteristics. The study found that both attenuated strain M6...
To understand knowledge about, and acceptability of, cervical cancer screening HPV vaccines among medical students; to explore potential factors that influence their in China.We conducted a survey students at six universities across southwest China using 58-item questionnaire regarding perceptions of HPV, cancer, vaccines.We surveyed 1878 with mean age 20.8 years (standard deviation: 1.3 years). Of these, 48.8% 80.1% believed can be prevented by respectively, while 60.2% 71.2% would like...
Abstract Background Hepatitis B virus (HBV) infection continues to be an escalating global health problem. Feasible and effective animal models for HBV are the prerequisite developing novel therapies this disease. The tree shrew ( Tupaia ) is a small species evolutionary closely related humans, thus permissive certain human viral pathogens. Whether shrews could chronically infected with in vivo has been controversial decades. Most published research reported on adult shrews, only numbers of...
Coxsackievirus A10 (CV-A10) is one of the etiological agents associated with hand, foot and mouth disease (HFMD) also causes a variety illnesses in humans, including pneumonia, myocarditis. Different people, particularly young children, may have different immunological responses to infection. Current CV-A10 infection animal models provide only rudimentary understanding pathogenesis effects this virus. The characteristics infection, replication, shedding humans remain unknown. In study,...
Proofreading deficiencies of hepatitis B virus polymerase result in frequent DNA mutations the genome. Here, we performed sequencing analysis gene to assess its association with postoperative survival 92 patients HBV-related hepatocellular carcinoma by using Kaplan–Meier method. The 2525, 2733, 2738, 2768, 2946, 3063, 3066, 3109, 31, 529, 735, 939, 1078, 1137, 1383, 1461, 1485, 1544, and 1613 mutation sites were identified as being associated HCC outcomes log-rank test. After adjusting for...
Kidney is considered to be one of the dose-limiting organs in peptide receptor radionuclide therapy (PRRT). Amino acid cocktail infusion has been applied reduce renal absorbed dose by inhibiting proximal tubular reabsorption radiopeptide. An Evans blue-modified 177 Lu-labeled octreotate ( Lu-DOTA-EB-TATE) an extended circulation blood, which may make amino unnecessary. The aim this study was evaluate safety, biodistribution, and dosimetry Lu-DOTA-EB-TATE with without infusion.Ten patients...
The recent worldwide outbreaks of mpox prioritize the development a safe and effective mRNA vaccine. contemporary virus (MPXV) exhibits changing virological epidemiological features, notably affecting populations already vulnerable to human immunodeficiency (HIV). Herein, we profile immunogenicity AR-MPXV5, penta-component vaccine targeting five specific proteins (M1R, E8L, A29L, A35R, B6R) from representative MPXV clade II strain, in both naive simian (SIV)-infected nonhuman primates....
Objective: We constructed two DNA vaccines containing the receptor-binding domain (RBD) genes of multiple SARS-CoV-2 variants and used them in combination with inactivated a variety different protocols to explore potential novel immunization strategies against variants. Methods: Two vaccine candidates signal peptides (namely, secreted membrane peptides) RBD protein strains (Wuhan-Hu-1, B.1.351, B.1.617.2, C.37) were used. Four combinations tested, namely, Group A: three doses vaccine; B: one...
Abstract Background Nucleotide mutations in the ABO gene may reduce activity of glycosyltransferase, resulting lower levels A or B antigen expression red blood cells. Six known splice sites have been identified according to database cell immunogenetics and group terminology International Society Blood Transfusion. Here, we describe six distinct site variants individuals with subtypes. Methods The phenotype was examined using a conventional serological method. polymerase chain reaction...
HSV-2 (Herpes simplex virus type 2) is a critical viral agent that mainly causes genital herpes and life-long latent infection in the dorsal root ganglia. Gene modification via CRISPR/Cas9 Clustered regularly interspaced short palindromic repeat sequences/CRISPR associated 9) was used here to construct mutant strains through deletion of fragments RL1 (Repeat Long element 1) and/or LAT (Latency-associated Transcript) genes. The LAT-HSV-2 RL1-LAT-HSV-2 present different biological properties....
The recent emergence of COVID-19 variants has necessitated the development new vaccines that stimulate formation high levels neutralizing antibodies against S antigen variants. A strategy involves intradermal administration heterologous composed one or two doses inactivated vaccine and a booster dose with mutated S1 protein (K-S). Such improve immune efficacy by increasing antibody titers promoting specific T cell responses five RBD protein. viral challenge test B.1.617.2 (Delta) variant...
Photochemical virus inactivation technology is widely used to improve the safety of blood products. However, process by which this occurs and resulting immunogenicity treated viruses remain be elucidated. This study aimed explore effects two photochemical methods (methylene riboflavin, MP RP) on hepatitis B (HBV) immunogenicity. Inactivated HBV were incubated with PBMC from six healthy donors. Culture supernatants collected at 0, 24 72 h for analysis HBsAg HBeAg expression using ELISA....
To determine the methods for establishing an in vivo model of long-term hepatitis B virus (HBV) infection Chinese tree shrew (Tupaia belangeri chinensis).Seventy-seven neonate (1-3 days old) and 49 young adult (2 weeks to 1 year shrews were inoculated with different HBV sources (chronic (CHB) human patient serum, single or pooled; HBV-infected only; HepG2.2.15 cells' culture medium supernatant; genome-transfected supernatant) through various routes injection (subcutaneous, intraperitoneal,...
Herpes simplex virus type 1 (HSV-1), an α subgroup member of the human herpesvirus family, infects cells via binding its various envelope glycoproteins to cellular membrane receptors, one which is herpes entry mediator (HVEM), expressed on dendritic cells. Here, HVEM gene-deficient mice were used investigate immunologic effect elicited by HSV-1 infection Dendritic expressing surface marker CD11c showed abnormal biological phenotype, including altered transcription immune signaling molecules...
Emerging viruses in the blood of healthy/qualified donors can seriously affect transfusion safety. However, virus characteristics different healthy and components are still not fully understood.
<title>Abstract</title> The recent worldwide outbreaks of mpox (monkeypox) prioritize the development a safe and effective mRNA vaccine. contemporary virus (MPXV) showed changing virological epidemiological features, notably affecting populations already vulnerable to human immunodeficiency (HIV). Herein, we profiled immunogenicity protection AR-MPXV5, novel penta-component vaccine targeting five specific proteins (M1R, E8L, A29L, A35R, B6R) from representative MPXV clade II strain, in both...
Abstract Background The presence of predominantly headless sperm in semen is a hallmark acephalic spermatozoa syndrome, which primarily caused by gene mutations humans. Purpose To identify genetic causes for syndrome. Methods Polymerase chain reaction and Sanger sequencing were performed to define SUN5 PMFBP1 . Whole‐exome was on the patients pathogenic infertility. Western blotting immunofluorescence analysis detected expression level localization CEP250. Co‐immunoprecipitation...
Antiretroviral therapy is capable of inhibiting HIV replication, but it fails to completely achieve a cure due persistence. The commonly used approach the “shock and kill” strategy, which employs latency-reversing agents trigger viral reactivation boost cellular immunity. Finding appropriate drug combination for strategy would greatly facilitate clinical trials. toll-like receptor (TLR) 7 agonist GS–9620 nicotinamide (NAM) are reported as potential agents. Herein, we found absence when...
Herpes simplex virus type 2 (HSV2), a pathogen that causes genital herpes lesions, interferes with the host immune system via various known and unknown mechanisms. This has been used to study viral antigenic composition. Convalescent serum from HSV2-infected patients was identify antigens 2-D protein electrophoresis immunoblotting. The predominantly recognized several capsid scaffold proteins encoded by gene UL26.5, mainly ICP35. primarily reported function temporarily in assembly but is not...