A5069441124- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Electrochemical sensors and biosensors
- Biosensors and Analytical Detection
- Machine Learning in Bioinformatics
- Epigenetics and DNA Methylation
- Computational Drug Discovery Methods
- Electrochemical Analysis and Applications
- Bioinformatics and Genomic Networks
- Metal-Organic Frameworks: Synthesis and Applications
- RNA modifications and cancer
- DNA and Nucleic Acid Chemistry
- Analytical Chemistry and Sensors
- Advanced Nanomaterials in Catalysis
- RNA and protein synthesis mechanisms
- Conducting polymers and applications
- Nanoplatforms for cancer theranostics
- Molecular Sensors and Ion Detection
- Advanced Biosensing Techniques and Applications
- Extracellular vesicles in disease
- Protein Structure and Dynamics
- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- Genomics and Phylogenetic Studies
Sun Yat-sen University
2016-2025
China National Offshore Oil Corporation (China)
2021
State Administration of Traditional Chinese Medicine of the People's Republic of China
2020
Hefei University of Technology
2019
State Council of the People's Republic of China
2018
China Nonferrous Metal Mining (China)
2017
Zhongkai University of Agriculture and Engineering
2010
Arizona State University
2006
Nanjing University
2001-2006
Purdue University West Lafayette
2006
Abstract Cuproptosis, a newly identified form of regulated cell death that is copper‐dependent, offers great opportunities for exploring the use copper‐based nanomaterials inducing cuproptosis cancer treatment. Here, glucose oxidase (GOx)‐engineered nonporous copper(I) 1,2,4‐triazolate ([Cu(tz)]) coordination polymer (CP) nanoplatform, denoted as GOx@[Cu(tz)], starvation‐augmented and photodynamic synergistic therapy developed. Importantly, catalytic activity GOx shielded in scaffold but can...
Specific and sensitive detection of microRNAs continues to encounter significant challenges, especially in the development rapid efficient isothermal amplification strategies for point-of-care settings. The exponential reaction (EXPAR) has garnered attention owing its simplicity signals within a short period. However, substantial loss efficiency, difficulty distinguishing closely related homologous sequences, adapting designed templates other targets seriously hamper practical application...
Enzymatic sensors possess high selectivity but suffer from some limitations such as instability, complicated modified procedure, and critical environmental factors, which stimulate the development of more sensitive stable nonenzymatic electrochemical sensors. Herein, a novel sensor is proposed based on new zinc porphyrin–fullerene (C60) derivative (ZnP–C60), was designed synthesized according to conformational calculations electronic structures two typical ZnP–C60 derivatives para-ZnP–C60...
Abstract Gene silencing holds promise for cancer therapeutics because of its potential to inhibit genes involved in tumor development. However, gene is still restricted by limited efficacy and safety. Nanoscale coordination polymers (CPs) emerge as promising nanocarriers delivery, but their responsiveness therapeutic properties have rarely been explored simultaneously. Here, multifunctional ultrathin 2D nanosheets Cu(I) 1,2,4‐triazolate CP with a thickness 4.5 ± 0.8 nm are synthesized using...
Imaging of tumor-associated microRNAs (miRNAs) can provide abundant information for cancer diagnosis, whereas the occurrence trace amounts miRNAs in normal cells inevitably causes an undesired false-positive signal discrimination during miRNA imaging. In this study, we propose a dual-locked (D-locked) platform consisting enzyme/miRNA-D-locked DNAzyme sensor and honeycomb MnO2 nanosponge (hMNS) nanocarrier highly specific cell For proof-of-concept demonstration, apurinic/apyrimidinic...
Sensitive and specific imaging of microRNA (miRNA) in living cells is great value for disease diagnosis monitoring. Hybridization chain reaction (HCR) DNAzyme-based methods have been considered as powerful tools miRNA detection, with low efficient intracellular delivery limited amplification efficiency. Herein, we propose a Hairpins@MnO2 nanosystem enzyme-free exponential imaging. The based on the synergistic cross-activation between HCR DNAzymes. MnO2 nanosheets were employed carrier three...
Sensitive imaging of microRNAs (miRNAs) in living cells is significant for accurate cancer clinical diagnosis and prognosis research studies, but it challenged by inefficient intracellular delivery, instability nucleic acid probes, limited amplification efficiency. Herein, we engineered a DNAzyme-amplified cascade catalytic hairpin assembly (CHA)-based nanosystem (DCC) that overcomes these challenges improves the sensitivity. This enzyme-free based on sequential activation DNAzyme CHA. MnO2...
Early tumor diagnosis is crucial to successful treatment. Earlier studies have shown that microRNA a biomarker for early diagnosis. The development of highly sensitive miRNA detection methods, especially in living cells, plays an indispensable role and treatment tumor. Although the catalytic hairpin assembly (CHA)-based analysis strategy commonly used disease diagnosis, further application CHA hindered due its low amplification efficiency recognition contrast. To address these limitations,...
The identification of a specific tumor cell is crucial for the early diagnosis and treatment cancer. However, it remains challenge due to limited sensitivity accuracy, long response time, low contrast recent approaches. In this study, we develop dual miRNA-triggered DNA walker (DMTDW) assisted by APE1 recognition cells. miR-10b miR-155 were selected as research models. Without presence, inactive its walking strand W locked L1 L2. After are input, triggered bind L2 W-L1-L2, respectively,...
Metastasis is the leading cause of death in patients with breast cancer. Detecting high-risk cancer, including micrometastasis, at an early stage vital for customizing right and efficient therapies. In this study, we propose enzyme-free isothermal cascade amplification-based DNA logic circuit situ biomineralization nanosensor, HDNAzyme@ZIF-8, simultaneous imaging multidimensional biomarkers live cells. Taking miR-21 Ki-67 mRNA as dual detection targets achieved sensitive operations molecular...
Biosensing is vital for many areas like disease diagnosis, infectious prevention, and point-of-care monitoring. Microfluidics has been evidenced to be a powerful tool biosensing via integrating biological detection processes into palm-size chip. Based on the chip structure, microfluidics two subdivision types: open closed microfluidics, whose operation methods would diverse. In this review, we summarize fundamentals, liquid control methods, applications of separately, point out bottlenecks,...
As an important disease biomarker, the development of sensitive detection strategies for miRNA, especially intracellular miRNA imaging strategies, is helpful early diagnosis diseases, pathological research, and drug development. Hybridization chain reaction (HCR) widely used analysis because its high specificity lack biological enzymes. However, classic HCR exhibits linear amplification with low efficiency, limiting use rapid trace in living cells. To address this problem, we proposed a...
We report here for the first time on use of potentiometry ultrasensitive nanoparticle-based detection protein interactions. A silver ion-selective microelectrode is used to detect ions oxidatively released from enlarged gold nanoparticle labels in a sandwich immunoassay. Since expected largely maintain its analytical characteristics upon reducing sample volume, it anticipated that this approach may form basis bioassays with attractive limits.
A novel strategy for immunoassay and the preparation of reagentless immunosensors was proposed. This based on immobilization antigen direct electrochemistry horseradish peroxidase (HRP) that labeled to an antibody. immunosensor carcinoma antigen-125 (CA 125) determination developed. The prepared by immobilizing CA 125 with titania sol−gel a glassy carbon electrode vapor deposition method. incubation in phosphate buffer solution (PBS) including HRP-labeled antibody led formation HRP-modified...
Here we present the first report on nanoparticle-based biosensing of glycan markers diseases. The protocol relies competition between a nanocrystal (CdS)-tagged sugar and target for binding sites surface-confined lectin monitoring extent through highly sensitive electrochemical detection captured nanocrystal. This development is expected to allow decentralized carbohydrate moieties lectin-carbohydrate interactions be performed more rapidly, sensitively, inexpensively, reliably.
Accurate and specific imaging of low-abundance microRNA (miRNA) in living cells is extremely important for disease diagnosis monitoring progression. DNA nanomotors have shown great potential molecules interest cells. However, inappropriate driving forces complex design operation procedures hindered their further application. Here, we proposed an endogenous enzyme-powered nanomotor (EEPDN), which employs APE1 enzyme as fuel to execute repetitive cycles motion miRNA The whole motor system...
Metal–organic frameworks (MOFs), constructed by coordination between metal-containing nodes and organic linkers, are widely used in various fields due to the advantages of tunable pores, diverse functional sites, stable structure, multi-functionality. It should be noted that MOF-based materials play a major role glucose detection, serving as signal transducer or substrate for embedding nanoparticles/enzymes. Diabetes is one most common fast-growing diseases worldwide, whose main clinical...