A5001815218- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Molecular Sensors and Ion Detection
- HIV Research and Treatment
- Nanoplatforms for cancer theranostics
- HIV/AIDS Research and Interventions
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Sulfur Compounds in Biology
- Biosensors and Analytical Detection
- Cytomegalovirus and herpesvirus research
- Luminescence and Fluorescent Materials
- RNA and protein synthesis mechanisms
- Innovative Microfluidic and Catalytic Techniques Innovation
- Analytical Chemistry and Sensors
- Metal-Organic Frameworks: Synthesis and Applications
- MXene and MAX Phase Materials
- Respiratory viral infections research
- Ferroptosis and cancer prognosis
- Organ Donation and Transplantation
- Nanoparticle-Based Drug Delivery
- Genetic and Kidney Cyst Diseases
- Advanced Electron Microscopy Techniques and Applications
- Carbon and Quantum Dots Applications
- Bacteriophages and microbial interactions
Chinese Academy of Sciences
2017-2025
Institute of High Energy Physics
2025
China Spallation Neutron Source
2025
Sun Yat-sen University
2018-2023
National Clinical Research
2022
Xiangya Hospital Central South University
2022
Central South University
2022
Wuhan Institute of Virology
2017-2021
University of Chinese Academy of Sciences
2019-2021
University of Tennessee Health Science Center
2020
Detection and imaging RNAs in live cells is high demand. Methodology for such a purpose still challenge, particularly single RNA detection cells. In this study, type of quantum dot (QD) nanobeacon with controllable valencies was constructed by precisely conjugating the black hole quencher (BHQ1) phosphorothioate comodified DNA onto CdTe:Zn2+ QDs via one-pot hydrothermal method. The only one conjugated used to label detect low-abundance nucleic acids cells, HIV-1 were detected imaged...
Significance Influenza A virus (IAV) is one of the most important human pathogens, and it crucial to understand its life cycle develop antiviral strategies. However, IAV uncoating, an essential step in viral infection, has remained incomprehensible. Here, via construction infectious virions encapsulating quantum dots, we tracked uncoating ribonucleoprotein complex (vRNP) dynamics single virions. Our results reveal that after fusion vRNP segments are released separately into cytosol,...
Abstract Gene silencing holds promise for cancer therapeutics because of its potential to inhibit genes involved in tumor development. However, gene is still restricted by limited efficacy and safety. Nanoscale coordination polymers (CPs) emerge as promising nanocarriers delivery, but their responsiveness therapeutic properties have rarely been explored simultaneously. Here, multifunctional ultrathin 2D nanosheets Cu(I) 1,2,4‐triazolate CP with a thickness 4.5 ± 0.8 nm are synthesized using...
Imaging of tumor-associated microRNAs (miRNAs) can provide abundant information for cancer diagnosis, whereas the occurrence trace amounts miRNAs in normal cells inevitably causes an undesired false-positive signal discrimination during miRNA imaging. In this study, we propose a dual-locked (D-locked) platform consisting enzyme/miRNA-D-locked DNAzyme sensor and honeycomb MnO2 nanosponge (hMNS) nanocarrier highly specific cell For proof-of-concept demonstration, apurinic/apyrimidinic...
Macrophages are one of the major targets human immunodeficiency virus (HIV-1), but viral entry pathway remains poorly understood in these cells. Noninvasive labeling and single-virus tracking effective tools for studying entry. Here, we constructed a quantum dot (QD)-encapsulated infectious HIV-1 particle to track at single-particle level live primary macrophages. QDs were encapsulated virions by incorporating accessory protein Vpr-conjugated during assembly. With particles encapsulating...
Sensitive imaging of intracellular microRNA (miRNA) in living cells is great significance. Isothermal hybridization chain reaction (HCR)-based methods, although have been widely used to monitor low-abundance miRNA, are still subjected the challenges limited signal amplification efficiency and compromised resolution. In this work, we design a light-controlled recruitable HCR (LCR-HCR) strategy that enables us well overcome these limitations. Exosomes as delivery recruitment vehicles modified...
Sensitive and accurate imaging of intracellular-specific microRNAs (miRNAs) in situ living cells is seriously challenged by the susceptibility nucleic acid probes low dynamics hybridization reaction cellular environments. Herein, we engineer a set new metastable dumbbell (MxDPs) to overcome these key limitations concurrently boosting transfection, antidigestibility, assembly dynamics, nanostructural uniformity. The MxDPs can maintain their stability up 16 h produce uniform dense DNA...
Abstract AgSbTe 2 ‐based compounds, the most promising near‐room‐temperature thermoelectric material, so far fail to deliver n‐type performance neither theoretically nor experimentally. Its intrinsic electron‐killer defects with very low formation energy prevent conventional doping. Herein, first by building a supercompliant lattice, which is extremely soft an doping efficiency beyond Hume–Rothery rule settled phase boundary, reported. The overdosed lattice gives Young's modulus lower than...
Abstract Exploring and comprehending magnetocaloric materials with spin reorientation (SR) phase transition is of vital importance for practical applications effect (MCE). Herein, this study presents a systematic on the magnetic properties, heat transport structure, electronic structure NdNi compound. observed to undergo an SR ferromagnetic (FM) paramagnetic (PM) successively increasing temperature. Neutron powder diffraction (NPD) experiment reveals that involves rotation Nd moment from...
Using QD-encapsulated HIV particles and single-virus tracking, we revealed virus-regulated cortical actin dynamics during entry into CD4 T cells.
Abstract Single‐cell encapsulation is an emerging technology to endow cells with various functions, of which developing new applications in vivo high demand. Currently, metal–organic frameworks (MOFs) that are used as nanometric shells coat living cells, however, have not realized cell‐selective encapsulation. Here, a biocompatible and selective cell strategy based on precursor‐functionalized nucleolin aptamer situ MOF mineralization the aptamer‐identified cancer surface developed. After...
Cells in different states can release diverse types of extracellular vesicles (EVs) that participate intracellular communication or pathological processes. The identification and isolation EV subpopulations are significant to explore their physiological functions clinical value. In this study, structurally heterogeneous T-cell receptor (TCR)-CD3 EVs were proposed verified for the first time using a caliper strategy. Two CD3-targeting aptamers designed shape with an optimized probe distance...
Labeling and imaging with quantum dots (QDs) provides powerful tools to visualize viral infection in living cells. Encapsulating QDs within virions represents a novel strategy for virus labeling. Here, we developed infectious HIV-1 encapsulating through site-specific decoration of the matrix protein (MA) used them early events human primary macrophages by single-particle imaging. The MA was fused biotin acceptor peptide (BAP) tag, biotinylated, complexed streptavidin-conjugated live cells,...
Dynamic imaging of cell surface pH is extremely challenging due to the slight changes in and fast diffusion secreted acid extracellular environment. In this work, we construct a novel metal–organic framework (MOF)-shell-confined i-motif-based probe (MOFC-i) strategy that enables sensitive dynamic pH. The CY3- CY5-labeled i-motif, which hybridized via its short complementary chain with two-base mismatches, optimized for sensing at physiological After efficiently anchoring probes onto membrane...
Small extracellular vesicles (sEVs) play important roles in mediating intercellular communication and regulating biological processes. Facile sEV isolation is the essential preliminary issue for their function investigation downstream biomedical applications, while traditional methods are challenged by tedious procedures, low purity, yield, potential damage. In this work, we developed an paper-based device (sEV-IsoPD) based on a three-dimensional (3D) paper chip, which composed of porous...
We report a symmetric exponential amplification-assisted fluorescence in situ hybridization (SEXPAR-FISH) strategy for imaging intracellular-specific microRNAs (miRNAs) cells. The eliminates the risk of cell loss and miRNA leakage, low-abundant intracellular miR-27a cells can be imaged only 2 h without compromising sensitivity specificity.
A high biocompatible liposomal vehicle was used to deliver enzymes and oligonucleotide substances of the isothermal symmetric exponential amplification reaction into living cells, allowing sensitive sustained imaging microRNA in cells for more than 24 h without apoptosis.
Macrophages are one of the major targets Human Immunodeficiency virus 1 (HIV-1) and play crucial roles in viral dissemination persistence during AIDS progression. Here, we reveal dynamic podosome-mediated entry HIV-1 into macrophages. Inhibition podosomes prevented macrophages, while stimulation podosome formation promoted entry. Single-virus tracking revealed temporal spatial mechanism process. The core ring structures played complex HIV coreceptor, CCR5, was recruited to form specific...