A5100389485- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- HIV Research and Treatment
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Bacillus and Francisella bacterial research
- RNA Research and Splicing
- Advanced Biosensing Techniques and Applications
- Biotin and Related Studies
- Electrochemical sensors and biosensors
- RNA modifications and cancer
- Biosensors and Analytical Detection
- Cytomegalovirus and herpesvirus research
- RNA Interference and Gene Delivery
- Polyomavirus and related diseases
- Respiratory viral infections research
- Neurobiology and Insect Physiology Research
- Influenza Virus Research Studies
- Advanced Fluorescence Microscopy Techniques
- Virus-based gene therapy research
- Fetal and Pediatric Neurological Disorders
- Cerebrospinal fluid and hydrocephalus
- CRISPR and Genetic Engineering
- HIV/AIDS Research and Interventions
- Biochemical and Molecular Research
National University of Singapore
2025
UConn Health
2023-2025
Inner Mongolia Normal University
2023-2024
Henan Agricultural University
2024
Shandong University
2024
Shandong Provincial Hospital
2024
Shandong First Medical University
2024
Qilu Hospital of Shandong University
2024
Bureau of Geology and Mineral Exploration and Development of Guizhou Province
2024
University of Nevada, Reno
2019-2023
Various morphologies of magnesium carbonate hydrates have been synthesized by carefully adjusting the reaction temperature and pH value initial solution in precipitation process. At lower temperatures (from room to 328 K) values (variation with temperature), are prone display needlelike morphology, axis diameter particles decreases increase value. With further (333−368 value, sheetlike crystallites become preferred at higher values, these tend assemble into layerlike structures diverse...
Although mesenchymal stem cell (MSC) transplantation has been shown to promote cardiac repair in acute myocardial injury vivo, its overall restorative capacity appears be restricted mainly because of poor viability and low engraftment the ischemic myocardium. Specific chemokines are upregulated infarcted However expression levels corresponding chemokine receptors (eg, CCR1, CXCR2) MSCs very low. We hypothesized that this discordance may account for MSC survival.To determine whether...
A sensitive assay for melamine in complex matrices is built using cysteamine-modified gold nanoparticles (AuNPs) and an effective sample pretreatment protocol. Citrate-stabilized AuNPs were modified by cysteamine order to weaken the electrostatic repulsion force between nanoparticles. Detection sensitivity gained through this modification increased about 100 fold compared with result unmodified AuNPs. Direct colorimetric visualizations of milk products, eggs feeds was successfully...
Atherosclerosis is a leading cause of death globally. Targeted imaging and therapeutics are desirable for the detection treatment disease. In this study, we developed trifunctional Simian virus 40 (SV40)-based nanoparticles in vivo targeting atherosclerotic plaques. These novel SV40-based encapsulate near-infrared quantum dots bear element drug component. Using nanoparticles, were able to noninvasively fluorescently image plaques live intact ApoE(−/−) mice. Near-infrared encapsulated SV40...
Abstract Cross‐protective and non‐invasively administered vaccines are attractive highly desired for the control of influenza. Self‐assembling nanotechnology provides an opportunity development with superior performance. In this study, intranasal nanovaccine is developed targeting conserved ectodomain influenza matrix protein 2(M2e). 3‐sequential repeats M2e (3M2e) presented on self‐assembling recombinant human heavy chain ferritin (rHF) cage to form 3M2e‐rHF nanoparticle. Intranasal...
Abstract Protein homeostasis is critical for health and lifespan of animals. However, the mechanisms controlling protein feeding remain poorly understood. Here we report that in Drosophila , intake-induced inhibition (PIFI) specific to protein-containing food, this effect mediated by a fat body (FB) peptide named female-specific independent transformer (FIT). Upon consumption FIT expression greatly elevated. Secreted fly haemolymph conveys metabolic message brain, thereby promoting release...
Significance Influenza A virus (IAV) is one of the most important human pathogens, and it crucial to understand its life cycle develop antiviral strategies. However, IAV uncoating, an essential step in viral infection, has remained incomprehensible. Here, via construction infectious virions encapsulating quantum dots, we tracked uncoating ribonucleoprotein complex (vRNP) dynamics single virions. Our results reveal that after fusion vRNP segments are released separately into cytosol,...
Detection of Bacillus anthracis in the field, whether as a natural infection or biothreat remains challenging. Here we have developed new lateral-flow immunochromatographic assay (LFIA) for B. spore detection based on fact that conjugates spores and super-paramagnetic particles labeled with antibodies will block pores chromatographic strips form retention lines strips, instead conventionally reported test control classic LFIA. As result, this LFIA can simultaneously realize optical, magnetic...
Poor understanding of the basic biology Mycobacterium tuberculosis (MTB), etiological agent tuberculosis, hampers development much-needed drugs, vaccines, and diagnostic tests. Better experimental tools are needed to expedite investigations this pathogen at systems level. Here, we present a functional MTB proteome microarray covering most an ORFome library. We demonstrate broad applicability by investigating global protein-protein interactions, small-molecule-protein binding, serum biomarker...
Macrophages are one of the major targets human immunodeficiency virus (HIV-1), but viral entry pathway remains poorly understood in these cells. Noninvasive labeling and single-virus tracking effective tools for studying entry. Here, we constructed a quantum dot (QD)-encapsulated infectious HIV-1 particle to track at single-particle level live primary macrophages. QDs were encapsulated virions by incorporating accessory protein Vpr-conjugated during assembly. With particles encapsulating...
The nicotinamidase/pyrazinamidase (PncA) of Mycobacterium tuberculosis is involved in the activation important front-line antituberculosis drug pyrazinamide by converting it into active form, pyrazinoic acid. Mutations pncA gene cause resistance M. tuberculosis. properties PncA were characterized this study. enzyme was found to be a 20.89 kDa monomeric protein. optimal pH and temperature enzymatic activity 7.0 40 degrees C, respectively. Inductively coupled plasma-optical emission...
Aiming to build a supersensitive and easily operable immunoassay, bifunctional protein nanowires were generated by seeding-induced self-assembling of the yeast amyloid Sup35p that genetically fused with G an enzyme (methyl-parathion hydrolase, MPH), respectively. The possessed high ratio molecules G, allowing dramatic increase enzymatic signal when was bound antibody target. As result, 100-fold enhancement sensitivity obtained applied in detection Yersinia pestis F1 antigen.
The fluorescence labeling of viruses is a useful technology for virus detection and imaging. By combining the excellent properties quantum dots (QDs) with high affinity specificity aptamers, we constructed QD–aptamer probe. aptamer A22, against hemagglutinin influenza A virus, was linked to QDs, producing QD–A22 Fluorescence imaging transmission electron microscopy showed that probe could specifically recognize label particles. This QD technique provides new strategy particles
Imaging of protein–protein and RNA–protein interactions in vivo, especially live animals, is still challenging. Here we developed far-red mNeptune-based bimolecular fluorescence complementation (BiFC) trimolecular (TriFC) systems with excitation emission above 600 nm the 'tissue optical window' for imaging cells mice. The mNeptune BiFC was first built by selecting appropriate split fragments, then mNeptune-TriFC system based on mNeptune-BiFC system. newly constructed TriFC were verified as...
Abstract Single genomic loci are often related to specific cellular functions, genetic diseases, or pathogenic infections. Visualization of single in live human cells is currently great interest, yet it remains challenging. Here, we describe a strategy for cell imaging by combining transcription activator-like effectors (TALEs) with quantum dot labelling technique. We design and select pair TALEs that specifically target HIV-1 proviral DNA sequences, use bioorthogonal ligation reactions...
In Populus, the transcripts of fasciclin-like arabinogalactan proteins (FLAs) are accumulated in tension wood (TW) xylem, however their biological functions TW formation largely unknown. this work, we demonstrated that PtFLA6, one poplar TW-associated PtFLAs, was abundantly expressed TW, and mainly localized differentiating G-fibers. The bended stems PtFLA6 antisense transgenic showed decreased including reduced like proteins, leading to inhibited differentiation formation. We also gibberellin A3 (GA