A5057640178- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
- Bacillus and Francisella bacterial research
- Extracellular vesicles in disease
- Biosensors and Analytical Detection
- CRISPR and Genetic Engineering
- Geological and Geochemical Analysis
- Biochemical and Structural Characterization
- Electrochemical sensors and biosensors
- bioluminescence and chemiluminescence research
- RNA and protein synthesis mechanisms
- vaccines and immunoinformatics approaches
- RNA Interference and Gene Delivery
- Tuberculosis Research and Epidemiology
- Advanced Fluorescence Microscopy Techniques
- SARS-CoV-2 and COVID-19 Research
- MicroRNA in disease regulation
- COVID-19 Clinical Research Studies
- interferon and immune responses
- Monoclonal and Polyclonal Antibodies Research
- Click Chemistry and Applications
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Immune cells in cancer
- Antibiotic Resistance in Bacteria
Institute of Biophysics
2015-2024
Chinese Academy of Sciences
2015-2024
Peking University
2022-2023
Shandong University of Finance and Economics
2021-2022
University of Chinese Academy of Sciences
2021
Center for Excellence in Education
2018-2019
Wuhan Institute of Virology
2007-2014
Huazhong Agricultural University
2008-2009
We report a rolling circle amplification and nicking endonuclease-assisted dual signal fluorescent biosensor for exosome detection.
Abstract Bimodal synergistic therapy produces superadditive effect for enhanced therapeutic efficacy. However, how to efficiently and simultaneously deliver several kinds of agents is still challenging. A cancer cell membrane‐derived nanocarrier (mCas9‐sGNRs) proposed photothermal/gene (PTT/GT) by efficient delivery clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR‐associated protein 9 (Cas9) gold nanorods (GNRs). In this approach, Cas9 proteins can be loaded inside...
Detection of Bacillus anthracis in the field, whether as a natural infection or biothreat remains challenging. Here we have developed new lateral-flow immunochromatographic assay (LFIA) for B. spore detection based on fact that conjugates spores and super-paramagnetic particles labeled with antibodies will block pores chromatographic strips form retention lines strips, instead conventionally reported test control classic LFIA. As result, this LFIA can simultaneously realize optical, magnetic...
Poor understanding of the basic biology Mycobacterium tuberculosis (MTB), etiological agent tuberculosis, hampers development much-needed drugs, vaccines, and diagnostic tests. Better experimental tools are needed to expedite investigations this pathogen at systems level. Here, we present a functional MTB proteome microarray covering most an ORFome library. We demonstrate broad applicability by investigating global protein-protein interactions, small-molecule-protein binding, serum biomarker...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes disease 2019, constitutes an emerging human pathogen of zoonotic origin. A critical role in protecting the host against invading pathogens is carried out by interferon-stimulated genes (ISGs), primary effectors type I interferon (IFN) response. All coronaviruses studied thus far have to first overcome inhibitory effects IFN/ISG system before establishing efficient viral replication. However, whether SARS-CoV-2 evades...
Abstract Antimicrobial peptides (AMPs) are a promising solution for treating antibiotic‐resistant pathogens. However, efficient generation of diverse AMPs without prior knowledge peptide structures or sequence alignments remains challenge. Here, ProT‐Diff is introduced, modularized deep generative approach that combines pretrained protein language model with diffusion the de novo sequences. generates thousands lengths and within few hours. After silico physicochemical screening, 45 selected...
Abstract Exosomes are a novel and promising drug delivery platform because of their endogenous origin, stability, biocompatibility, other unique features. As the efficient loading long RNA to target cells for therapeutic purposes remains challenging, new exosome‐based system is proposed using controllable enrichment releasing protocol. The employs aptamer–protein interactions reversible light‐inducible protein–protein interaction modules by remolding exosome producer cells. Endogenous...
Biofilms can cause many bacterial diseases, such as dental disease. An in vitro detection of biofilms may help to screen antibiofilm drugs. impedance measurement based on an Au electrode has been successfully used for real-time monitoring animal and human cell growth. However, microbial growth the produced a poor signal because small size cells. We have recently demonstrated that graphene derivatives be carbon through facile electrochemical activation, thus forming reduced oxide-carbon...
Abstract Antimicrobial Peptides (AMPs) represent a promising class of antimicrobial agents crucial for combating antibiotic-resistant pathogens. Despite the emergence deep learning approaches AMP discovery, there remains gap in efficiently generating novel AMPs across various amino acid lengths without prior knowledge peptide structures or sequence alignments. Here we introduce ProT-Diff, modularized and efficient generative approach that ingeniously combines pre-trained protein language...
As a potential antimicrobial, the bacteriophage lysin PlyG has been reported to specifically recognize Bacillus anthracis vegetative cells only and kill B. its germinating spores. However, how interacts with spores remains unclear. Herein, 60-amino-acid domain in (residues 106 165), located mainly previously identified catalytic domain, was found able but not cells. The exosporium of be most probable binding target this domain. This is first time that for spore-forming bacteria have separate...
The self-assembly of nanoparticles into larger superstructures is a powerful strategy to develop novel functional nanomaterials, as these display collective properties that are different those displayed by individual or bulk samples. However, there increasing bottlenecks in terms size control and multifunctionalization nanoparticle assemblies. In this study, we developed for construction multifunctional assemblies tunable size, through rational regulation the number self-assembling...
Acute myeloid leukemia (AML) is a heterogeneous disease, and there are critical interests in detecting multiple biomarkers as single biomarker detection cannot reflect the exact phase of disease. Exosomes derived from different types AML cells contain respective combinations cluster differentiation (CD) markers that may be used to guide molecular typing clinic. Here, aiming build more precise AML, we demonstrate multiplex immuno-PCR (mI-PCR) assay for simultaneous surface CDs on exosomes via...
ConspectusBacillus anthracis, present in two forms of vegetative cells and spores, is a pathogen that infects humans through contact with infected animals or contaminated animal products also maliciously used terrorist acts. Therefore, rapid sensitive test for B. anthracis necessary but challenging. The challenge comes from the following aspects: an accurate distinction other Bacillus species due to their high genomic similarity horizontal gene transfer between members; direct detection...
Protein phosphorylation on serine, threonine, and tyrosine (Ser/Thr/Tyr) is well established as a key regulatory posttranslational modification used in signal transduction to control cell growth, proliferation, stress responses. However, little known about its extent function diatoms. Phaeodactylum tricornutum unicellular marine diatom that has been model organism for research molecular biology. Although more than 1000 protein kinases phosphatases with specificity Ser/Thr/Tyr residues have...
Bacillus anthracis, the causative agent of anthrax disease, could be used as a biothreat reagent. It is vital to develop rapid, convenient method detect B. anthracis. In current study, three high affinity and specificity monoclonal antibodies (mAbs, designated 8G3, 10C6 12F6) have been obtained using fully washed anthracis spores an immunogen. These mAbs, confirmed direct against EA1 protein, can recognize surface intact vegetative cells with species-specificity. has well known major S-layer...
This study demonstrates the first use of surface plasmon resonance (SPR) technology for rapid, sensitive and label-free detection whole B. anthracis spores. The approach involves an SPR biosensor (Biacore® 3000), a monoclonal antibody which was raised against spore (mAb 8G3). By means subtractive inhibition assays, spores with concentrations as low 104 colony-forming units (CFU) ml−1 can be detected within 40 min, other related Bacillus spores, even in high concentrations, differentiated from