A5109937672- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Biochemical and Molecular Research
- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Cancer therapeutics and mechanisms
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
- Pneumocystis jirovecii pneumonia detection and treatment
- Advanced Biosensing Techniques and Applications
- Bacterial Genetics and Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- Biotin and Related Studies
- DNA and Nucleic Acid Chemistry
- DNA Repair Mechanisms
- Biosensors and Analytical Detection
- Folate and B Vitamins Research
- Gene expression and cancer classification
- Antimicrobial Peptides and Activities
- Adenosine and Purinergic Signaling
- Cancer Research and Treatments
- Sirtuins and Resveratrol in Medicine
- vaccines and immunoinformatics approaches
Chinese Academy of Sciences
2014-2024
Wuhan Institute of Virology
2014-2024
Guangzhou Medical University
2023
Guangzhou Women and Children Medical Center
2023
Key Laboratory of Guangdong Province
2017-2022
Sichuan Agricultural University
2012
Albert Einstein College of Medicine
2010-2011
University at Albany, State University of New York
2011
Howard Hughes Medical Institute
2011
Kaplan Medical Center
2011
The lysine acetylation of proteins is a reversible post-translational modification that plays critical regulatory role in both eukaryotes and prokaryotes. Mycobacterium tuberculosis facultative intracellular pathogen the causative agent tuberculosis. Increasing evidence shows may play an important pathogenesis M. However, only few acetylated are known, presenting major obstacle to understanding functional roles this pathogen. We performed global acetylome analysis H37Ra by combining...
A sensitive assay for melamine in complex matrices is built using cysteamine-modified gold nanoparticles (AuNPs) and an effective sample pretreatment protocol. Citrate-stabilized AuNPs were modified by cysteamine order to weaken the electrostatic repulsion force between nanoparticles. Detection sensitivity gained through this modification increased about 100 fold compared with result unmodified AuNPs. Direct colorimetric visualizations of milk products, eggs feeds was successfully...
Mycobacterium tuberculosis (Mtb), the causative agent of human tuberculosis, remains one most prevalent pathogens and a major cause mortality worldwide. Metabolic network is central mediator defining feature pathogenicity Mtb. Increasing evidence suggests that lysine succinylation dynamically regulates enzymes in carbon metabolism both bacteria cells; however, its extent function Mtb remain unexplored. Here, we performed global succinylome analysis virulent strain H37Rv by using high...
Poor understanding of the basic biology Mycobacterium tuberculosis (MTB), etiological agent tuberculosis, hampers development much-needed drugs, vaccines, and diagnostic tests. Better experimental tools are needed to expedite investigations this pathogen at systems level. Here, we present a functional MTB proteome microarray covering most an ORFome library. We demonstrate broad applicability by investigating global protein-protein interactions, small-molecule-protein binding, serum biomarker...
During the course of infection, Mycobacterium tuberculosis (Mtb) is exposed to diverse redox stresses that trigger metabolic and physiological changes. How these stressors are sensed relayed Mtb transcriptional apparatus remains unclear. Here, we provide evidence WhiB6 differentially regulates ESX-1 DosR regulons through its Fe-S cluster. When challenged with NO, continually activates expression but initial activation followed by gradual inhibition. Comparative transcriptomic analysis holo-...
The nicotinamidase/pyrazinamidase (PncA) of Mycobacterium tuberculosis is involved in the activation important front-line antituberculosis drug pyrazinamide by converting it into active form, pyrazinoic acid. Mutations pncA gene cause resistance M. tuberculosis. properties PncA were characterized this study. enzyme was found to be a 20.89 kDa monomeric protein. optimal pH and temperature enzymatic activity 7.0 40 degrees C, respectively. Inductively coupled plasma-optical emission...
The silent information regulator (Sir2) family proteins are NAD+-dependent deacetylases. Although a few substrates have been identified, functions of the bacteria Sir2-like protein (CobB) still remain unclear. Here role CobB on Escherichia coli chemotaxis was investigated. We used Western blotting and mass spectrometry to show that response CheY is substrate CobB. Surface plasmon resonance (SPR) indicated acetylation affects interaction between flagellar switch FliM. presence intact flagella...
The fluorescence labeling of viruses is a useful technology for virus detection and imaging. By combining the excellent properties quantum dots (QDs) with high affinity specificity aptamers, we constructed QD–aptamer probe. aptamer A22, against hemagglutinin influenza A virus, was linked to QDs, producing QD–A22 Fluorescence imaging transmission electron microscopy showed that probe could specifically recognize label particles. This QD technique provides new strategy particles
Imaging of protein–protein and RNA–protein interactions in vivo, especially live animals, is still challenging. Here we developed far-red mNeptune-based bimolecular fluorescence complementation (BiFC) trimolecular (TriFC) systems with excitation emission above 600 nm the 'tissue optical window' for imaging cells mice. The mNeptune BiFC was first built by selecting appropriate split fragments, then mNeptune-TriFC system based on mNeptune-BiFC system. newly constructed TriFC were verified as...
DNA gyrase is an indispensible marvelous molecular machine in manipulating the topology for prokaryotes. In ‘two-gate’ mechanism of topoisomerase, T-segment navigation from N- to DNA-gate a critical step, but structural basis supporting this scheme unclear. The crystal structure B′ subfragment Mycobacterium tuberculosis reveals intrinsic homodimer. two subunits, each consisting Tail and Toprim domain, are tightly packed one another form ‘crab-like’ organization never observed previously...
Abstract Viral capsid–nanoparticle hybrid structures offer new opportunities for nanobiotechnology. We previously generated virus‐based nanoparticles (VNPs) of simian virus 40 (SV40) containing quantum dots (QDs) cellular imaging. However, as an interesting issue nano‐bio interfaces, the mechanism nanoparticle (NP) encapsulation by viral coat proteins remains unclear. Here, four kinds QDs with same core/shell but different surface coatings are tested encapsulation. All can be encapsulated...
Wire to wire: The hybrid Sup351-61-E2GFP-MPH, which consists of the pH-sensitive green fluorescent protein E2GFP, enzyme methyl parathion hydrolase (MPH), and yeast prion Sup351-61 was obtained by gene fusion. A self-assembled nanowire formed through aggregation subunit (see picture). wire acts as a biosensor that can detect with sensitivity 10 000 times greater than free E2GFP-MPH. Detailed facts importance specialist readers are published "Supporting Information". Such documents...
As a potential antimicrobial, the bacteriophage lysin PlyG has been reported to specifically recognize Bacillus anthracis vegetative cells only and kill B. its germinating spores. However, how interacts with spores remains unclear. Herein, 60-amino-acid domain in (residues 106 165), located mainly previously identified catalytic domain, was found able but not cells. The exosporium of be most probable binding target this domain. This is first time that for spore-forming bacteria have separate...
The self-assembly of nanoparticles into larger superstructures is a powerful strategy to develop novel functional nanomaterials, as these display collective properties that are different those displayed by individual or bulk samples. However, there increasing bottlenecks in terms size control and multifunctionalization nanoparticle assemblies. In this study, we developed for construction multifunctional assemblies tunable size, through rational regulation the number self-assembling...
Tuberculosis is still on the top of infectious diseases list both mobility and mortality, especially due to drug-resistance Mycobacterium tuberculosis (M.tb). Ethionamide (ETH) one effective second line anti-TB drugs, a synthetic compound similar isoniazid (INH) structurally, with existing severe problem ETH resistance. prodrug, which activated by Etha inside M.tb, etha transcriptionally repressed Ethr. We found that c-di-GMP could bind Ethr, enhanced binding Ethr promoter etha, then...
NADH pyrophosphatase (NudC) catalyses the hydrolysis of NAD(H) to AMP and NMN(H) [nicotinamide mononucleotide (reduced form)]. NudC multiple sequence alignment reveals that homologues from most Mycobacterium tuberculosis isolates, but not other mycobacterial species, have a polymorphism at highly conserved residue 237. To elucidate functional significance this polymorphism, comparative analyses were performed using representative isoforms M. H37Rv (NudC(Rv)) bovis BCG (NudC(BCG))....
DNA gyrase plays a vital role in resolving topological problems and is the target of antibiotics such as fluoroquinolones. Mycobacterium fluoroquinolone resistance protein A (MfpA) from smegmatis newly identified inhibitor that believed to confer intrinsic However, MfpA does not prevent drug-induced inhibition vitro , implying involvement other yet unknown factors. Here, we have new factor, named B (MfpB), which involved protection against drugs both vivo . Genetic results suggest MfpB...
Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis, leading cause death among all infectious diseases. There are 11 eukaryotic-like serine/threonine protein kinases (STPKs) in Mtb, which thought to play pivotal roles cell growth, signal transduction and pathogenesis. However, their underlying mechanisms action remain largely uncharacterized. In this study, using a Mtb proteome microarray, we have globally identified binding proteins for STPKs, constructed first STPK...
Although MgrB is established to be a feedback inhibitor of the PhoP/Q system in Escherichia coli, biological functions remain largely unknown. To explore new MgrB, comparative transcriptome analysis was performed (E. coli K-12 W3110 ΔmgrB vs E. W3110). The results showed that many genes involved acid stress are upregulated, suggesting related sensitivity coli. survival rates under mutant and wild-type deletion mgrB resulted resistance. According previous research, we deleted phoP, phoQ iraM...
Acetyl-CoA (AcCoA) synthetase (Acs) catalyzes the conversion of acetate into AcCoA, which is involved in many catabolic and anabolic pathways.Although this enzyme has been studied for years organisms, properties Mycobacterium tuberculosis Acs regulation its activity remain unknown.Here, putative acs gene M. H37Rv (Mt-Acs) was expressed as a fusion protein with 63His-tag on C-terminus Escherichia coli.The recombinant Mt-Acs successfully purified then enzymatic characteristics were...
Live cell imaging of mRNA-protein interactions makes it possible to study posttranscriptional processes cellular and viral gene expression under physiological conditions. In this study, red color mCherry-based trimolecular fluorescence complementation (TriFC) systems were constructed as new tools for visualizing mRNA–protein interaction in living cells using split mCherry fragments HIV REV-RRE TAT-TAR peptide-RNA pairs. The TriFC successfully used image RNA–protein such that between...