Lycoris longituba is one of the species belonging to Amaryllidaceae family. Despite its limited distribution, endemic central eastern China, this displays an exceptionally wide diversity flower colors from purple, red, orange, yellow, in nature. We study natural variation floral color L. by testing components water-soluble vacuolar pigments – anthocyanins petals using high-performance liquid chromatography coupled with photodiode array detection and electrospray ionization mass spectrometry....

Aberrant production of H2O2 is involved in cancer. The levels are significantly higher tumor cells than normal cells. It important to develop fluorescent probes image basal selectively So far, a cancer cell-targeting probe has not been reported. Thus, we developed probe, BBHP, which contains benzil as H2O2-recognition site and biotin target binding motif for the selective sufficient detection BBHP enables fluorescence turn-on response H2O2. with receptors can greatly accelerate As result,...

Abstract Single genomic loci are often related to specific cellular functions, genetic diseases, or pathogenic infections. Visualization of single in live human cells is currently great interest, yet it remains challenging. Here, we describe a strategy for cell imaging by combining transcription activator-like effectors (TALEs) with quantum dot labelling technique. We design and select pair TALEs that specifically target HIV-1 proviral DNA sequences, use bioorthogonal ligation reactions...

To study the problem of male sterility cattle-yak and improve yak crossbreeding, this obtained testicular Sertoli cells compared differences in transcriptome levels between two bovine species. The tissues 3 healthy cattle-yaks F1 generation yaks were collected at age 24 months. isolated after enzymatic digestion, differential adhesion starvation treatment. DATA-4 SOX9 immunofluorescence staining used to identify cell type. subjected sequencing, GO analysis, KEGG analysis differentially...

Plant cytochrome P450s are involved in a wide range of biosynthetic reactions that generate various biomolecules, including variety defensive compounds. Perennial ryegrass (Lolium perenne) and tall fescue (Festuca arundinacea) two major species turf forage grasses usually experience low temperature below -10°C high over 38°C around the world. In this study, we re-analyzed transcriptome perennial treated with heat cold stress. Thus, can evaluate P450 composition these confirm whether genes...

The logistic or S-shaped curve of growth is one the few universal laws in biology. It certain that there exist specific genes affecting curves, but, due to a lack statistical models, it unclear how these cause phenotypic differentiation and developmental trajectories. In this paper we present model for detecting major responsible This incorporated with pervasive curves under maximum likelihood framework and, thus, expected improve over previous models both parameter estimation inference....

HIV latency is one of the major problems in HIV/AIDS cure. Imaging single-copy integrated proviral DNA host cell has both virology and clinical significance but remains technical challenge. Here, we developed a dual-color labeled CRISPR system to image HIV-1 latently infected cells. The pair CRISPRs was fluorescently with two different color QDs using alternative bioorthogonal ligation reactions. Integrated HIV-sequences are successfully mapped based on colocalized signals living Compared...

After the COVID-19 outbreak, many Chinese high school students have increased their dependence on electronic devices for studying and life, which may affect incidence of neck shoulder pain (NSP) in adolescents.To evaluate prevalence NSP its associated risk factors during COVID-19, a survey was conducted among 5,046 Shanghai, Qinghai, Henan Macao second semester summer vacation 2019-2020 academic year. The questionnaire included questions regarding demographic characteristics, lifestyle such...

Faced with rising threats of terrorism, environmental and health risks, achieving sensitive selective detection peroxide-based explosives (PEs) has become a global focus. In this study, turn-on fluorescent probe (BOD) based on benzil (H2O2-recognition element) 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) derivative (fluorophore) was developed to sensitively specifically detect hydrogen peroxide (H2O2). The synthesized BOD had very weak fluorescence due intramolecular donor-excited...

Acute myeloid leukemia (AML) is a fatal hematologic disease. Diagnosis and proper treatment are important for prognosis. High myeloperoxidase (MPO) expression AML cells characterized with high levels of hypochlorite (ClO–). In this study, we report ClO–-activated theranostic agent, FNC, therapy. FNC responds to ClO– specifically in MPO cells, resulting bright fluorescence chlorambucil release. can be used quickly distinguish from other including low activated inflammatory cells. exhibits...

The concentration of total suspended matter (TSM) is an important parameter for evaluating lake water quality. We determined in situ hyperspectral data and TSM Changdang Lake, China, to establish a inversion model. model was applied using 60 Sentinel-2 images acquired from 2016 2021 determine the temporal spatial distribution concentration. Remote sensing were also utilized monitor effect ecological dredging Lake. following results obtained: (1) Compared with four existing models,...

基于卫星遥感的水体提取算法对面积较大的水体效果较好,应用于细小水体时受混合像元、异物同谱等因素影响,容易出现误判。Sentinel-2卫星多光谱遥感数据空间分辨率为10 m、20 m、60 m,双星时间分辨率5 d,时间和空间分辨率较高,因此本文采用了Sentinel-2绿光波段(560 nm)、红边波段(705 nm)、近红外波段(842 nm、865 nm)和短波红外波段(2190 nm)的遥感反射率,提出了一种植被红边水体指数算法RWI(Vegetation Red Edge based Water Index)。对比分析了植被、阴影、建筑物、混合像元、裸土、水体6种地物的归一化遥感反射率,从机理上解释了为什么RWI比其他水体指数具有更好的提取细小水体的效果。本文对比了常用的几种水体提取算法,包括改进的归一化差异水体指数MNDWI(Modified Normalized Difference Index)、多波段水体指数MBWI(Multi-Band Index)、自动水提取指数AWEI(Automated Extraction...

高分辨率卫星的幅宽一般很小,受云雨和轨道回访周期影响,单颗卫星的短时段内覆盖能力有限,因此,单一高分辨率卫星常常无法满足一定时段内的黑臭水体监测需求,需要多源卫星协同监测黑臭水体。为了分析多源高分辨率影像对黑臭水体遥感监测的适用性,本文基于地物光谱仪实测的水体遥感反射率数据,以GeoEye-1、WorldView-2、北京二号(DMC3)、高景一号SV1(SuperView-1)以及GF-PMS系列(GF-1/1B/1C/1D、GF-2、GF-6)传感器波段进行等效计算,结果表明:(1)采用反射率比值模型——BOI(Black and Odorous water...

Background. Landoltia punctata can be used as renewable and sustainable biofuel feedstock because it quickly accumulate high starch levels. ADP-glucose pyrophosphorylase (AGPase) catalyzes the first committed step during biosynthesis in higher plants. The heterotetrameric structure of plant AGPases comprises pairs large subunits (LSs) small (SSs). Although several studies have reported on accumulation capacity duckweed, no study has explored underlying molecular mechanisms their linkage with...

Airborne optical images (AOI) are often with complex sunglint reflections, which brings a certain influence to watercolor retrieval. This includes the reflection water surface statistical distribution characteristics caused by imaging viewing angles differences, high spatial resolution discrete sharing similar angles, and Fresnel differences skylight difference during flight of unmanned aerial vehicles. Aiming at multiscale in AOI, based on multi-path radiation transmission, interference...