A5100743120- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Urologic and reproductive health conditions
- Sexual Differentiation and Disorders
- Ovarian function and disorders
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Testicular diseases and treatments
- Inflammatory Bowel Disease
- Sexual function and dysfunction studies
- DNA Repair Mechanisms
- Male Reproductive Health Studies
- Urological Disorders and Treatments
- Genital Health and Disease
- Gut microbiota and health
- Hormonal and reproductive studies
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Hernia repair and management
- Pelvic floor disorders treatments
- Genomics and Chromatin Dynamics
- Colorectal Cancer Surgical Treatments
- Microscopic Colitis
- Renal and related cancers
- Genomic variations and chromosomal abnormalities
- Diet and metabolism studies
Shanghai Jiao Tong University
2014-2025
Shanghai First People's Hospital
2017-2025
Shanghai Ninth People's Hospital
2019-2025
Beijing Hospital of Traditional Chinese Medicine
2023-2025
Capital Medical University
2023-2025
Jiangxi Normal University
2024
Fuzhou University
2024
Guangdong Medical College
2023
First Affiliated Hospital of Soochow University
2017
Soochow University
2013-2017
MicroRNAs (miRNAs) play important roles in mammalian spermatogenesis, which is highly dependent on Sertoli cells. However, the functions and mechanisms of miRNAs regulating human cells remain largely unknown. Here, we report that hsa-miR-202-3p mediates proliferation, apoptosis, synthesis function miR-202-3p was upregulated cell-only syndrome (SCOS) patients compared with obstructive azoospermia (OA) normal spermatogenesis. Overexpression induced cell apoptosis inhibited proliferation...
Abstract Nonobstructive azoospermia (NOA), one of the most severe types male infertility, etiology often remains unclear in cases. Therefore, this study aimed to detect four biallelic detrimental variants (0.5%) minichromosome maintenance domain containing 2 ( MCMDC2 ) genes 768 NOA patients by whole-exome sequencing (WES). Hematoxylin and eosin (H&E) demonstrated that deleterious caused meiotic arrest three (c.1360G>T, c.1956G>T, c.685C>T) hypospermatogenesis patient...
Objectives: To identify transfusion thresholds and risk factors for acute kidney injury (AKI) in gastrointestinal oncology surgery, enhancing early intervention improving postoperative outcomes. Methods: From 2018 to 2022, 765 patients with gastric or colorectal cancer who underwent major surgery were retrospectively enrolled. The primary outcome was AKI development within 7 days postoperatively. Clinicopathological characteristics short-term outcomes recorded compared. Results: Of all...
At present, non-invasive methods are not comprehensive enough to enable urologists predict sperm retrieval results accurately in patients with non-obstructive azoospermia (NOA). Our aim was improve the prediction accuracy of by using leptin and artificial neural networks (ANNs).Data from May 2004 July 2010 for 280 NOA were reviewed assigned into training testing set ANNs. All underwent standard diagnostic infertility evaluation testicular extraction (TESE). Twelve factors recorded as input...
We have previously shown that the transcript levels of Vegfc and its receptor Vegfr3 were high in spermatogonia extremely low spermatocytes spermatids. However, it remains unknown about functions mechanisms VEGFC/VEGFR3 signaling regulating fate determinations spermatogonia. To this end, here we explored role pathways by using a cell line derived from immortalized mouse retaining markers mitotic germ cells, namely GC-1 cells. VEGFR3 was expressed primary VEGFC stimulated proliferation DNA...
Non-obstructive azoospermia (NOA) is one of the most severe type in male infertility, and genetic causes NOA with meiotic arrest remain elusive.Four Chinese families participated study. We performed whole-exome sequencing (WES) for four NOA-affected patients pedigrees. The candidate causative gene was further verified by Sanger sequencing. Hematoxylin eosin staining (H&E) immunohistochemistry (IHC) were carried out to evaluate stage spermatogenesis arrested NOA.We identified two novel...
Background The genetic causes of human idiopathic non-obstructive azoospermia (NOA) with meiotic arrest remain unclear. Methods Two Chinese families infertility participated in the study. In family 1, two brothers were affected by NOA. 2, proband was diagnosed NOA, and his elder sister suffered from infertility. Whole-exome sequencing (WES) conducted patients 2 362 additional sporadic Sanger used to verify WES results. Periodic acid–Schiff (PAS), immunohistochemistry (IHC) chromosomal spread...
Abstract Objectives RNA‐binding proteins (RBPs) play essential post‐transcriptional roles in regulating spermatogonial stem cells (SSCs) maintenance and differentiation. We identified a conserved SSCs‐enriched RBP ELAVL2 from our single‐cell sequencing data, but its function mechanism SSCs were unclear. Materials methods Expressions of during human mouse testis development validated. Stable C18‐4 TCam‐2 cell lines with overexpression knockdown established, which applied to proliferation...
Stepwise mini-incision microdissection testicular sperm extraction (mTESE) is a procedure that attempts to minimize damage. However, the approach may vary in patients with different etiologies. Here, we performed retrospective analysis of 665 men nonobstructive azoospermia (NOA) who underwent stepwise mTESE (Group 1) and 365 standard 2). The results showed operation time (mean ± deviation) for successful retrieval Group 1 (64.0 26.6 min) was significantly shorter than 2 (80.2 31.3 min), P...
Effects of acute tadalafil on sperm motility and acrosome reaction were investigated, both in vitro vivo. Twenty asthenozoospermic 20 normozoospermic patients as control randomly enrolled. For part, 0.5 ml solutions with different concentrations added (0.2, 0.1, 0.05 0.025 μg ml−1, respectively) into semen samples. In groups, samples treated 0.2 ml−1 had significant increase after 2 h incubation. vivo oral administration (20 mg) or sildenafil (100 was given. computer-assisted analysis...
Excessive apoptotic spermatozoon death is associated with male infertility. Leptin regulates apoptosis in several cell types. We prospectively investigated if seminal plasma leptin mediates 74 varicocele (VC) patients and 70 leucocytospermia patients. Spermatozoa from 40 normospermic men were used as controls. Routine semen analysis, rate, leptin, reactive oxygen species (ROS) tumour necrosis factor-alpha (TNF-α) levels measured. In VC patients, rates significantly higher compared the group,...
KASH5 is an essential component of the LINC (linker nucleoskeleton and cytoskeleton) complex that regulates chromosome movements nuclear envelope (NE) remodeling in mouse spermatocytes during meiosis prophase I, but its expression function human cells, as well association with male infertility are largely unknown. In this study, a novel heterozygous copy number variation (CNV) (seq [GRCh37] del(19) (19q13.33) chr19: g.49894043-49903011del) loss variant (NM_144688: c.979_980del: p.R327Sfs*21)...
Abstract The genetic causes of idiopathic premature ovarian insufficiency (POI) and nonobstructive azoospermia (NOA) remain unclear. We performed whole‐exome sequencing (WES) in members a consanguineous family with two POI NOA patients to screen for potential pathogenic variants familial NOA. And homozygous variant SPATA22 (c.400C>T:p.R134X) was identified. Histological analysis spermatocyte spreading assay demonstrated that the spermatogenesis arrested at zygotene‐like stage proband...
Aging has a significant negative impact on human testicular function; steroidogenesis is gradually impaired, and testosterone replacement therapy still many risks. Low-intensity pulsed ultrasound (LIPUS) been used as novel non-invasive treatment for male erectile dysfunction other fields, shown to increase levels in animal models. Testosterone synthesized secreted by Leydig cells (LCs), the serum level decreases after aging due LCs senescence. However, effect of LIPUS senescent not reported....
Abstract: Transverse testicular ectopia (TTE) associated with persistent Mullerian duct syndrome (PMDS) is a rare form of male pseudohermaphroditism usually unexpectedly found at surgery for cryptorchidism or inguinal hernia in children. Its etiology and prevalence are unclear, although defects the gene that encodes anti-Mullerian hormone (AMH) AMH receptor has been generally considered as major cause. Adult cases TTE PMDS even more peculiar, adult patients present complex medical history,...
Non-obstructive azoospermia (NOA) is the most severe disease in male infertility, but genetic causes for majority of NOA remain unknown. FANCM a member Fanconi Anemia (FA) core complex, whose defects are associated with cell hypersensitivity to DNA interstrand crosslink (ICL)-inducing agents. It was reported that variants (MIM: 609644) might cause or oligospermia. However, there still lack evidence explain association between different and infertility phenotypes. Herein, we identified...
Abstract Background Non-obstructive azoospermia (NOA) is the most severe disease in male infertility, but genetic causes for majority of NOA remain unknown. Methods Two Chinese NOA-affected patients were recruited to identify causal factor infertility. Whole-exome sequencing (WES) was conducted two with NOA. Sanger and CNV array used ascertain WES results. Hematoxylin eosin (H&E) staining immunofluorescence (IF) carried out evaluate stage spermatogenesis arrested affected cases. Results...
Vasoepididymostomy (VE) is an important surgical treatment to achieve natural conception for patients with obstructive azoospermia (OA), and only unilateral VE can be performed under certain conditions, such as OA congenital absence of the vas deferens (CUAVD) some acquired OA. There a lack relevant reports assessing clinical outcomes in different causes. This study aimed describe features evaluate treatments single-armed patients. From December 2015 June 2021, 46 (including 13...
We found seminal B7-H3 was associated with human sperm concentration. However, the mechanism is unclear. The purpose of this study to investigate expression in mouse testis and determine effects on proliferation spermatogonial stem cells (SSCs) underlying mechanisms.B7-H3 mice at different ages (3 weeks, 8 4 months 9 months) detected by western blot immunohistochemistry. CCK-8 were used measure SSCs after incubation concentrations for 1-72 h vitro. Flow cytometry analyze cell cycle 48 72 h....
Short bowel syndrome (SBS) is featured by impaired nutrients and fluids absorption due to massive small intestine resection. Gut dysbiosis has been implicated in SBS, this study aimed characterize the metagenomic metabolomic profiles of SBS identify potential therapeutic targets.Fecal samples from Sham rats (n = 8 per group) were collected for high-throughput sequencing. Fecal metabolomics was measured untargeted liquid chromatography-mass spectrometry.We found that species-level α-diversity...