A5077371341- Polyomavirus and related diseases
- Full-Duplex Wireless Communications
- Plant Virus Research Studies
- Energy Harvesting in Wireless Networks
- Parvovirus B19 Infection Studies
- RNA Interference and Gene Delivery
- Respiratory viral infections research
- Influenza Virus Research Studies
- Mosquito-borne diseases and control
- Antenna Design and Analysis
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- RNA regulation and disease
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- HIV Research and Treatment
- Multiple Sclerosis Research Studies
- Plant Disease Resistance and Genetics
- Virology and Viral Diseases
- PARP inhibition in cancer therapy
- Viral gastroenteritis research and epidemiology
- Malaria Research and Control
- Cancer therapeutics and mechanisms
- Cytomegalovirus and herpesvirus research
- Food Quality and Safety Studies
Hokkaido University
1995-1998
ABSTRACT Two polyomaviruses, BK virus (BKV) and JC (JCV), are ubiquitous in the human population, generally infecting children asymptomatically then persisting renal tissue. It is thought that reactivation leads to productive infection for both viruses, with progeny shed urine. Several studies have shown rate of viruria increases age host, but a systematic approach examine shedding BKV has not been developed. To elucidate relationship between host age, we obtained urine from donors (healthy...
We established small interfering RNA (siRNA) directed against poly(ADP-ribose) polymerase 1 (PARP-1) that effectively reduces the expression of PARP-1 in two human cell lines. Established siRNA significantly suppressed immunodeficiency virus type (HIV-1) replication, as well activation integrated HIV-1 long terminal repeat promoter. These results indicate is required for efficient replication cells. propose may serve a cellular target interference-mediated gene silencing to inhibit replication.
Progressive multifocal leukoencephalopathy (PML) is a devastating demyelinating disease caused by JC virus (JCV), predominantly affecting patients with impaired cellular immunity. PML non-reportable few exceptions, making national surveillance difficult. In Japan, polymerase chain reaction (PCR) testing for JCV in the cerebrospinal fluid (CSF) performed at National Institute of Infectious Diseases to support diagnosis. To clarify overall profile patient data provided time CSF-JCV over 10...
We describe a case of 67-year-old man with systemic lupus erythematosus who presented progressive left hemiplegia. Although the cerebral spinal fluid (CSF) polymerase chain reaction (PCR) for JC virus was negative, brain biopsy confirmed diagnosis multifocal leukoencephalopathy (PML). The tapering prednisone and use cidofovir could not arrest disease progression. Administration mefloquine stopped extension lesion, resulted in obvious clinical improvement. CSF nested PCR also became negative....
Abstract This report concerns a carrier cell culture (designated JCI) infected persistently with JC virus (JCV). Immunostaining an anti‐JCV antiserum revealed that JCI was in which only small fraction of the cells (‐1.5%) produced virus. The JCV titre increased strikingly by incubating confluent for 4‐6 days medium containing low concentration fetal bovine serum (2%). Viral genomes cloned from were heterogeneous respect to size, but most clones had alteration same regulatory region CR‐JCI)....
Human polyomavirus, JCV, causes fatal demyelinating disease, progressive multifocal leukoencephalopathy (PML). It has been shown that 5HT(2A)R acts as a cellular receptor for JCV on human glial cells. In the current study, we examined inhibitory effects of antagonists, ketanserin and ritanserin, both infection propagation by using neuroblastoma cells IMR-32 JCI, which continuously produce JCV. Transcriptional analysis revealed was constitutively expressed in JCI Treatments with antagonists...
BK polyomavirus (BKPyV) is ubiquitous in human populations, infecting children without obvious symptoms and persisting the kidney. BKPyV isolates have been classified into four subtypes (I–IV) using either serological or genotyping methods. In general, subtype I occurs most frequently, followed by IV, with II III rarely detected. As differences growth capacity cells possibly determine proportion of here properties representative strains as IV renal proximal tubule epithelial (HPTE cells)...
An outbreak of group A rotavirus infection resulted in gastroenteritis among disabled adults an isolated rehabilitation institution Kobe, Japan. Of the 95 residents, 16 were diagnosed with illness. The causative agent was a single strain typical human belonging to VP7 serotype G2, VP4 genotype P[4], and NSP4 A. Mean duration stay significantly longer for residents illness (22.1±11.8 years) than without disease (13.5±10.6 years; P=0.01). Age, sex, disability location resident rooms displayed...
Abstract The incidence of progressive multifocal leukoencephalopathy (PML) has increased due to the AIDS pandemic, hematological malignancies, and immunosuppressive therapies. Recently, number cases monoclonal antibody‐associated PML in patients treated with immunomodulatory drugs such as natalizumab. However, no common consensus regarding therapy been reached clinical studies. In order examine suppression JC virus (JCV) replication by 3‐aminobenzamide (3‐AB), a representative PARP‐1...
JC polyomavirus (JCPyV) causes progressive multifocal leukoencephalopathy (PML), a fatal demyelinating disease of the central nervous system, in immunocompromised patients. Because no drugs have been approved for treating PML, many antiviral agents are currently being investigated this purpose. The inhibitory effects topoisomerase I inhibitors topotecan and β-lapachone were assessed by investigating viral replication, propagation protein 1 (VP1) production cultured cells. JCPyV replication...
ABSTRACT Influenza B virus Yamagata group strains, isolated in the 2000 to 2001 influenza epidemic season, reacted poorly polyclonal ferret sera prepared against strains earlier. The results of genetic analysis clarified that a point mutation nucleotide at position 126 HA1 region and corresponding one-amino-acid substitution altered viral antigenicity.
The purpose of this study is to reevaluate the sensitivities different methods used in diagnosis measles including virus isolation, RT-PCR, and measurement IgM. Sixty-three throat swabs, 84 peripheral blood mononuclear cell (PBMC) samples, 85 plasma samples were collected from cases suspected measles. sensitivity isolation using swabs PBMC comparison with RT-PCR was 58.1 93.5%, respectively. We defined laboratory-confirmed as those which at least one positive. percentage positive results by...
To clarify the stability of BK polyomavirus (BKPyV) genome in renal transplant (RT) recipients, three to five complete BKPyV genomes from each six RT recipients with surviving allografts were molecularly cloned. The sequences these clones determined and compared patient. No nucleotide difference was detected among two patients, a few variations found those four patients. In patients parental sequence (usually major sequence), which variant minor sequences) substitutions would have been...
The role of the autophagy adaptor protein p62/SQSTM1 in Japanese encephalitis virus (JEV) replication mouse embryonic fibroblasts (MEFs) was investigated. Amounts JEV RNA and E were significantly smaller p62-deficient cells than wild-type at 24 hr post-infection (p.i.). quantitation viral plaque assays showed significant reductions titers cell culture fluid. Our results indicate that is impaired MEFs, suggesting p62 positively regulates host cells.
Pathogenic JCV with rearranged regulatory regions (PML-type) causes PML, a demyelinating disease, in the brains of immunocompromised patients. On other hand, archetype persistently infecting kidney is thought to be converted PML-type virus during replication infected host under immunosuppressed conditions. In addition, Tat protein, encoded by HIV-1, markedly enhances expression reporter gene control late promoter. order examine influence on propagation, we used kidney-derived COS-7 cells,...
Abstract The high incidence of progressive multifocal leukoencephalopathy (PML) among individuals with acquired immunodeficiency syndrome (AIDS) is similar to the other immunocompromised diseases. pathogenic JC virus (JCV) rearranged regulatory regions (PML‐type) causes PML, a demyelinating disease in brains patients. In previous study, Tat protein, encoded by human type 1 (HIV‐1), markedly enhanced expression reporter gene under control JCV late promoter. order examine enhancement...
The high incidence of progressive multifocal leukoencephalopathy (PML) in AIDS patients compared with many other immunosuppressive diseases suggests that HIV-1 infection is strictly related to the activation JC virus (JCV) propagation. In this report, propagation PML-type JCV COS-7-derived cell lines stably expressing Tat (COS-tat cells) has been examined. COS-tat cells, production viral particles and replication genomic DNA were markedly increased COS-7 as judged by HA real-time PCR...
As the coverage rate of measles vaccine increases, not all patients present typical symptoms after exposure to virus (MV). The loads in clinical specimens from with vaccine-modified non-typical are expected be low compared those primary MV infection. A rapid and sensitive laboratory procedure is required for diagnosis measles.SYBR Green (TaKaRa) TaqMan (ABI) real-time reverse transcription-polymerase chain reaction (RT-PCR) assays were developed detect MV-RNA. For RT-PCR, primer sets...
Two kinds of tetravalent double-headed sialo-glycosides with short/long spacers between the Neu5Acα2,6Galβ1,4GlcNAc unit and ethylene glycol tetraacetic acid (EGTA) scaffold were found to be capable binding virus-like particles Merkel cell polyomavirus (MCPyV-LP). The process time course interaction ligand MCPyV-LP assessed by dynamic light scattering (DLS). On addition increasing concentrations MCPyV-LP, larger cross-linked aggregates formed until a maximum size was reached. stronger for...