A5082416268- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Protein Degradation and Inhibitors
- Chromatin Remodeling and Cancer
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
University of Utah
2019-2021
Huntsman Cancer Institute
2019-2021
Howard Hughes Medical Institute
2019-2021
Indian Institute of Science Bangalore
2014
Indian Institute of Science Education and Research, Bhopal
2013
The architecture of the RSC complex is a Snf2-family chromatin remodeler that controls promoter most genes in yeast. Using single-particle cryo–electron microscopy, Ye et al. determined structure bound to nucleosome. reveals modular RSC, shows how engages nucleosome, and explains remodeling directionality. strong similarities homologous human complexes are frequently mutated cancers, this provides valuable information for understanding these systems. Science , issue p. 838
Clipping of histone tails has been reported in several organisms. However, the significance and regulation tail clipping largely remains unclear. According to recent discoveries H3 found be involved gene expression chromatin dynamics. Earlier we had provided evidence tissue-specific proteolytic processing White Leghorn chicken liver nuclei. In this study identify a novel activity glutamate dehydrogenase (GDH) as H3-specific protease tissue. This is regulated by divalent ions thiol-disulfide...
SWI/SNF-family remodelers (BAF/PBAF in mammals) are essential chromatin regulators, and mutations human BAF/PBAF components associated with ∼20% of cancers. Cancer-associated missense BRG1 (encoding the catalytic ATPase) have been characterized previously as conferring loss-of-function. Here, we show that cancer-associated BRG1, when placed into orthologous Sth1 ATPase yeast RSC remodeler, separate two categories: loss-of-function enzymes, or instead, gain-of-function enzymes greatly improve...
Gene expression is a multi-step process which requires recruitment of several factors to promoters. One the factors, Sen1p an RNA/DNA helicase implicated in transcriptional termination and RNA processing yeast. In present study, we have identified novel function that regulates ribonucleotide reductase RNR1 gene, essential for maintaining genomic integrity. Cells with mutation domain or lacking N-terminal displayed drastic decrease basal level transcription gene showed enhanced sensitivity...
ABSTRACT Canonical BAF (CBAF) is an essential 12-protein chromatin-remodeling complex that slides and/or ejects nucleosomes using the alternative catalytic ATP-dependent DNA translocases BRG1 or BRM. Currently, regulation of BRG1/BRM activity and nucleosome ejection remain incompletely understood. To address this, we developed a system for full CBAF reconstitution purification, created novel assay. ARID1A DPF2 were dispensable assembly chromatin remodeling activity, contrasting with prior...