A5102987410- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- Alzheimer's disease research and treatments
- RNA Research and Splicing
- Parkinson's Disease Mechanisms and Treatments
- RNA modifications and cancer
- Trace Elements in Health
- Plant tissue culture and regeneration
- Cancer-related gene regulation
- Protein Degradation and Inhibitors
- Plant-Microbe Interactions and Immunity
- Protein Interaction Studies and Fluorescence Analysis
- DNA Repair Mechanisms
- Machine Learning in Bioinformatics
- Plant Parasitism and Resistance
King Abdullah University of Science and Technology
2020-2024
Kootenay Association for Science & Technology
2024
Indian Institute of Science Education and Research, Bhopal
2013-2016
Clipping of histone tails has been reported in several organisms. However, the significance and regulation tail clipping largely remains unclear. According to recent discoveries H3 found be involved gene expression chromatin dynamics. Earlier we had provided evidence tissue-specific proteolytic processing White Leghorn chicken liver nuclei. In this study identify a novel activity glutamate dehydrogenase (GDH) as H3-specific protease tissue. This is regulated by divalent ions thiol-disulfide...
UHRF1 is an important epigenetic regulator associated with apoptosis and tumour development. It a multidomain protein that integrates readout of different histone modification states DNA methylation enzymatic ubiquitylation activity. Emerging evidence indicates the chromatin-binding modules do not act in isolation but interplay coordinated regulated manner. Here, we compared two splicing variants (V1, V2) murine (mUHRF1) human (hUHRF1). We show insertion nine amino acids linker region...
Glutamate dehydrogenase has been recently identified as a tissue-specific histone H3-specific clipping enzyme. We have previously shown that it cleaves free well chromatin-bound H3. However, the physiological significance of this enzyme is still not clear. The present study aimed to improve our understanding its in vivo. Using biochemical and cell biological approaches, we show glutamate primarily associated with euchromatin, re-localizes from nuclear periphery nucleolus upon DNA damage....
Metal ions present in cellular microenvironment have been implicated as drivers of aggregation amyloid forming proteins. Zinc (Zn2+) reported to directly interact with α-synuclein (AS), a causative agent Parkinson's disease and other neurodegenerative diseases, promote its aggregation. AS is small intrinsically disordered protein (IDP) i.e., understanding molecular factors that drive misfolding has challenging since methods used routinely study structure are not effective for IDPs. Here, we...
Chromatin marks are recognized by distinct binding modules, many of which embedded in multidomain proteins. How the different functionalities such complex chromatin modulators regulated is often unclear. Here, we delineated interplay H3 amino terminus– and K9me-binding activities hUHRF1 protein. We show that phosphoinositide PI5P interacts simultaneously with two distant flexible linker regions connecting domains hUHRF1. The dependent on both, polar head group, acyl part phospholipid induces...
Proteolytic clipping of histone H3 has been identified in many organisms. Despite several studies, the mechanism clipping, substrate specificity, and significance this poorly understood epigenetic are not clear. We have previously reported specific proteolytic a protein inhibitor chicken liver. However, sites still known very well. In study, we attempt to identify determine inhibition by stefin B protein, cysteine protease inhibitor. By employing site-directed mutagenesis vitro biochemical...
Abstract In Arabidopsis thaliana , the nuclear protein DEK2 orchestrates diverse chromatin-related processes and exhibits phosphorylation in response to flagellin22 treatment, implicating its involvement plant immunity against bacterial pathogens. Loss-of-function mutants of dek2 have their compromised both fungal Transcriptomic analysis dek2-1 mutant unveils AtDEK2 as a transcriptional repressor defense-related genes, well genes associated with hormone synthesis signaling. Chromatin...
ABSTRACT Metal ions present in cellular microenvironment have been implicated as drivers of aggregation amyloid forming proteins. Zinc (Zn 2+ ) reported to directly interact with α-synuclein (AS), a causative agent Parkinson’s disease and other neurodegenerative diseases, promote its aggregation. AS is small intrinsically disordered protein (IDP) i.e., understanding molecular factors that drive misfolding has challenging since methods used routinely study structure are not effective for...
Abstract Chromatin marks are recognized by distinct binding modules many of which embedded in multidomain proteins or complexes. How the different protein functionalities complex chromatin modulators regulated is often unclear. Using a combination biochemical, biophysical and structural approaches we delineated regulation H3unmodified H3K9me activities UHRF1 protein. The phosphoinositide PI5P interacts with two distant flexible linker regions mode that dependent on polar head group acyl part...