A5003468584- Veterinary Oncology Research
- Infectious Diseases and Mycology
- Virus-based gene therapy research
- Tumors and Oncological Cases
- Cancer Genomics and Diagnostics
- Hedgehog Signaling Pathway Studies
- Alzheimer's disease research and treatments
- Mast cells and histamine
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Genomics and Rare Diseases
- Animal Genetics and Reproduction
- Oral and Maxillofacial Pathology
- Immunotherapy and Immune Responses
- Genomic variations and chromosomal abnormalities
- Molecular Biology Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Genetic factors in colorectal cancer
- Agriculture and Farm Safety
- Chromatin Remodeling and Cancer
- Gene expression and cancer classification
- Ocular Oncology and Treatments
- Congenital heart defects research
- Cardiovascular Function and Risk Factors
- Genetic and phenotypic traits in livestock
Wellcome Sanger Institute
2023-2024
Animal Health Trust
2011-2021
Minnesota Department of Agriculture
2018
Molecular Oncology (United States)
2014
Medical Research Council
2006
Genomics (United Kingdom)
2006
Wellcome Trust
2006
Abstract The Catalogue Of Somatic Mutations In Cancer (COSMIC), https://cancer.sanger.ac.uk/cosmic, is an expert-curated knowledgebase providing data on somatic variants in cancer, supported by a comprehensive suite of tools for interpreting genomic data, discerning the impact alterations disease, and facilitating translational research. catalogue accessed used thousands cancer researchers clinicians daily, allowing them to quickly access information from immense pool curated over 29...
Canine osteosarcoma is clinically nearly identical to the human disease, but common and highly heritable, making genetic dissection feasible.Through genome-wide association analyses in three breeds (greyhounds, Rottweilers, Irish wolfhounds), we identify 33 inherited risk loci explaining 55% 85% of phenotype variance each breed. The greyhound locus exhibiting strongest association, located 150 kilobases upstream genes CDKN2A/B, also most rearranged canine tumors. top germline candidate...
Canine mast cell tumours (CMCT) are one of the most common skin in dogs with a major impact on canine health. Certain breeds have higher risk developing tumours, suggesting that underlying predisposing germ-line genetic factors play role development this disease. The largely unknown, although somatic mutations oncogene C-KIT been detected proportion CMCT, making CMCT comparative model for mastocytosis humans where frequent. We performed genome wide association study golden retrievers from...
Canine mammary tumours (CMT) are the most common neoplasia in unspayed female dogs. CMTs suitable naturally occurring models for human breast cancer and share many characteristics, indicating that genetic causes could also be shared. We have performed a genome-wide association study (GWAS) English Springer Spaniel dogs identified significant locus on chromosome 11 (praw = 5.6x10-7, pperm 0.019). The associated haplotype spans 446 kb region overlapping CDK5RAP2 gene. protein has function cell...
Objective To evaluate if 14 genes that discriminate metastasising and non‐metastasising human uveal melanomas can differentiate in dogs. Methods Nineteen archival biopsies of eyes with a histopathological classification primary benign (n = 9) malignant 10) melanoma were selected. Thoracic and/or abdominal metastases confirmed metastatic spread the tumour seven dogs during follow‐up period. Gene expression was assayed by Reverse Transcription‐quantitative Polymerase Chain Reaction. Genes...
Uveal melanoma (UM) is the most common primary intraocular tumour in dogs. There no effective means of predicting whether a will metastasize. microRNA (miRNA) metastasis signatures have been identified for several human cancers, including UM.In this study we investigated metastasizing and non-metastasizing canine UMs can be distinguished by miRNA expression levels.miRNA microarray profiling was used to compare 8 12 formalin-fixed, paraffin-embedded (FFPE) UM biopsies.Fourteen miRNAs...
Oral malignant melanoma (OMM) is the most common canine melanocytic neoplasm. Overlap between somatic mutation profiles of OMM and human mucosal melanomas suggest a shared UV-independent molecular aetiology. In with melanomas, metastasise. There no reliable means predicting metastasis, systemic therapies for metastatic disease are largely palliative. Herein, we employed exon microarrays comparative expression profiling FFPE biopsies 18 primary that metastasised 10 did not Genes displaying...
Cutaneous mast cell tumours are one of the most common canine cancers. Approximately 25% metastasise. Activating c-kit mutations present in about 20% tumours, but metastases occur absence mutations. Tumour metastasis is associated with significantly diminished survival spite adjuvant chemotherapy. Available prognostic tests do not reliably predict whether a tumour will In this study we compared global expression profiles 20 primary cutaneous that metastasised those did The objective was to...
Dogs represent a unique spontaneous cancer model. Osteosarcoma (OSA) is the most common primary bone tumor in dogs (OMIA 001441-9615), and strongly resembles human forms of OSA. Several large- to giant-sized dog breeds, including Leonberger, have greatly increased risk developing We performed genome-wide association analysis with high-density imputed SNP genotype data from 273 Leonberger cases median age 8.1 [3.1-13.5] years 365 controls older than eight years. This revealed significant...
Mast cell tumours are the most common type of skin cancer in dogs, representing a significant concern canine health. The molecular pathogenesis is largely unknown, but breed-predisposition for mast tumour development suggests involvement inherited genetic risk factors some breeds. In this study, we aimed to identify germline associated with Labrador Retrievers, breed an elevated development. Using methodological approach that combined genome-wide association targeted next generation...
Objectives To describe the clinicopathological and genetic characteristics of mast cell tumours in dogs less than 12 months old. Materials Methods Retrospective review aged when diagnosed with at three referral hospitals UK. Results Sixteen pure‐bred were included, which 11 female. The median age first presentation diagnosis 7.6 9 months, respectively. In 13 cutaneous they subcutaneous. Four described as high‐grade (Patnaik or Kiupel) nine Patnaik grade II; had mitotic index >5 10...
High immigration rates contribute to the high incidence of pediatric tuberculosis (TB) in San Diego, Calif. Adolescents frequently have poor access health care and may not receive appropriate TB screening. School-based screening has been ineffective detecting other parts country.To determine prevalence infection disease a high-risk population school students through school-based screening.Cross-sectional study an analysis risk factors for attending 2 Diego schools with percentages...
Cytogenetic detection of unbalanced genomic aberrations in tumours is a strategy for the identification tumour suppressor genes and oncogenes. When considered concert with clinical data, approach also represents means identifying markers prognosis. In preliminary investigation molecular basis canine meningioma tumorigenesis, we profiled three by comparative hybridization. Distinct patterns sub-chromosomal deletions were identified suggesting alternative mechanisms initiation. The deleted...
Conventional classification schemes for canine lymphomas do not discriminate between phenotypically indistinct tumours that may exhibit differences in behaviour. Transcriptional profiling has the potential to afford objective clinically relevant stratification of lymphomas, and its sensitivity means prognostic assays could be performed on tumour needle aspirates collected without anaesthesia. In this pilot study, we compared expression profiles derived from surgical biopsies fine five...
To evaluate the effect of syringe size, needle number passes and operator experience on cell yield from tumour fine-needle aspirates, quantity quality extractable RNA.Fine-needle aspirates were collected canine lymphoma, cutaneous mast tumour, anal gland adenocarcinoma, fibrosarcoma oral malignant melanoma using nine different techniques.There was a significant difference in between aspirate techniques for melanoma, lymphoma adenocarcinoma. The application suction yielded largest cells. Cell...
To document the fine needle aspiration methods used by UK veterinary practitioners for assessment of cutaneous masses and relate this to achievement a representative sample.An internet-based questionnaire was designed publicised in national press, at surgical meeting, letters surgeons.One hundred seventy respondents replied questionnaire: 58 · 2% sampled on basis appearance or behaviour; 41 3% every mass. Practitioners with greater oncological caseload who graduated more recently were likely...
The identification of individual dog chromosomes is problematic because the 38 pairs autosomes are small and acrocentric. Here we describe design application a FISH tool that enables definitive each autosome in normal karyotype, without relying on subjective interpretation DAPI banding patterns. From high-resolution physical map canine genome, have chosen panel 80 chromosome-specific BAC clones. DNA from clone labeled with one five different fluorochrome-conjugated nucleotides. By selecting...