A diverse range of Pd(II)-catalyzed enantioselective C-H activation reactions have been developed to construct point, axial, and planar chirality. Despite the importance chiral biaryl scaffolds in bioactive molecules ligands, atroposelective functionalization at meta-position remains a significant challenge. Here, we realized rare remote meta-C-H with monoprotected amino acid (MPAA) ligand racemic transient norbornene mediator. The reaction starts ortho-C-H via kinetic resolution give...

To describe detailed behaviour of cell spreading under the influence substrate stiffness, A549 cells cultured on surfaces polydimethylsiloxane (PDMS) and polyacrylamide (PAAm) with bulk rigidities ranging from 0.1 kPa to 40 were in situ observed. The PAAm presented a positive correlation between speed stiffness. After computing deformations gels collagen, stiffness PAAm, rather than matrix tethering, determined behaviour. On other hand, was unaffected by varying PDMS. Based simulation...

Abstract Background Biosynthesis of fatty alk(a/e)ne in cyanobacteria has been considered as a potential basis for the sunlight-driven and carbon-neutral bioprocess producing advanced solar biofuels. Aldehyde-deformylating oxygenase (ADO) is key enzyme involved that pathway. The heterologous or chemical reducing systems were generally used vitro ADO activity assay. cognate electron transfer system from to support still unknown. Results We identified endogenous including ferredoxin (Fd)...

The fatty alk(a/e)ne biosynthesis pathway found in cyanobacteria gained tremendous attention recent years as a promising alternative approach for biofuel production. Cyanobacterial aldehyde-deformylating oxygenase (cADO), which catalyzes the conversion of Cn aldehyde to its corresponding Cn-1 alk(a/e)ne, is key enzyme that pathway. Due low activity, production by cADO an inefficient process. Previous biochemical and structural investigations have provided some information on catalytic...

Abstract Objectives To appraise effective predictors for infection in patients with decompensated cirrhosis (DC) by using XGBoost algorithm a retrospective case-control study. Methods Clinical data were retrospectively collected from 6,648 DC admitted to five tertiary hospitals. Indicators significant differences determined univariate analysis and least absolute contraction selection operator (LASSO) regression. Further multi-tree extreme gradient boosting (XGBoost) machine learning-based...

Abstract Ca 2+ -sensing receptor (CaSR) represents a potential therapeutic target for inflammatory bowel diseases and strongly prefers aromatic amino acid ligands. We investigated the regulatory effects of dietary supplementation with acids – tryptophan, phenylalanine tyrosine (TPT) on CaSR signalling pathway intestinal response. The in vivo study was conducted weanling piglets using 2 × factorial arrangement randomised complete block design. Piglets were fed basal diet or supplemented TPT...

Weaning stress may cause reduced energy intake for maintenance of mucosal structure. Gln, Glu, and Asp are major sources the small intestine. This study investigated whether improve intestinal morphology via regulating metabolism in weaning piglets. A total 198 weaned piglets were assigned to 3 treatments: Control (Basal diet + 1.59% L-Ala); T1 1% L-Gln 0.5% L-Glu 0.1% L-Asp); T2 (Low L-Asp). Jejunum ileum obtained on d 5 or 21 post-weaning. enhanced growth performance. treatments improved...

The study was to investigate the effect of early-weaning stress and proline (Pro) putrescine (Put) supplementations on serum biochemical parameters amino acids (AA) metabolism in suckling post-weaning pigs. Blood small intestinal mucosa were harvested from piglets at 1, 7, 14, 21 d age 3, 5, 7 after weaning 14 age, as well received oral administration Pro Put 1 old. In piglets, glucose, albumin total cholesterol levels increased (

Dietary supplementation with aromatic amino acids (AAAs) has been demonstrated to alleviate intestinal inflammation induced by lipopolysaccharide (LPS) in the piglets. But mechanism of AAA sensing and utilization under inflammatory conditions is not well-understood. The study was conducted 32 weanling piglets using a 2 × factorial arrangement (diet LPS challenge) randomized complete block design. Piglets were fed as basal diet or supplemented 0.16% tryptophan (Trp), 0.41% phenylalanine...

Summary HIV-syphilis co-infection is often cited as a major reason behind recent resurgence in syphilis prevalence among men who have sex with China. Most published literatures explore factors associated either HIV or syphilis, but not their co-infection. We analysed data from cross-sectional survey on seven Chinese cities. Snowball sampling was used to recruit participants for the survey. Socio-demographic and behavioural predictors mono/co-infection were examined using ordinal logistic...

Self-sufficient aldehyde deformylating oxygenases (ADOs) from Synechococcus elongatus PCC7942 fused to alternative electron transfer systems were successfully designed, constructed, characterized and used for efficient conversion of aldehydes into alkanes the first time.

Functional connectivity studies based on region of interest approach suggest altered functional the default mode network (DMN), executive control (ECN), and salience (SN). The aim this study is to determine whether intranetwork internetwork brain are in both post-traumatic stress disorder (PTSD) patients traumatized subjects without PTSD using a data-driven approach.Resting-state MRI data were acquired for 27 with typhoon-related PTSD, 33 trauma-exposed controls (TEC), 30 healthy (HC)....

A previous study has demonstrated that early weaning significantly suppressed hepatic glucose metabolism in piglets. Glutamate (Glu), aspartate (Asp) and glutamine (Gln) are major metabolic fuels for the small intestine can alleviate stress, therefore might improve energy metabolism. The objective of this was to investigate effects administration Glu, Asp Gln on expression genes proteins involved lipid post-weaning Thirty-six weaned piglets were assigned following treatments: control diet...

Abstract Postnatal growth retardation (PGR) is common in piglets. Abnormal development small intestine was casually implicated impaired growth, but the exact mechanism still implausible. The present study unveiled transcriptome profile of jejunal mucosa, major site nutrient absorption, PGR and healthy piglets using RNA-sequencing (RNA-seq). middle segments jejunum ileum, mucosa were obtained from at 42 d age. Total RNA samples extracted submitted for RNA-seq. Lower villus height observed...

Abstract Individuals with postnatal growth retardation (PGR) are prone to developing chronic diseases. Abnormal development in small intestine is casually implicated impaired growth. However, the exact mechanism still implausible. In this present study, PGR piglets (aged 42 days) were employed as a good model analyze developmental changes intestinal mucosal barrier function. Our data demonstrated that exhibited jejunal and ileal epithelial villous morphology permeability, accompanied by...

We investigated the effects of rapamycin (RAPA) and chloroquine (CQ) in supporting growth performance intestinal mucosal barrier response to deoxynivalenol (DON) piglets. A total 32 healthy weaned piglets (bodyweight <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mn>7.10</mml:mn><mml:mo>±</mml:mo><mml:mn>0.58</mml:mn><mml:mtext> </mml:mtext><mml:mtext>kg</mml:mtext></mml:math>) were divided into four groups treated daily with RAPA (1 mg/kg BW), CQ (10 or a control...

An efficient copper-catalyzed multicomponent reaction of phenols, amides, and 4-bromphenyl iodide was developed that uses commercially available N,N-dimethylglycine as the ligand. This proceeds in moderate to good yields for a range phenols amides. The simple experimental procedure high levels functional group compatibility make this method attractive applications on pesticides.

Aspartate (asp), glutamate (glu), and glutamine (gln) are the major energy fuels for small intestine, it had been indicated in our previous study that mix of these three amino acid supplementations could maintain intestinal homeostasis. This aimed to further investigate whether treatment gln, glu, asp low diet affects barrier integrity pool weaning piglets. A total 198 weaned piglets were assigned 3 treatments: control (basal + 1.59% L-Ala); T1 1% L-Gln 0.5% L-Glu 0.1% L-Asp); T2 (low...

Abstract Background Helicobacter pylori ( H ) immunoglobulin G (IgG) seropositivity is prevalent but its relation with leukocyte telomere length (LTL), a cellular aging biomarker, unclear. Methods Among 3,472 participants from the National Health and Nutrition Examination Survey (NHANES) cycle 1999‐2000, LTL was measured quantitative polymerase chain reaction. IgG by enzyme‐linked immunosorbent assays defined as an immune status ratio score &gt; 0.9. We used linear regression models to...

l-proline (Pro) is a precursor of ornithine, which converted into polyamines via ornithine decarboxylase (ODC). Polyamines plays key role in the proliferation intestinal epithelial cells. The study investigated effect Pro on polyamine metabolism and cell porcine enterocytes vivo vitro. Twenty-four Huanjiang mini-pigs were randomly assigned 1 3 groups fed basal diet that contained 0.77% alanine (Ala, iso-nitrogenous control), 1% or + 0.0167% α-difluoromethylornithine (DFMO) from d 15 to 70...