The therapeutic effect of induced pluripotent stem cells (iPSs) on the progression chronic kidney disease (CKD) has not yet been demonstrated. In this study, we sought to assess whether treatment with iPSs retards CKD when compared bone marrow mesenchymal (BMSCs). Untreated 5/6 nephrectomized rats were animals receiving BMSCs or iPSs. Renal function, histology, immunohistochemistry, and gene expression studied. Implanted tracked by SRY analysis. Both treatments minimized elevation in serum...

Mutations and alterations in the expression of VEGFA, KRAS, NFE2L2 oncogenes play a key role cancer initiation progression. These genes are enrolled not only cell proliferation control, but also angiogenesis, drug resistance, metastasis, survival tumor cells. MicroRNAs (miRNAs) small, non-coding regulatory RNA molecules that can regulate post-transcriptional multiple target genes. We aimed to investigate if miRNAs hsa-miR-17-5p, hsa-miR-140-5p, hsa-miR-874-3p could interfere lines derived...

OBJETIVOS: Implantação de técnicas isolamento e cultivo células-tronco mesenquimais do sangue cordão umbilical humano, com sem uso gradiente densidade Ficoll-Paque (d=1,077g/ml). MÉTODOS: Dez amostras humano gestação a termo foram submetidas dois procedimentos mesenquimais: densidade. As células semeadas em frascos 25cm² uma 1x10(7)células nucleadas/cm² (sem Ficoll) 1,0x10(6) mononucleares/cm² (com Ficoll). aderentes marcação citoquímica fosfatase ácida reativo Schiff. RESULTADOS: No...

Different routes for the administration of bone marrow-derived cells (BMDC) have been proposed to treat progression chronic renal failure (CRF). We investigated whether (1) use bovine pericardium (BP) as a scaffold cell therapy would retard CRF and (2) efficacy differently impacts distinct degrees CRF. used 2/3 5/6 models mass reduction simulate different stages chronicity. Treatments consisted BP seeded with either mesenchymal or mononuclear implanted in parenchyma remnant kidney. Renal...

Differential expression of genes encoding cytochrome P450 (CYP) and other oxygenases enzymes involved in biotransformation mechanisms endogenous exogenous compounds can lead to oral tumor development. We aimed identify the profile these genes, searching for susceptibility biomarkers squamous cell carcinoma. Sixteen carcinoma samples were included this study (eight eight adjacent non-tumor tissues). Gene quantification was performed using TaqMan Array Human CYP450 Oxygenases 96-well plate...

Abstract Biodegradable polymeric microspheres can be used to deliver drugs through controlled rate and targeted processes. The drug is released from the particles by leaching or degradation of matrix. Crystallinity play a very important role in matrixes; it affect drug‐release rate, especially chemoembolization. Most commercial embolic agents have low degree crystallinity, correlation between drug‐delivery crystallinity not fully understood. This study presents appropriated synthesis...

Implantation of cell separation and mesenchymal stem culture techniques from human umbilical cord blood with without using the Ficoll-Paque gradient density method (d=1.077 g/ml).Ten samples obtained full-term deliveries were submitted to two different procedures culture: a) Method gradient, which concentrates all nucleated cells; b) selects only low-density mononuclear cells. Cells initially plated into 25 cm(2) cultures flasks at a 1 x 10(7) cells/cm(2) 10(6) cells/cm(2).It was 2-13...

Cancer biologists have focused on studying cancer stem cells (CSCs) because of their ability to self-renew and recapitulate tumor heterogeneity, which increases resistance chemotherapy is associated with relapse. Here, we used two approaches isolate CSCs: the first involved metabolic enzyme aldehyde dehydrogenase ALDH, second three cell surface markers CD44, CD117, CD133. ALDH showed a higher zinc finger E-box binding homeobox 1 (ZEB1) microRNA (miRNA) expression than CD44/CD117/133...

Cell therapy constitutes a possibility for improving nerve regeneration, increasing the success of repair. We evaluate use mononuclear cells in regeneration sciatic after axotomy followed by end-to-end neurorrhaphy. Forty adult male Wistar rats (250-300 g) were divided into four groups: (1) sham, (2) neurorrhaphy: was sectioned and repaired using epineural sutures, (3) culture medium: suture, received an injection 10 μL medium nerve, (4) cell: concentration 3 × 10(6) cell injected epineurium...

MicroRNAs (miRNAs) are short non-coding RNA molecules acting as important posttranscriptional gene and protein expression regulators in cancer. The study goal was to examine VEGFA (vascular endothelial growth factor A) hepatocellular carcinoma (HCC) cell lines upon transfection miR-612, miR-637, or miR-874. Methods: MiR-612 mimics, miR-637 miR-874 inhibitors were transfected using Lipofectamine RNAiMax both HCC lines, HepG2 HuH-7. Real-time PCR, Western blotting, ELISA methods used evaluate...

We report on a familial t(4;7)(q28;p22) with 2:2 adjacent-1 unbalanced segregation producing duplication of 4q28-->qter in multiple offspring. Within the large four-generation pedigree, carrier had reproductive outcome that was approximately equal for 1) balanced translocation, 2) normal chromosomes, and 3) viable 4q trisomy or pregnancy loss. The three individuals chromosomal confirmation (comprising 1.8% haploid autosomal length) similar mental developmental retardation, hypotonia,...

Caldas, H.; Fernandes, I. M.; Braile, D. Goloni-Bertollo, E.; Kawasaki-Oyama, R. S.; Baptista, M. A.S.; Abbud-Filho, Author Information

We report on a familial t(4;7)(q28;p22) with 2:2 adjacent-1 unbalanced segregation producing duplication of 4q28→qter in multiple offspring. Within the large four-generation pedigree, carrier had reproductive outcome that was approximately equal for 1) balanced translocation, 2) normal chromosomes, and 3) viable 4q trisomy or pregnancy loss. The three individuals chromosomal confirmation (comprising 1.8% haploid autosomal length) similar mental developmental retardation, hypotonia,...

Introdução: O carcinoma hepatocelular (CHC) é o câncer primário de fígado mais frequente, abrangendo 90% dos cânceres hepáticos iniciados no fígado, e 70-80% todos os fígado. Os receptores ativados por proliferadores peroxissoma (PPARs) são nucleares ligantes, que atuam como fatores transcrição, regulando a expressão genes relacionados ao metabolismo glicose lipídios. PPARγ compreende uma das três isoformas PPARs, atuando processo regulação da adipogênese, biossíntese armazenamento As...

Introdução: O câncer é uma doença multifatorial influenciada por fatores ambientais e genéticos. A progressão tumoral, os mecanismos de plasticidade celular o desenvolvimento metástase são coordenados rede complexa alterações genômicas epigenômicas, aspectos que representam um grande desafio para diagnóstico tratamento da doença. fator crescimento endotelial vascular (VEGF) principal responsável pela formação novos vasos sanguíneos a partir pré-existentes, processo denominado angiogênese....