A5044950038- Neonatal Respiratory Health Research
- Asthma and respiratory diseases
- Respiratory viral infections research
- Tracheal and airway disorders
- Cystic Fibrosis Research Advances
- Inhalation and Respiratory Drug Delivery
- Pediatric health and respiratory diseases
- IL-33, ST2, and ILC Pathways
- Respiratory and Cough-Related Research
- Barrier Structure and Function Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Neuroscience of respiration and sleep
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Congenital Diaphragmatic Hernia Studies
- Transplantation: Methods and Outcomes
- Viral gastroenteritis research and epidemiology
- Pharmacological Effects and Assays
- Artificial Immune Systems Applications
- Birth, Development, and Health
- Immune Response and Inflammation
- Vitamin D Research Studies
- Cell Adhesion Molecules Research
- Connexins and lens biology
- Protein purification and stability
- Nasal Surgery and Airway Studies
Curtin University
2020-2025
The University of Western Australia
2014-2025
The Kids Research Institute Australia
2016-2023
Princess Margaret Hospital for Children
2013
Neutrophil elastase is the most significant predictor of bronchiectasis in early-life cystic fibrosis; however, causal link between neutrophil and airway damage not well understood. Matrix metalloproteinases (MMPs) play a crucial role extracellular matrix modelling are activated by elastase. The aim this study was to assess if MMP activation positively correlates with activity, disease severity young children fibrosis.Total MMP-1, MMP-2, MMP-7, MMP-9, tissue inhibitor metalloproteinase...
COVID-19 is complicated by acute lung injury, and death in some individuals. It caused SARS-CoV-2 that requires the ACE2 receptor serine proteases to enter AEC. We determined what factors are associated with expression particularly patients asthma COPD.
Current limitations to primary cell expansion led us test whether airway epithelial cells derived from healthy children and those with asthma cystic fibrosis (CF), co-cultured an irradiated fibroblast feeder in F-medium containing 10 µM ROCK inhibitor could maintain their lineage during this is influenced by underlying disease status. Here, we show that conditionally reprogrammed (CRAECs) can be established both diseased phenotypes. CRAECs expanded, cryopreserved phenotypes over at least 5...
Bronchial epithelial tight junctions (TJ) have been extensively assessed in healthy airway epithelium. However, no studies yet the effect of human rhinovirus (HRV) infection on expression and resultant barrier function childhood asthma.To investigate impact HRV TJ cells (AECs) children with without asthma. Furthermore, to test hypothesis that integrity is compromised a greater extent by AECs from asthmatic children.Primary were obtained asthma, differentiated into air-liquid interface (ALI)...
Summary Background The airway epithelium forms an effective immune and physical barrier that is essential for protecting the lung from potentially harmful inhaled stimuli including viruses. Human rhinovirus ( HRV ) infection a known trigger of asthma exacerbations, although mechanism by which this occurs not fully understood. Objective To explore relationship between apoptotic, innate inflammatory responses to in epithelial cells AEC s) obtained children with non‐asthmatic controls. In...
Apically located tight junctions in airway epithelium perform a fundamental role controlling macromolecule migration through paracellular spaces. Alterations their expression may lead to disruptions barrier integrity, which subsequently facilitates entry of potential bacterial and other pathogens into the host. Furthermore, there is emerging evidence that integrity certain inflammatory diseases be altered. However, little consensus on way this assessed measured type cells used achieve...
No studies have assessed the effects of human rhinovirus (HRV) infection on epithelial tight junctions (TJs) and resultant barrier function.To correlate viral with TJ disassembly, integrity, function.Human airway cells were infected HRV minor serotype 1B (HRV-1B) at various 50% tissue culture infectivity doses (TCID50) over 72 hours. replication was by quantitative-polymerase chain reaction (qPCR) while cell viability apoptosis proliferation apoptotic assays, respectively. Protein expression...
Abnormal wound repair has been observed in the airway epithelium of patients with chronic respiratory diseases, including asthma. Therapies focusing on repairing vulnerable airways, particularly early life, present a potentially novel treatment strategy. We report defective lower epithelial cell to strongly associate common pre–school-aged and school-aged wheezing phenotypes, characterized by aberrant migration patterns reduced integrin α5β1 expression. Next generation sequencing identified...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
Abstract Background and objective Evidence into the role of TGF‐β1 in airway epithelial repair asthma is still controversial. This study tested hypothesis that reduced levels previously observed paediatric asthmatic cells directly contribute to dysregulated seen these cells. Methods Primary (pAEC) from children with ( n = 16) non‐asthmatic subjects 20) were isolated, subcultured for investigation gene protein via quantitative polymerase chain reaction (qPCR) enzyme‐linked immunosorbent assay...
The airway epithelium of children with wheeze is characterized by defective repair that contributes to disease pathobiology. Dysregulation developmental processes controlled Notch has been identified in chronic asthma. However, its role epithelial cells young wheeze, particularly during repair, yet be determined. We hypothesized dysregulated primary (pAEC) contributing repair. This study investigated transcriptional and protein expression function pAEC isolated from without wheeze. Primary...
Background: Output from spacers (or valved holding chambers) is sensitive to changes in breathing pattern. Different have unique characteristics that may influence breathing. A method used for simulation, where the simulated can be recorded on subjects while they are using spacers, allow more accurate vitro estimation of drug delivery specific populations, spacers. Methods: flow chamber was record salbutamol administered two adult through different Each subject performed a series patterns...
Aim of the study: The bronchial brushing technique presents an opportunity to establish a gold standard in vitro model Cystic Fibrosis (CF) airway disease. However, unique obstacles exist when establishing CF epithelial cells (pAECCF). We aimed identify determinants culture success through retrospective analysis program routinely children with CF. Materials and methods: Anaesthetised (CF non-CF) had samples taken which were immediately processed for cell culture. Airway data cohort was...
In this study we assessed the effects of antigen exposure in mice pre-sensitized with allergen following viral infection on changes lung function, cellular responses and tight junction expression. Female BALB/c were sensitized to ovalbumin infected influenza A before receiving a second sensitization challenge saline, (OVA) or house dust mite (HDM). Fifteen days post-infection, bronchoalveolar inflammation, serum antibodies, responsiveness methacholine barrier integrity assessed. There was no...
Dysregulated airway epithelial repair following injury is a proposed mechanism driving posttransplant bronchiolitis obliterans (BO), and its clinical correlate syndrome (BOS). This study compared gene cellular characteristics of in large (LAEC) small (SAEC) cells transplant patients.Subjects were recruited at the time routine bronchoscopy posttransplantation included patients with without BOS. Airway obtained from bronchial bronchiolar brushing performed under radiological guidance these...
Abstract Evidence from animal models demonstrate that intrauterine growth restriction (IUGR) alters airway structure and function which may affect susceptibility to disease. Airway inflammation dysregulated epithelial barrier properties are features of asthma have not been examined in the context IUGR. This study used a maternal hypoxia-induced IUGR mouse model assess lung-specific systemic tight junctions (TJs) protein expression. Pregnant BALB/c mice were housed under hypoxic conditions...
Purchase and disinfection costs together with medication delivery factors may influence the choice of drug options. This study assessed salbutamol habits used in respiratory laboratories quantified delivered dose locally available spacers.An online survey was to obtain data on processes, device choices. The six commercial spacers assessed. Particle size distribution (Ventolin, GSK, 100µg/actuation) from each type measured by quantifying amount (µg) deposited stage an Anderson Cascade...