A5081093460- Fungal Infections and Studies
- Nail Diseases and Treatments
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Beetle Biology and Toxicology Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Antifungal resistance and susceptibility
- Immune Cell Function and Interaction
- Multiple Sclerosis Research Studies
- Healthcare and Venom Research
- Venomous Animal Envenomation and Studies
- Antimicrobial Peptides and Activities
- Galectins and Cancer Biology
- RNA Interference and Gene Delivery
- Malaria Research and Control
- Systemic Lupus Erythematosus Research
- Laser Applications in Dentistry and Medicine
- Viral gastroenteritis research and epidemiology
- Trypanosoma species research and implications
- Neurogenesis and neuroplasticity mechanisms
- Cancer Research and Treatments
- Nerve injury and regeneration
- Research on Leishmaniasis Studies
- Neuroscience and Neuropharmacology Research
- Virus-based gene therapy research
Universidade Estadual de Campinas (UNICAMP)
2014-2024
Hospital de Clínicas da Unicamp
2006-2020
Bipar
1998
Background The modulation of inflammatory processes is a necessary step, mostly orchestrated by regulatory T (Treg) cells and suppressive Dendritic Cells (DCs), to prevent the development deleterious responses autoimmune diseases. Therapies that focused on adoptive transfer Treg or their expansion in vivo achieved great success controlling inflammation several experimental models. Chloroquine (CQ), an anti-malarial drug, was shown reduce inflammation, although mechanisms are still obscure....
The thymus plays a crucial role in the development of T lymphocytes providing an inductive microenvironment which committed progenitors undergo proliferation, T-cell receptor gene rearrangements and thymocyte differentiation into mature T-cells. forms complex network interaction that comprises non lymphoid cells (e.g., thymic epithelial cells, TEC), cytokines, chemokines, extracellular matrix elements (ECM), metalloproteinases other soluble proteins. meshwork is major component...
Galectin-3 (gal-3) is a β-galactoside binding protein related to many tumoral aspects, e.g. angiogenesis, cell growth and motility resistance death. Evidence has shown its upregulation upon hypoxia, common feature in solid tumors such as glioblastoma multiformes (GBM). This tumor presents unique described pseudopalisading cells, which accumulate large amounts of gal-3. Tumor cells far from hypoxic/nutrient deprived areas express little, if any Here, we have that the hybrid glioma line,...
Chloroquine (CQ), an antimalarial drug, has been shown to modulate the immune system and reduce severity of experimental autoimmune encephalomyelitis (EAE). The mechanisms disease suppression are dependent on regulatory T cell induction, although Tregs‐independent exist. We aimed evaluate whether CQ is capable bone marrow‐derived dendritic cells (DCs) both phenotypically functionally as well transfer CQ‐modulated DCs reduces EAE course. Our results show that CQ‐treated presented altered...
Neutrophils (PMN) play a central role in host defense against the neglected fungal infection paracoccidioidomycosis (PCM), which is caused by dimorphic fungus Paracoccidioides brasiliensis (Pb). PCM of major importance, especially Latin America, and its treatment relies on use antifungal drugs. However, course lengthy, leading to side effects even development resistance. The goal study was low-level laser therapy (LLLT) stimulate PMN fight Pb vivo. Swiss mice with subcutaneous air pouches...
Injuries to peripheral nerves cause loss of motor and sensory function, greatly affecting life quality. Successful repair the lesioned nerve requires efficient cell debris removal, followed by axon regeneration reinnervation target organs. Such process is orchestrated several cellular molecular events in which glial immune cells actively participate. It known that tissue clearance largely improved macrophages, activation potentiated molecules acquired system, such as T helper lymphocytes...
Inflammation is a necessary process to control infection. However, exacerbated inflammation, acute or chronic, promotes deleterious effects in the organism. Violacein (viola), quorum sensing metabolite from Gram-negative bacterium Chromobacterium violaceum, has been shown protect mice malaria and have beneficial on tumors. it not known whether this drug possesses anti-inflammatory activity. In study, we investigated viola administration able reduce chronic autoimmune inflammation. For that...
Summary Background and Aims Experimental autoimmune encephalomyelitis (EAE) is T‐cell‐dependent disease of the central nervous system (CNS) mice. This model resembles multiple sclerosis (MS) in many aspects. Therapies that focus modulation immune response cellular infiltration CNS present best effects clinics. Artesunate (Art) a semi‐synthetic sesquiterpene derivative from artemisinin has been shown to reduce clinical signs models through mechanisms not yet understood. In this study, we...
Major histocompatibility complex class one (MHC-I) antigen-presenting molecules participate in central nervous system (CNS) synaptic plasticity, as does the paired immunoglobulin-like receptor B (PirB), an MHC-I ligand that can inhibit immune-cells and bind to myelin axon growth inhibitors. Based on dual roles of both immune systems, we evaluated their expression peripheral (PNS) following sciatic nerve injury mice. Increased PirB protein gene is present spinal cord week after transection,...
A human malignant continuous cell line, named NG97, was recently established in our laboratory. This line has been serially subcultured over 100 times standard culture media presenting no sign of senescence. The NG97 a doubling time about 24 h. Immunocytochemical analysis glial markers demonstrated that cells are positive for fibrillary acidic protein (GFAP) and S-100 protein, negative vimentin. Under phase-contrast microscope, cultures showed with variable morphological features, such as...
Phoneutria nigriventer spider venom (PNV) causes uneven BBB permeability throughout different cerebral regions. Little is known about cellular and molecular responses which course with the PNV-induced opening. We investigate by immunohistochemistry (IHC) Western blotting (WB), GFAP, S100, IFN-gamma TNF-alpha proteins expression in hippocampus cerebellum after time-points from or saline intravenous injection. All variably altered its temporally regionally. WB showed increased GFAP content at...
Molecules from animal venoms are promising candidates for the development of new drugs. Previous in vitro studies have shown that venom spider Phoneutria nigriventer (PnV) is a potential source antineoplastic components with activity glioblastoma (GB) cell lines. In present work, effects PnV on tumor were established vivo using xenogeneic model. Human GB (NG97, most responsive line previous study) cells inoculated (s.c.) back RAG-/- mice. (100 µg/Kg) was administrated every 48 h (i.p.) 14...
Literature has shown that immunosuppression observed in systemic mycosis can be related to damage primary lymphoid organs. We have studied the immunopathological alterations induced experimentally by Paracoccidioides brasiliensis these In this work, thymic BALB/c mice during acute and chronic stages of infection are described. It was P. is able invade microenvironment, inducing severe atrophy characterized degeneration cortical area, organ weight decrease, loss corticomedullary delimitation...
Summary We previously showed alterations in the thymus during experimental infection with Plasmodium berghei . Such comprised histological changes, loss of cortical–medullary limits, and intrathymic presence parasites. As combination chemokines, adhesion molecules extracellular matrix (ECM) is critical to appropriate thymocyte development, we analysed thymic expression ECM ligands receptors, as well chemokines their respective receptors P. infection. Increased components was observed thymi...