A5027469313- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Telomeres, Telomerase, and Senescence
- Craniofacial Disorders and Treatments
- Bone and Dental Protein Studies
- Neuropeptides and Animal Physiology
- Erythrocyte Function and Pathophysiology
- Periodontal Regeneration and Treatments
- Ion channel regulation and function
- Pluripotent Stem Cells Research
- Oral and Maxillofacial Pathology
- Bone health and treatments
- Immune cells in cancer
- Neonatal Respiratory Health Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Osteomyelitis and Bone Disorders Research
- dental development and anomalies
- Dental Radiography and Imaging
- Oral microbiology and periodontitis research
- Electrospun Nanofibers in Biomedical Applications
- Pain Mechanisms and Treatments
- Nerve injury and regeneration
- Oral Health Pathology and Treatment
- Connexins and lens biology
- Extracellular vesicles in disease
Keio University
2014-2025
Tokyo Dental College
2021-2025
Kanagawa Dental University
2024
Harvard University
2021
Harvard Stem Cell Institute
2019
Keio University Hospital
2016-2018
Severe peripheral nerve damage always requires surgical treatment. Autologous transplantation is a standard treatment, but it not sufficient due to length limitations and extended time. Even with the available artificial nerves, there still large room for improvement in their therapeutic effects. Novel treatments injury are greatly expected.
Odontoblasts are terminally differentiated cells that exhibit mechanosensitivity and mineralization capacity. Mechanosensitive ion channels such as Piezo1 present in odontoblasts associated with their physiological functions via Ca 2+ signaling. Both signals influx from mechanosensitive release stores function secondary messenger systems for various biological phenomena. The endoplasmic reticulum (ER) serves an intracellular store mobilizes . Changes concentration inside the ER among factors...
Age-related alveolar bone resorption poses a major dental health challenge, yet its mechanisms and treatments are poorly understood. This study investigates the impact of dasatinib quercetin (D + Q) treatment on senescent cells (SnCs), senescence-associated secretory phenotype (SASP), neutrophil infiltration in aged bone, aiming to develop new strategies for combating age-related resorption. C57BL/6 mice (2 18 months) were used examine resorption, inflammaging, infiltration. Aged received D...
Spindle cell carcinoma (SpCC) is a subtype of poorly differentiated squamous carcinoma, and comprises biphasic mixture epithelial mesenchymal cells. SpCC rare aggressive cancer, with dismal prognosis. Furthermore, its characteristics remain unclear, complicating treatment for this malignancy. Developing specialized strategies that are different from traditional approaches conventional essential obtaining good clinical outcomes. Our previous report documented the presence platelet-derived...
Strategies for therapeutic cell transplantation have been assessed use in the treatment of massive peripheral nerve defects. To support safe and efficient transplantation, we focused on purification cells using surface markers. Our group previously reported low-affinity growth factor receptor (LNGFR)- thymocyte antigen-1 (THY-1)-positive neural crest-like (LT-NCLCs), generated from human induced pluripotent stem (hiPSCs). In present study, investigated efficacy hiPSC-derived LT-NCLCs a...
Odontoblasts play critical roles in dentin formation and sensory transduction following stimuli on the surface. Exogenous to surface elicit dentinal sensitivity through movement of fluids tubules, resulting cellular deformation. Recently, Piezo1 channels have been implicated mechanosensitive processes, as well Ca 2+ signals odontoblasts. However, human odontoblasts, responses induced by mechanical stimulation, channel expression, its pharmacological properties remain unclear. In present...
According to the "hydrodynamic theory," dentinal pain or sensitivity is caused by fluid movement following application of various stimuli dentin surface. Recent convergent evidence in Vitro has shown that plasma membrane deformation, mimicking movement, activates mechanosensitive transient receptor potential (TRP)/Piezo channels odontoblasts, with Ca2+ signal eliciting release ATP from pannexin-1 (PANX-1). The released P2X3 receptor, which generates and propagates action potentials...
Abstract The craniofacial bones provide structural support for the skull and accommodate vulnerable brain tissue with a protective cavity. bone undergoes constant turnover, which relies on skeletal stem cells (SSCs) and/or mesenchymal (MSCs) their niches. SSCs/MSCs perivascular niche within marrow are well characterized in long bones. As cranial bones, besides marrow, suture mesenchyme has been identified as unique of However, comprehensive study two different cell niches at single‐cell...
Biological aging profoundly impairs the homeostasis of skeletal system. Cellular senescence, a hallmark biological aging, plays an instrumental role in bone disease. The underlying mechanisms cellular triggered by both intracellular and extracellular stimuli, are multifaceted yet to be uncovered. Recent research indicates that acute senescence often serves beneficial roles, such as contributing growth, development, tissue regeneration. By contrast, chronic primarily driven accumulation...
G protein-coupled receptors in trigeminal ganglion (TG) neurons are often associated with sensory mechanisms, including nociception. We have previously reported the expression of P2Y12 receptors, which Gi TG cells. Activating decreased intracellular free Ca2+ concentration ([Ca2+]i). This indicated that adenosine 3',5'-cyclic monophosphate (cAMP) levels can mediate signaling Here, we report more extensive-expression patterns Gs primary cultured isolated from 7-day-old newborn Wistar rats and...
An inflammatory response following dental pulp injury and/or infection often leads to neurogenic inflammation via the axon reflex. However, detailed mechanism underlying occurrence of reflex in remains unclear. We sought examine intracellular cyclic adenosine monophosphate (cAMP) signaling pathway odontoblasts activation Gs protein-coupled receptors and intercellular trigeminal ganglion (TG) neuron–odontoblast communication direct mechanical stimulation TG neurons. Odontoblasts express...
Introduction: Space is a unique environment characterized by isolation from community life and exposure to circadian misalignment, microgravity, space radiation. These multiple differences those experienced on the earth may cause systemic local tissue stress. Autonomic nerves, including sympathetic parasympathetic regulate functions in organs. Saliva secreted salivary gland, which regulated autonomic plays several important roles oral cavity digestive processes. The balance of nervous system...
Introduction Oxidative stress, an imbalance between reactive oxygen species (ROS) production and antioxidant defenses, plays important role in various dental diseases. Local anesthetics are frequently used dentistry. The potential activity of local can contribute to practice. Therefore, this study aimed investigate the ROS-scavenging activities three commonly anesthetics, lidocaine, prilocaine, articaine, focusing on their effects hydroxyl radicals (HO•) superoxide anions (O2•−). Materials...
Abstract The craniofacial bone, crucial for protecting brain tissue and supporting facial structure, undergoes continuous remodeling through mesenchymal (MSCs) or skeletal stem cells in their niches. Gli1 is an ideal marker labeling MSCs osteoprogenitors this region, Gli1-lineage are identified as pivotal bone growth, development, repair, regeneration. Despite its significance, the distribution of across dental, oral, (DOC) regions remains to be systematically explored. Utilizing...
Regeneration of orofacial bone defects caused by inflammation-related diseases or trauma remains an unmet challenge. Parathyroid hormone 1 receptor (PTH1R) signaling is a key mediator remodeling whereas the regulatory mechanisms PTH1R in oral under homeostatic inflammatory conditions have not been demonstrated direct genetic evidence. Here, we observed that deletion Gli1+ progenitors led to increased osteogenesis and osteoclastogenesis. Single-cell bulk RNA-Seq analysis revealed suppressed...