A5019067908- Glioma Diagnosis and Treatment
- Chromatin Remodeling and Cancer
- Neuroblastoma Research and Treatments
- Cancer Mechanisms and Therapy
- Fibroblast Growth Factor Research
- Kruppel-like factors research
- interferon and immune responses
- Cell Adhesion Molecules Research
- Connective tissue disorders research
- Brain Metastases and Treatment
- Cellular Mechanics and Interactions
- Caveolin-1 and cellular processes
- Protein Degradation and Inhibitors
- Microtubule and mitosis dynamics
- Ferroptosis and cancer prognosis
- Cancer, Hypoxia, and Metabolism
- Protease and Inhibitor Mechanisms
- Cancer-related gene regulation
- RNA modifications and cancer
- Mechanisms of cancer metastasis
- MicroRNA in disease regulation
- Proteoglycans and glycosaminoglycans research
- Ocular Oncology and Treatments
- Glycosylation and Glycoproteins Research
- Renal and related cancers
Children's Hospital of Los Angeles
2014-2023
University of Southern California
2014-2023
Children's Center
2011-2022
University of Central Florida
2016
Florida College
2016
Tulane University
2016
Massachusetts Institute of Technology
2016
Nationwide Children's Hospital
2015-2016
The Ohio State University
2016
Children's Hospital at Westmead
2014
Medulloblastoma in children can be categorized into at least four molecular subgroups, offering the potential for targeted therapeutic approaches to reduce treatment-related morbidities. Little is known about role of tumor microenvironment medulloblastoma or its contribution these subgroups. Tumor has been shown an important source targets both adult and pediatric neoplasms. In this study, we investigated hypothesis that expression genes related tumor-associated macrophages (TAM) correlates...
Background While major advances have been made in improving the quality of life and survival children with most forms medulloblastoma (MB), those MYC-driven tumors (Grp3-MB) still suffer significant morbidity mortality. There is an urgent need to explore multimodal therapeutic regimens which are effective safe for children. Large-scale studies revealed abnormal cancer epigenomes caused by mutations structural alterations chromatin modifiers, aberrant DNA methylation, histone modification...
Abstract Although tumors express potentially immunogenic tumor‐associated antigens (TAAs), cancer vaccines often fail because of inadequate antigen delivery and/or insufficient activation innate immunity. Engineering nonpathogenic bacterial vectors to deliver TAAs choice may provide an efficient way presenting in form. In this study, we used genes Salmonella pathogenicity island 2 (SPI2) construct a novel vaccine which TAA, survivin, was fused SseF effector protein and placed under control...
Abstract Background Choroid plexus tumors (CPT) are rare, and predominate in early childhood. An association with the Li–Fraumeni syndrome (LFS) has been reported, but biological clinical implications of this remain poorly defined. We have investigated features overall survival all CPT patients treated at Children's Hospital Los Angeles (CHLA) over a 20‐year period, particular attention to LFS. Methods A retrospective evaluation course therapy outcome was undertaken on 42 diagnosed for CHLA...
Glioblastoma (GBM) is a highly invasive brain tumor. Perivascular invasion, autovascularization and vascular co-option occur throughout the disease lead to tumor invasion progression. The molecular basis for perivascular i.e., interaction of glioma cells with endothelial not well characterized. Recent studies indicate that have increased expression CXCR4. We investigated in-vivo role CXCR4 in using shRNA-mediated knock down show primary cultures human stem HF2303 mouse GL26-Cit exhibit...
// Melanie F. Weingart 1 , Jacquelyn J. Roth 7 Marianne Hutt-Cabezas Tracy M. Busse 8 Harpreet Kaur Antoinette Price Rachael Maynard Jeffrey Rubens Isabella Taylor Xing-gang Mao Jingying Xu 4 Yasumichi Kuwahara 3 Sariah Allen 6 Anat Erdreich-Epstein 4,5 Bernard E. Weissman Brent A. Orr Charles G. Eberhart Jaclyn Biegel 7,8,9 and Eric H. Raabe 1,2 Division of Neuropathology Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD, USA 2 Pediatric Oncology, Lineberger...
Medulloblastomas in children can be categorized into 4 molecular subgroups with differing clinical characteristics, such that subgroup determination aids prognostication and risk-adaptive treatment strategies. Magnetic resonance spectroscopy (MRS) is a widely available, noninvasive tool used to determine the metabolic characteristics of tumors provide diagnostic information without need for tumor tissue. In this study, we investigated hypothesis metabolite concentrations measured by MRS...
Pediatric brain tumors as a group, including medulloblastomas, gliomas, and atypical teratoid rhabdoid (ATRT) are the most common solid in children leading cause of death from childhood cancer. Brain tumor-derived cell lines critical for studying biology pediatric can be useful initial screening new therapies. Use appropriate tumor experiments is important, results may differ depending on properties, thus affect conclusions applicability model. Despite reports literature over 60 lines,...
Extraocular retinoblastoma is associated with a very poor outcome. At Children's Hospital Los Angeles, 10 of 207 patients had extraocular disease. Four no histopathologic risk factors developed All direct extension into the central nervous system or distant metastatic disease died. One three trilateral and one patient regional recurrence are alive after autologous bone marrow transplant. Patients who achieve remission may benefit from consolidation their therapy
Stress stimuli can mediate apoptosis by generation of the lipid second messenger, ceramide. Herein we investigate molecular mechanism ceramide signaling in endothelial induced fenretinide (<i>N</i>-(4-hydroxyphenyl)retinamide (4-HPR)). 4-HPR, a synthetic derivative retinoic acid that induces tumor cell lines, has been shown to have antiangiogenic effects, but these is largely unknown. We report 4-HPR was cytotoxic cells (50% cytotoxicity at 2.4 μm, 90% 5.36 μm) and caspase-dependent...
Abstract The bone is the third most common site of cancer metastasis. To invade bone, tumor cells produce osteoclast-activating factors that increase resorption by osteoclasts. Here we report human neuroblastoma form osteolytic lesions in vivo do not but rather stimulate osteoclast activity presence marrow mesenchymal stem cells. This alternative pathway activation involves a nonadhesive interaction between and Stimulated express markedly increased levels interleukin-6, which then...
Nuclear factor IA (NFIA), a transcription and essential regulator in embryonic glial development, is highly expressed human glioblastoma (GBM) compared with normal brain, but its contribution to GBM cancer pathogenesis unknown. Here we demonstrate novel role for NFIA promoting growth migration of establish the molecular mechanisms mediating these functions. To determine glioma, examined effects growth, proliferation, apoptosis, migration. We used gain-of-function (overexpression)...
Background Recurrence occurs in almost 50% of patients with intracranial ependymoma, and their outcome following recurrence is poor. Methods We retrospectively reviewed the medical records 22 ependymoma subsequent relapse(s) (59 recurrences) treated at Children's Hospital Los Angeles or New York University between January 1997 December 2012. Results Median duration follow‐up was 52 months (7–171 months). age initial diagnosis 4 years (0.3–19 years) 8 younger than 3 presentation. Eleven had...
High-grade gliomas are characterized by exuberant vascularization, diffuse invasion, and significant chemoresistance, resulting in a recurrent phenotype that makes them impossible to eradicate the long term. Targeting protumoral signals glioma microenvironment could have impact against tumor cells supporting niche facilitates their growth. Fibulin-3 is protein secreted cells, but absent normal brain, promotes invasion survival. We show here fibulin-3 paracrine activator of Notch signaling...
Nuclear factor IA (NFIA) is a transcription that specifies glial cell identity and promotes astrocyte differentiation during embryonic development. Its expression function in gliomas are not known. Here, we examined NFIA protein its association with clinical outcome pediatric malignant astrocytomas. We analyzed of by immunohistochemistry 88 existing glioma specimens from Childrens Hospital Los Angeles the University Southern California. Association between progression-free survival (PFS) was...
Abstract BACKGROUND: The role of myeloablative chemotherapy in children with recurrent medulloblastoma and supratentorial primitive neuroectodermal tumors (MB/ST‐PNET) is controversial, particular patients who develop disease after craniospinal radiotherapy. METHODS: In this retrospective analysis, the authors investigated outcome MB/ST‐PNET were referred for autologous hematopoietic progenitor cell rescue at Childrens Hospital Los Angeles. RESULTS: Thirty‐three chemotherapy: Fourteen those...
Abstract Heparanase is the only mammalian endoglycosidase which has been widely implicated in cancer because of its capability to degrade heparan sulfate chains proteoglycans (HSPG). Specifically, cell surface HSPG syndecan-1 and -4 (SDC1 SDC4) are modulators growth factor action, SDC4 adhesion as a key member focal complexes. We hypothesized that extracellular heparanase modulates brain metastatic breast (BMBC) invasiveness by affecting cytoskeletal dynamics, carboxy-terminal–associated...
Abstract The glio-vascular unit (G-unit) plays a prominent role in maintaining homeostasis of the blood-brain barrier (BBB) and disturbances cells forming this may seriously dysregulate BBB. direct indirect effects cytokines on cellular components BBB are not yet unclear. present study compares cytokine-treated astrocytes brain endothelial barrier. 3-dimensional transwell co-cultures endothelium related-barrier with were used to investigate gliovascular responses during pathological...