// Ran Liu 1, * , Huiting Wei 2, Peng Gao 3 Hu Yu 4 Ke Wang Zheng Fu 2 Baohui Ju 5 Meng Zhao Shangwen Dong 6 Zhijun Li Yifeng He 7 Yuting Huang and Zhi Yao 1 Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science Technology, University, Tianjin, 300072, China Department Immunology, Cellular Molecular Educational Ministry China, Basic Medical Sciences, 300071, University the District Columbia, Washington D.C., 20008, United States Cancer...

Non-small-cell lung cancer (NSCLC) constitutes the leading cause of death in humans. Previous studies revealed essential role protein arginine methyltransferase 7 (PRMT7) promoting metastasis breast cancer. However, its function and potential mechanism NSCLC remain unclear.The gene expression PRMT7 between tissues normal was studied with online database (http://medicalgenome.kribb.re.kr/GENT/). cell lines specific overexpression were constructed lentivirus transduction. Matrigel invasion...

The present study aimed to investigate the expression and role of SET MYND domain-containing protein 3 (SMYD3) in esophageal squamous cell carcinoma; observe proliferation carcinoma after suppression SMYD3 expression; explore effect downregulation on retinoblastoma protein-interacting zinc finger gene 1 (RIZ1). Tissues from 11 patients, including cancer normal tissues, were obtained by surgery immunohistochemistry. Esophageal TE13 cells transfected with four different SMYD3-shRNA plasmids,...

Lipopolysaccharide (LPS) is a major component of the outer wall gram negative bacteria. In high doses LPS contributes to inflammation in sepsis and low grade characteristic metabolic syndrome. We wanted assess role beta2-glycoprotein I (β2GPI) highly conserved plasma protein its different biochemical forms mouse model systemic inflammation. Normal β2GPI deficient mice were administered through their veins assessed for range markers blood liver. Different measured normal given either saline...

Purpose Emerging evidence indicates that circulating microRNAs (miRs) might act as noninvasive biomarkers for cancer diagnosis and prognosis. We examined the expression pattern clinical significance of plasma miR-9 in patients with esophageal squamous cell carcinoma (ESCC). Methods Venous blood samples (6 mL) were collected from 131 ESCC healthy controls, concentration was detected by reverse transcription polymerase chain reaction. The association clinicopathologic factors survival...

AIM:To investigate the promoter region methylation status of retinoblastoma protein-interacting zinc finger gene 1 (RIZ1) in human esophageal squamous cell carcinoma (ESCC) lines and tissues veri�y relationship between o� RIZ1 oncogenesis, tumor progression metastasis etc ESCC. METHODS:Methylation-specific polymerase chain reaction (MSP) was used to 6 ESCC lines.One line where detected selected �or next study, treated with 5-aza-CdR.Real-time its influence on transcription RIZ1.Experiments...

The new TomoDirect (TD) modality offers a nonrotational option with discrete beam angles. We aim to compare dosimetric parameters of TD, helical tomotherapy (HT), volumetric-modulated arc therapy (VMAT), and fixed-field intensity-modulated radiotherapy (ff-IMRT) for upper thoracic esophageal carcinoma (EC).Twenty patients cT2-4N0-1M0 squamous cell (ESCC) were enrolled. Four plans generated using the same dose objectives each patient: HT, VMAT single arc, ff-IMRT 5 fields (5F). prescribed...

Identification of biomarkers for predicting radiosensitivity would be useful administering individualized radiotherapy (RT) to patients with esophageal cancer. The aim the present study was evaluate association between cyclooxygenase-2 (COX-2), X-ray repair cross complementing group 1 (XRCC1), ras domain family (RASSF1) protein expression, clinicopathological characteristics, and survival rate in 76 squamous cell carcinoma (ESCC) who were treated RT. Positive expression COX-2, XRCC1 RASSF1...

The tumor suppressor protein retinoblastoma protein‑interacting zinc finger gene 1 (RIZ1) is downregulated in several types of cancer, including esophageal squamous cell carcinoma (ESCC). present study used two vitro methods to re‑express RIZ1 the human ESCC TE13 line order induce apoptosis. was re‑expressed cells by reintroducing through transfection or removal transcriptional repression treatment with a DNA methyltransferase (DNMT) inhibitor. To reintroduce gene, open reading frame...

// Yang Lu 1, * , Fanjie Meng 2, 3 Lan Li 4 Donghao Wang 1 Yuantao Cui 2 Shangwen Dong and Wanhua Department of Intensive Care Unit, Tianjin Medical University Cancer Institute Hospital, National Clinical Research Center for Cancer, Key Laboratory Prevention Therapy, Tianjin, Tianjin's 300060, China Cardiothoracic Surgery, General 300052, Anesthesia, Pharmacology Traditional Chinese Medicine Formulae, Ministry Education, Medicine, 300193, These authors have contributed equally to this work...

Malignant fibrous histiocytoma (MFH) is an aggressive soft tissue sarcoma known to occur in various organs. Primary MFH arising the lung quite rare. Herein we report a case of 61-year-old male with primary pulmonary and explore underlying molecular mechanisms by next-generation sequencing (NGS). Five gene mutations TSC2, ARID1B, CDK8, KDM5C CASP8 were detected, mTOR inhibitor might be effective treatment for this patient. In addition, reviewed scientific literature approximately 23 reports...

The immune responses of males and females to bacterial infections display differences. mechanisms that underlie this sexual dimorphism are multifactorial. Lipopolysaccharide (LPS) contributes the pathogenesis endotoxaemia. We have previously demonstrated plasma protein beta-2 glycoprotein-1 (β2GPI) reduces LPS-induced inflammation in male mice. In present study using a more robust infection model septicaemia role β2GPI is examined both female wild type (WT) deficient (β2GPI-/-) mice...

This study aimed to select piperacillin/tazobactam (TZP) infusion mode guided by Sequential Organ Failure Assessment (SOFA) score in cancer patients with hospital-acquired pneumonia (HAP) postoperation.A total of 120 postoperative HAP were divided into two groups: improved administration group (L group) and conventional treatment (Con group). The Con received traditional TZP the L it as prolonged infusion. Blood drug concentration was detected at different time points. Based on SOFA cut-off...

AIM:To investigate the effect of retinoblastoma protein-interacting zinc finger gene 1 (RIZ1 ) upregulation in expression profile and oncogenicity human esophageal squamous cell carcinoma (ESCC) line TE13.METHODS: TE13 cells were transfected with pcD-NA3.1(+)/RIZ1 pcDNA3.1(+).Changes screened microarray results confirmed by reverse transcriptionpolymerase chain reaction (RT-PCR).Nude mice inoculated to establish ESCC xenografts.After two weeks, randomly divided into three groups.Tumors...

Abstract Retinoblastoma protein-interacting zinc finger gene RIZ encodes two different protein products, RIZ1 and RIZ2. Observations suggest that is a tumor suppressor, while RIZ2 acts as negative regulator of may play positive role in oncogenesis. The imbalance amount be involved cancer development. In this study we detected the expression levels mRNA human esophageal squamous cell carcinoma (ESCC) tissue specimens, reexpressed ESCC line EC109 which level was not detected, examined changes...

To study the expression of Syk (spleen tyrosine kinase) gene in human ESCC (esophageal squamous cell carcinoma).We used immunohistochemical staining to detect protein esophageal carcinoma tissues and RT-PCR (semi-quantitative reverse transcription PCR), Real-time PCR (Real-time quantitative PCR) western blotting technique mRNA specimens from 4 lines.Immunohistochemistry analyses showed that cancer was expressed inferior adjacent normal one, p < 0.05. Western low detected lines compared with...

In humans, males compared to females have increased visceral adipose tissue which contributes their risk of early death. Mice display analogous sexual diamorphism whereby are protected from weight gain when fed a high fat diet males. A role has recently been reported for β2-glycoprotein I, an abundant plasma protein, in healthy leanness humans. this study we investigated the I metabolism male and female mice normal chow or diet. We made number novel insights into factors contributing...

PR domain is responsible for the tumor suppressing activity of RIZ1. The study aimed to construct human eukaryotic expression vectors, transfect esophageal cancer cells (TE13), and evaluate anticancer on TE13 cells.First, mRNA was extracted from tissue by RT-PCR, then reverse-transcribed cDNA. After amplifying DNA template, linked T vector. Second, after extraction, cut using enzyme pcDNA3.1(+). Then, plasmid transfered Trans1-T1 phage resistant competent cells, following extracting...

The present study examined the role of PRDI-BF1-RIZ (PR) domain tumor suppressor retinoblastoma protein-interacting zinc finger gene 1 (RIZ1) as an anticancer and its ability to induce apoptosis in esophageal squamous cell carcinoma (ESCC) cells. TE13 ESCC line was transfected with pcDNA3.1(+) eukaryotic expression vectors bearing open reading frames either human RIZ1 or PR domain, mRNA protein levels were then detected using quantitative reverse transcription polymerase chain reaction...

Objective To observe the effects of retinoblastoma protein-interacting zinc finger gene 1 (RIZ1) on expression profile esophageal squamous cell carcinoma line TE13.Methods The TE13 cells were cultured and divided into 10 portions,subcultured transfected respectively by pcDNA3.1 (+) (+)/RIZ1 using lipofectamin transfection reagent.After successful transfection,Agilent whole human genome oligo microarray (4 * 44K) was used to screen RIZ1 (foldchange ≥2 or ≤0.5).The test results confirmed...