- Amoebic Infections and Treatments
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Parasitic Infections and Diagnostics
- Plant Virus Research Studies
- Antibiotic Resistance in Bacteria
- Traditional Chinese Medicine Analysis
- Chemical synthesis and alkaloids
- Food Allergy and Anaphylaxis Research
- Plant biochemistry and biosynthesis
- Essential Oils and Antimicrobial Activity
- Bacteriophages and microbial interactions
- Plant Pathogenic Bacteria Studies
- Animal Virus Infections Studies
- Allergic Rhinitis and Sensitization
- Plant pathogens and resistance mechanisms
- Enzyme Production and Characterization
- Traditional and Medicinal Uses of Annonaceae
- Legume Nitrogen Fixing Symbiosis
- Plant Parasitism and Resistance
- Veterinary medicine and infectious diseases
- Natural product bioactivities and synthesis
- Transgenic Plants and Applications
- Sesquiterpenes and Asteraceae Studies
- Phytochemistry and Biological Activities
Instituto Politécnico Nacional
2011-2024
Institute for Scientific and Technological Research
2014-2021
Consejo Nacional de Humanidades, Ciencias y Tecnologías
2020-2021
Center for Research and Advanced Studies of the National Polytechnic Institute
2010-2014
Instituto Tecnológico Superior de Irapuato
2014
Comprehensive in silico analysis aimed to identify the genes involved biosafety and probiotic features of Limosilactobacillus fermentum J23 was performed. The strain had a single circular chromosome 2,004,882 base pairs (bp), with Guanine + Cytosine (G C) content 51.74%. contained 2,001 predicted as protein-coding sequences (CDS), 86 pseudogenes, 56 tRNAs, five rRNAs, three ncRNAs. demonstrated absence risk-related corresponding transferable/acquirable antibiotic resistance, virulence...
A novel bipartite begomovirus, Blechum interveinal chlorosis virus (BleICV), was characterized at the genome level. Comparative analyses revealed that BleICV coat protein (CP) gene promoter is highly divergent from equivalent region of other begomoviruses (BGVs), with single exception Tomato chino La Paz (ToChLPV) which it shares a 23-bp phylogenetic footprint exhibiting dyad symmetry. Systematic examination homologous CP segment 132 New World BGVs existence quasi-palindromic DNA displaying...
Eleven neo-clerodane diterpenoids (1–11) including the new analogues 1, 2, and 10, 3′,5,6,7-tetrahydroxy-4′-methoxyflavone (12) were isolated from aerial parts of Salvia polystachya. Polystachyne G (1) 15-epi-polystachyne (2) as an epimeric mixture, containing a 5-hydroxyfuran-2(5H)-one unit in side chain at C-12 framework. H (10) contains 1(10),2-diene moiety tertiary C-4 hydroxy group. The structures these compounds established by analysis their NMR spectroscopic MS spectrometric data....
The begomoviruses (BGVs) are plant pathogens that evolved in the Old World during Cretaceous and arrived to New (NW) Cenozoic era. A subgroup of NW BGVs, "Squash leaf curl virus (SLCV) lineage" (S-Lin), includes viruses with unique characteristics. To get clues on evolutionary origin this lineage, a search for divergent members was undertaken. Four novel BGVs were characterized, including one is basal group. Comparative analyses led discover ~670 bp genome module nearly exclusive...
Entamoeba histolytica encodes four family B2 DNA polymerases that vary in amino acid length from 813 to 1279. These contain a N-terminal domain with no homology other proteins and C-terminal high identity archetypical polymerases. A phylogenetic analysis indicates these are grouped transposable elements dubbed Polintons or Mavericks. In this work, we report the cloning biochemical characterization of smallest polymerase E. histolytica. To facilitate its subcloned 660 acids region comprises...
Abstract Background Pseudomonas syringae pv. phaseolicola, the causal agent of halo blight disease in beans, produces a toxin known as phaseolotoxin, whose synthesis participate group genes organized within genome region "Pht cluster". This region, which is thought to have been acquired by horizontal gene transfer, includes 5 transcriptional units, two monocistronic ( argK, phtL ) and three polycistronic phtA, phtD, phtM ), expression temperature dependent. So far, regulatory mechanisms...
Background Eukaryotic family A DNA polymerases are involved in mitochondrial replication or translesion synthesis. Here, we present evidence that the sole polymerase from parasite protozoan E. histolytica (EhDNApolA) localizes to nucleus and its biochemical properties indicate this may be Methodology Results EhDNApolA is histolytica. An silico analysis places genus Entamoeba a separate branch of phylogenetic tree. Biochemical studies purified recombinant demonstrated active primer...
The protozoan parasite Entamoeba histolytica is exposed to reactive oxygen and nitric oxide species that have the potential damage its genome. E. harbors enzymes involved in DNA repair pathways like Base Nucleotide Excision Repair. majority of repairs converge their final step which a ligase seals nicks. In contrast other eukaryotes, genome encodes only one (EhDNAligI), suggesting this both replication repair. Therefore, aim work was characterize EhDNAligI, ligation fidelity ability ligate...