MicroRNA-21 was upexpressed in gastric cancer (GC) indicating that it is a potential diagnostic biomarker for GC. In this study, 50 GC patients and healthy controls were recruited. miR-21 levels serum peripheral blood mononuclear cells (PBMCs) quantified using quantitative real-time PCR. CA199, CEA measured electrochemiluminescence assay. The sensitivity specificity of circulating miR-21, CA199 diagnosis, the correlation to clinicopathological features, value different stages determined....

Serum and glucocorticoid-inducible kinase (SGK) 1can be triggered in several malignancies. Most research on SGK1has focused its role cancer cells, we sought to investigate potential upstream non-coding RNA nominated as Lnc-SGK1, their expression diagnostic value T cells human gastric (GC). Excessive of Lnc-SGK1 SGK1 were observed cell either within the tumor or peripheral furthermore associated with Helicobacter pylori infection high-salt diet (HSD). Within (Hp) dietcan up-regulated turn...

Objectives The role of the gut-liver axis in liver cirrhosis is becoming increasingly recognized. We investigated fecal microbiome patients with and its potential function as a predictive biomarker hepatic encephalopathy. Methods Patients were divided into either high plasma ammonia (HPA) group or low (LPA) according to upper limit normal concentration. 16S rRNA sequencing samples was performed study how microbiota affects clinical symptoms cirrhosis. Stroop test used assess ability brain...

To understand the prevalence of functional constipation in pregnant women and to analyze impact its risk factors.We searched hospital databases for who were 37-41 weeks (1698 cases) from July 2012 January 2014 four hospitals Shanghai. We reviewed factors including general data, living eating habits, psychological history, past history defecation 6 months before pregnancy after pregnancy. Data analyzed using SPSS software.Pregnant more than 35 years old, with a pre-pregnancy body mass index...

Exosomes, membranous nanovesicles, naturally carry bio-macromolecules or miRNA and play impoetant roles in tumor pathogenesis. Here, we showed that macrophages cell-derived exosomes can function as vehicles to deliver exogenous miR-21 inhibitor into BGC-823 gastric cancer cells. Exosomes loaded with miR-21inhibitor significantly increased levels BGC-823, but exerted an opposite effect. transfected had less cellular toxicity host cells compared conventional transfection methods. The promoted...

Abstract Abnormal tumor microenvironment and the epithelial‐mesenchymal transition (EMT) are important features of metastasis. However, it remains unknown how signals can form complicated networks to regulate sustainability EMT process. The aim our study is explore possible interaction between tumor‐associated macrophages cells in process mediated by microRNA (miR)‐362‐3p. In this study, we found that releasing TGF‐β, M2 mediate binding Smad2/3 miR‐362‐3p promoter, leading overexpression...

YAP1 is a core protein of the Hippo signaling pathway and associated with malignancy immunosuppression. In present study, we discovered novel lncRNA, RP11-323N12.5, tumor promotion immunosuppression activities through enhancing transcription YAP1. RP11-323N12.5 was identified using GEPIA. Its expression levels their relationship clinical features were investigated samples. The regulation by in both GC T cells, roles explored vitro vivo. most up-regulated lncRNA human GC, based on data from...

Although mycobacterial proteins in exosomes from peripheral serum of patients with tuberculosis (TB) have been identified, other exact compositions remain unknown. In the present study, a comprehensive proteomics analysis derived active TB (ATB) was performed. Exosomes ATB were characterized using nanoparticle tracking (NTA), transmission electron microscopy (TEM), and western blotting analysis. Then identified protein components quantified by label-free determined via bioinformatics A total...

Context: The sensitivity and specificity of blood microRNA-421 (miR-421) as a gastric cancer (GC) biomarker has not been determined.Objective: To investigate the diagnostic value miR-421 GC biomarker.Materials methods: in serum peripheral mononuclear cells (PBMCs) 90 patients controls was detected by quantitative real-time PCR. correlation to clinicopathological features well comparing carcinoembryonic antigen (CEA) 125 (CA-125) were analyzed.Results: increased significantly than controls....

MicroRNAs might act as oncogenes or tumor suppressors in cancer. Recent studies have shown that miR-421 is up-regulated human gastric Here, we found was over-expressed cancer tissues and cell lines. Bioinformatics analysis predicted the caspase-3 gene a target of miR-421. Caspase-3 negatively regulated by at post-transcriptional level. Bax Bcl-2 were also Moreover, necrosis factor receptor-I -II, death receptors apoptosis pathway, The over-expression promoted growth inhibited BGC-823 line....

Targeted nanocarriers may offer a new approach to improve the efficacy and tolerability of cisplatin, which are commonly used treat cancers as first line chemotherapy for most types cancer. In present study, we have developed EGFR-targeted albumin-cisplatin nanoparticles tumor targeted delivery cisplatin. The cisplatin NPs were conjugated with EGFR aptamer, binded Hela cells specifically, then taken up by through receptor mediated endocytosis. aptamers accumulate in interact on surface...

Previous studies have revealed that exosomes influence tumour metastasis, diagnosis and treatment. In addition, exosomal microRNAs (miRNAs/miRs) are closely associated with the metastatic microenvironment; however, regulatory role of miRNAs from prostate cancer cells on bone metastasis remains poorly understood. present study, a series experiments were performed to determine whether miR-375 LNCaP promote osteoblast activity. Exosomes isolated purified by ultracentrifugation, total RNA miRNA...

The aim of this study is to investigate the effects polyphenol extract from Phyllanthus emblica (PEEP) on cervical cancer cells and explore underlying mechanism.MTT assay was used measure inhibition proliferation (HeLa) after treatment with PEEP at concentrations 0, 50, 100, 150, 200 mg/ml for 48 hours. HeLa were treated (150 mg/ml) hours in following analysis. Karyomorphism assessed by immunofluorescence using DAPI staining, cell apoptosis cycle flow cytometry. Three apoptotic marker...