A5036997116- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Hepatitis C virus research
- Digital Innovation in Industries
- Hepatitis B Virus Studies
- Public Administration and Political Analysis
- HIV-related health complications and treatments
- Biochemical and Molecular Research
- Herpesvirus Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Pneumocystis jirovecii pneumonia detection and treatment
- Linguistic research and analysis
- German Literature and Culture Studies
- Health and Medical Studies
- Respiratory viral infections research
- Genomics and Phylogenetic Studies
- Education Methods and Technologies
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Parvovirus B19 Infection Studies
- Viral-associated cancers and disorders
- Families in Therapy and Culture
- Blood groups and transfusion
- Public Relations and Crisis Communication
Goethe University Frankfurt
2007-2020
University Hospital Frankfurt
2004-2020
Center for HIV and Hepatogastroenterology
2020
Bard Access Systems (United States)
2013
Institute of Virology of the Slovak Academy of Sciences
2003-2005
Bernhard Nocht Institute for Tropical Medicine
2003
Zentrum für Seelische Gesundheit
2002
BackgroundAbstractThe severe acute respiratory syndrome (SARS) has recently been identified as a new clinical entity. SARS is thought to be caused by an unknown infectious agent.MethodsClinical specimens from patients with were searched for viruses the use of cell cultures and molecular techniques.ResultsA novel coronavirus was in SARS. The virus isolated culture, sequence 300 nucleotides length obtained polymerase-chain-reaction (PCR)–based random-amplification procedure. Genetic...
ABSTRACT To investigate the occurrence of multinucleoside analog resistance during therapy failure, we surveyed drug susceptibilities and genotypes nearly 900 human immunodeficiency virus type 1 (HIV-1) samples. For 302 these, 50% inhibitory concentrations at least four approved nucleoside analogs had fourfold-or-greater increases. Genotypic analysis reverse transcriptase (RT)-coding regions from these samples revealed complex mutational patterns, including previously recognized codon 151...
We describe a new human immunodeficiency virus type 1 (HIV-1) mutational pattern associated with phenotypic resistance to lamivudine (3TC) in the absence of characteristic replacement methionine by valine at position 184 (M184V) reverse transcriptase. Combined genotypic and analyses clinical isolates revealed presence moderate levels (between 4- 50-fold) 3TC subset that did not harbor M184V mutation. Mutational cluster analysis comparison data significant correlation between an increased...
Current HIV-1 viral-load assays are too expensive for resource-limited settings. In some countries, monitoring of antiretroviral therapy is now more than treatment itself. addition, commercial have shown shortcomings in quantifying rare genotypes.
Noroviruses (NV) are transmitted by fecally contaminated food, vomit, and person-to-person contact. They one of the main causes non-bacterial acute gastroenteritis in nursing, old people children’s homes. NV outbreaks characterized a short incubation period (12–48 h), nausea, vomiting diarrhea, high secondary attack rates. The illness is generally mild self-limiting. aim diagnostic procedures viral to avoid nosocomial infections on hand unnecessary antibiotic treatment other. Diagnostic for...
<i>Background:</i> Genotypic drug resistance testing provides essential information for guiding treatment in HIV-infected patients. It may either be used identifying patients with transmitted or to clarify reasons failure and check remaining options. While different approaches the interpretation of HIV sequence are already available, no other available rules-based systems specifically have looked into effects combinations drugs. HIV-GRADE (Genotypischer Resistenz Algorithmus...
Objective: To investigate the prevalence and magnitude of M184V-mediated changes in susceptibility to zalcitabine, didanosine, stavudine abacavir (1592U89 succinate) a cohort lamivudine-treated patients. Design methods: A total 255 samples from patients treated with lamivudine zidovudine or without other nucleoside reverse transcriptase inhibitors (NRTI) were analysed for zidovudine, lamivudine, didanosine using recombinant virus assay. Seventy-three originated exposed only. subset 27 was...
HIV-1 genotyping has become a widely accepted tool for monitoring antiretroviral therapy. However, the benefit of is limited by need to interpret mutation pattern in order obtain prediction regarding susceptibility each drug. Several different interpretation systems are currently available, commercially or free charge; some combination with test system used. In this study, we compared results obtained on patient samples, using nine resistance genotype, and identified patterns responsible...
Human immunodeficiency virus type 1 (HIV-1) strains resistant to nonnucleoside reverse transcriptase inhibitors (NNRTIs) may easily be selected for in vitro and vivo under a suboptimal therapy regimen. Although cross-resistance is extensive within this class of compounds, newer NNRTIs were reported retain activity against laboratory containing defined resistance-associated mutations. We have characterized HIV-1 resistance loviride the extent nevirapine, delavirdine, efavirenz, HBY-097,...
Considering human immunodeficiency virus type 2 (HIV-2) phenotypic data and experience from HIV 1 the follow-up of HIV-2–infected patients, a panel European experts voted on rule set for interpretation mutations in HIV-2 protease, reverse transcriptase, integrase an automated tool drug resistance analyses freely available Internet (http://www.hiv-grade.de).
ABSTRACT Zidovudine resistance (ZDV-R) is associated with classic genotypic changes at codons 41, 67, 70, 210, 215, and 219 of the human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) gene as well multinucleoside (MNR) complexes (Q151M MNR complex; 6-bp insertion/A62V complex). In addition, enhanced to ZDV in context mutations plus M184V mutation has been additional positions 208, 211, 214, 333. this study we investigated phenotypic ZDV-R determined by a recombinant assay...
Highly active antiretroviral therapy (HAART) has significantly reduced morbidity and mortality in HIV-infected patients. However, problems such as short-term or long-term toxicity the development of drug resistance could necessitate a change regimen. Whereas various HAART options with low pill burden favourable tolerability profiles are available for naive patients, treatment experienced patients tends to be more complex remains challenge. Treatment class sparing nucleoside-only regimens an...
Introduction HIV-1 has a high replication rate of about 109 viruses produced each day [1,2]. Because the relatively error reverse transcriptase (RT) 1 : 1000 to 10 000 errors per incorporated base [3,4], there is degree variability between different virus isolates at genomic level [5]. However, if two have common source, variation should be dependent on time elapsed since transmission event; transmission, been virtually indistinguishable. This fact shown for env and gag genes [6,7]. For that...
The objective of this study was to identify parameters among saquinavir pharmacokinetics, patients' demographics or comedications, be addressed for improved personalized therapy. presence human immunodeficiency virus type 1 (HIV-1) RNA at therapy week 48 (principal target parameter), CD4 cell count 48, infections and side effects during weeks, indicators liver toxicity lipid abnormalities a 12-h plasma concentration-versus-time profile were assessed in 56 patients receiving...
Human immunodeficiency virus-2 (HIV-2) is endemic in some countries West Africa. Due to the lower prevalence industrialized countries, there limited experience and knowledge on management of individuals living with HIV-2 Europe. Compared HIV-1, are differential characteristics regarding diagnostic procedures, clinical course, and, most importantly, antiretroviral therapy. We integrated published literature (studies reports epidemiology, diagnostics, treatment), as well expert diagnosing...
Genotypic testing for HIV-1 resistance to anti-retroviral drugs has become accepted widely as a routine method guide therapy. However, implementation into high-throughput laboratory diagnosis is difficult due the complexity of assay. A commercially available assay ViroSeq Genotyping System (Applied Biosystems, Weiterstadt, Germany). We modified and substituted RNA extraction module optimize proportion samples amplified successfully follows: 1 ml plasma was concentrated by ultracentrifugation...