A5086395850- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Protist diversity and phylogeny
- Respiratory viral infections research
- Marine and coastal ecosystems
- Aquaculture disease management and microbiota
- Enzyme Production and Characterization
- Methane Hydrates and Related Phenomena
- Plant Disease Resistance and Genetics
- Actinomycetales infections and treatment
- Infective Endocarditis Diagnosis and Management
- Identification and Quantification in Food
- Cassava research and cyanide
- Metabolomics and Mass Spectrometry Studies
- Microbial infections and disease research
- RNA modifications and cancer
- Isotope Analysis in Ecology
- Plant Virus Research Studies
- Enzyme Structure and Function
Shandong University
2016-2024
Shandong University of Science and Technology
2018-2024
Xiamen University
2012-2022
Dalhousie University
2022
University of Maryland, Baltimore
2015
University of Maryland Center for Environmental Science
2015
The Roseobacter clade is abundant and widespread in marine environments plays an important role oceanic biogeochemical cycling. In this present study, a lytic siphophage (labeled vB_DshS-R5C) infecting the strain type of Dinoroseobacter shibae named DFL12T, which part clade, was isolated from oligotrophic South China Sea. Phage R5C showed narrow host range, short latent period low burst size. genome length phage 77, 874 bp with G+C content 61.5%. Genomic comparisons detected no matches...
Summary Cyanophages are thought to affect the community structure, population dynamics, metabolic activity and evolution of picocyanobacteria impact biogeochemical cycling in aquatic ecosystems. Here, we report an estuarine Synechococcus phage, S‐CBWM1, which represents a novel viral lineage exhibits interesting genetic features related phage–host interactions evolution. S‐CBWM1 encapsidates four virion‐associated proteins cellular regulation. Several auxiliary genes multidrug efflux, cell...
Viruses are infectious and abundant in the marine environment. Viral lysis of host cells releases organic matter nutrients that affect surrounding microbial community. Synechococcus important primary producers ocean they subject to frequent viral infection. In laboratory, cultures often associated with bacteria such a co-existence relationship appears be growth stability . However, we know little about how affects co-existing culture. This study investigated influence infection on...
In recent metagenomic studies, single-stranded DNA (ssDNA) viruses that infect bacteria have been shown to be diverse and prevalent in the ocean; however, there are few isolates of marine ssDNA phages. Here, we report on a cultivated phage (vB_Cib_ssDNA_P1) infects Citromicrobium bathyomarinum RCC1878 (family Sphingomonadaceae), other members genus. This is first reported alphaproteobacteria, represents newly recognized lineage Microviridae infecting Sphingomonadaceae, Amoyvirinae. The ∼26...
Picocyanobacteria are major primary producers in the ocean, especially tropical or subtropical oceans during warm seasons. Many "warm" picocyanobacterial species have been isolated and characterized. However, picocyanobacteria cold environments seasons much less studied. In general, little is known about taxonomy ecophysiology of living winter. this study, 17 strains were from Chesapeake Bay, a temperate estuarine ecosystem, winter months. These isolates belong to five distinct phylogenetic...
A Gram-positive, motile, short-rod-shaped bacterium, designated strain JLT1488(T), was isolated from the South China Sea and investigated in a taxonomic study using polyphasic approach. The peptidoglycan type determined for JLT1488(T) A4α with lysine as diagnostic cell-wall diamino acid an interpeptide bridge of L-Lys-L-Glu. polar lipids consisted diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, unknown glycolipid phospholipid. only...
Cyanophages, viruses that infect cyanobacteria, are abundant and widely distributed in aquatic ecosystems, playing important roles regulating the abundance, activity, diversity, evolution of cyanobacteria. A T4-like cyanophage, S-SCSM1, infecting Synechococcus Prochlorococcus strains different ecotypes, was isolated from South China Sea this study. For first time, a mannose-6-phosphate isomerase (MPI) gene identified cultured cyanophage. At least 11 phylogenetic clusters cyanophage MPIs were...
A prophage vB_CibM-P1 was induced by mitomycin C from the epipelagic strain Citromicrobium bathyomarinum JL354, a member of alpha-IV subcluster marine aerobic anoxygenic phototrophic bacteria (AAPB). The bacteriophage had Myoviridae-like morphology and polyhedral heads (approximately capsid 60–100 nm) with tail fibers. genome is ~38 kb in size, 66.0% GC content. contains 58 proposed open reading frames that are involved integration, DNA packaging, morphogenesis bacterial lysis. VB_CibM-P1...
A novel, aerobic, heterotrophic, orange-pigmented, Gram-staining-negative, rod-shaped, gliding bacterial strain, designated JLT2000(T), was isolated from surface water of the South China Sea. The strain oxidase- and catalase-positive. major cellular fatty acids JLT2000 T: were C12 : 0, iso-C15 1 G, iso-C17 0 3-OH, summed feature 3 (comprising C16 1ω7c and/or 1ω6c), C18 0. MK-7 respiratory quinone polar lipids phosphatidylcholine phosphatidylethanolamine. genomic DNA G+C content JLT2000(T)...
Paracoccus spp. are isolated from both terrestrial and aquatic habitats, indicating their ubiquitous existence in the environment. Here we present first phage this genus, vB_PmaS-R3, its complete genome sequence. vB_PmaS-R3 is a siphophage South China Sea. The sequence 42,093 bp, with G + C content of 56.36 %. Fifty-two open reading frames were predicted genome. can mainly be divided into three regions: genes for DNA metabolism, regulatory structure forming genes. Genes encoding metabolism...
Cyanate is a nitrogen and energy source for diverse marine microorganisms, playing important roles in the cycle. Despite extensive research on cyanate utilization, sources of this compound remain largely enigmatic. To unravel cyanate, distributions production during photochemical degradation natural dissolved organic matter (DOM) were investigated across various environments, including freshwater, estuarine, coastal areas Florida, continental slope regions North American mid-Atlantic Ocean...
Abstract SAR202 bacteria in the Chloroflexota phylum are abundant and widely distributed ocean. Their genome coding capacities indicate their potential roles degrading complex recalcitrant organic compounds However, our understanding of genomic diversity, vertical distribution, depth-related metabolisms is still limited by number assembled genomes. In this study, we apply deep metagenomic sequencing (180 Gb per sample) to investigate microbial communities collected from six representative...
Gene transfer agents (GTAs) are phage-like entities that package and random host genome fragments between prokaryotes. RcGTA, produced by
A Gram-stain-negative and facultatively anaerobic bacterial strain, designated GUOT, was isolated from surface water collected the South China Sea. Cells were non-flagellate, yellow, non-spore-forming rod-shaped. The 16S rRNA gene sequence comparisons with species in genus Arenibacter showed that strain GUOT shares highest similarity of 97.5 % echinorum palladensis. Average nucleotide identity digital DNA-DNA hybridization values between its related type strains 77.1-78.4% 20.8-26.2...
A novel Gram-stain-negative, aerobic, yellow-pigmented bacterium was isolated from seawater of Aoshan Bay, and designated as strain ASW18 T . Strain a long-rod-shaped without flagellum lacked gliding ability. Based on 16S rRNA gene phylogeny, showed the closest relationship to Croceivirga radicis MCCC 1A06690 , with sequence similarity 97.0 %. able grow at 25–40 °C, pH 5.5–9.5 0.5–9 % (w/v) NaCl. The genomic DNA G+C content 37.3 predominant cellular fatty acids were iso-C 15 : 0 17 3-OH 1 G....
Lytic and lysogenic infections are the main strategies used by viruses to interact with microbial hosts. The genetic information of prophages provides insights into nature phages their potential influences on Here, siphovirus vB_MoxS-R1 was induced from a Microbacterium strain isolated an estuarine Synechococcus culture. has high replication capability, estimated burst size 2000 virions per cell. represents novel phage genus-based genomic analysis. Six transcriptional regulator (TR) genes...
The bathypelagic zone is a large, dark, cold, high-pressure environment that undergoes extensive material and energy exchanges with the upper ocean deep biosphere. Viruses are important parts of ocean, transportation surface viruses has been considered as an source deep-sea viruses. However, limited information available about activity decay in ecosystem. Therefore, this study investigated stability representative viral isolates three morphological types different sizes infecting autotrophic...
Cyanophages play important roles in regulating the population dynamics, community structure, metabolism, and evolution of cyanobacteria aquatic ecosystems. Here, we report genomic analysis an estuarine cyanophage, S-CREM1, which represents a new genus T4-like cyanomyovirus exhibits genetic characteristics. S-CREM1 is lytic phage infects Synechococcus sp. CB0101. In contrast to many cyanomyoviruses that usually have broad host range, only infected original strain. addition cyanophage-featured...
Cyanophages affect the abundance, diversity, metabolism, and evolution of picocyanobacteria in marine ecosystems. Here we report an estuarine Synechococcus phage, S-CREM2, which represents a novel viral genus leads to establishment new T4-like cyanophage clade named cluster C. S-CREM2 possesses longest tail (~418 nm) among isolated cyanomyoviruses encodes six tail-related proteins that are exclusively homologous those predicted C cyanophages. Furthermore, may carry three regulatory virion,...
Synechococcus are picocyanobacteria with a cosmopolitan distribution. They capable of surviving in wide variety environmental conditions. have been isolated from the Chesapeake Bay during winter months, and they show an impressive tolerance to cold temperatures. Cold-adapted unique diverse, as complex phylogenetic lineages closely related subalpine cluster II, Bornholm Sea cluster, CB7 some novel which independent summer estuarine strains subcluster 5.2. CBW1002 CBW1006 first complete...