A5082548705- HIV Research and Treatment
- Immune Cell Function and Interaction
- Hepatitis C virus research
- T-cell and B-cell Immunology
- Liver Disease Diagnosis and Treatment
- HIV-related health complications and treatments
- Systemic Lupus Erythematosus Research
- Hepatitis B Virus Studies
- HIV/AIDS Research and Interventions
- Reproductive System and Pregnancy
- HIV/AIDS drug development and treatment
- Cytomegalovirus and herpesvirus research
- Viral Infections and Immunology Research
- Conflict, Peace, and Violence in Colombia
- Clinical Reasoning and Diagnostic Skills
- Social Sciences and Policies
- Ethics in Clinical Research
- Criminal Justice and Penology
- Sex work and related issues
- interferon and immune responses
- Monoclonal and Polyclonal Antibodies Research
- Innovations in Medical Education
- Cancer Immunotherapy and Biomarkers
- Extracellular vesicles in disease
- Atherosclerosis and Cardiovascular Diseases
Hospital Universitario Fundación Jiménez Díaz
2016-2025
Universidad Autónoma de Madrid
2016-2025
Hospital Universitario Rey Juan Carlos
2016-2025
Instituto de Salud Carlos III
2010-2013
Municipality of Medellín
2010
Hospital Clínico San Carlos
2010
Office of Infectious Diseases
2009
University of New Orleans
2004
Given that peginterferon-ribavirin treatment is poorly tolerated, there interest in the identification of predictors response, particularly HIV/hepatitis C virus (HCV)-coinfected patients respond less than HCV-monoinfected individuals. A single nucleotide polymorphism (SNP) near IL28B gene (rs12979860) has been shown to predict response carrying genotype 1. Information lacking for HIV/HCV-coinfected individuals and/or other HCV genotypes.From 650 patients, we identified those who had...
Introduction T-cell exhaustion has been involved in the pathogenesis of HIV infection. We have longitudinally analyzed PD1 and Tim3 surrogate markers T-cells exhaustion, parallel with other progression, its potential association CD4 changes treated untreated Patients methods 96 patients, 49 them followed absence cART (cART-naïve group) 47 after initiation (cART were included for a median 43 [IQR: 31–60] months. expression, CD8 activation, recent thymic emigrants, activation/apoptosis...
A single nucleotide polymorphism (SNP) upstream of the IL28B gene (rs12979860) predicts sustained virological response (SVR) to peginterferon-ribavirin therapy in chronic hepatitis C patients. There is scarce information regarding influence this SNP on early viral kinetics during therapy, particularly patients coinfected with HIV, whom treatment lower than virus (HCV)-monoinfected patients.We selected 196 HIV/HCV-coinfected individuals who had completed a course and validated outcome for...
Abstract HIV-specific T cells response and cell activation are frequently seen in exposed seronegative individuals (ESN). In this study, we report level of ESN partners HIV-infected patients presenting low or undetectable levels HIV-RNA. We evaluated 24 HIV-serodiscordant couples. were classified into three categories exposure to HIV (very low, moderate-high), considering HIV-RNA their infected partner frequency sexual high-risk practices within the last 12 mo. responses subsets by flow...
The immune system of people living with HIV (PLWH) is persistently exposed to antigens leading systemic inflammation despite combination antiretroviral treatment (cART). This inflammatory milieu promotes T-cell activation and exhaustion. Furthermore, it produces diminished effector functions including loss cytokine production, cytotoxicity, proliferation, disease progression. Exhausted T cells show overexpression checkpoint molecules (ICs) on the cell surface, programmed death protein 1...
Elite controllers (ECs) represent a unique subset of people living with HIV (PLWHs), who can suppress viral replication without requiring antiretroviral therapy (ART). However, despite this control, ECs exhibit increased incidences various comorbid conditions and heightened systemic inflammation, which has been linked to monocyte activation. In study, we performed an in-depth phenotypic analysis monocytes in cohort long-term (LTECs) compared them non-controller patients ART-mediated control...
The critical role of interleukin-28B (IL28B)/interferon-λ3 (IFN-λ3) polymorphisms on the susceptibility to hepatitis C virus infection and response peginterferon-ribavirin therapy has encouraged exploration similar effects other viruses. Given that IFN-λ mediates anti-HIV-1 activity, protective IL28B was examined in 29 seronegative individuals at risk for HIV-infection 68 HIV-positive carriers with without rapid progression immunodeficiency. No polymorphism found examining both HIV-disease...
The mechanism explaining the strong association between IL28B rs12979860 polymorphisms and treatment outcome in chronic hepatitis C remains unclear. We explore whether protein [interferon (IFN)-λ3] plasma levels may vary according to genotype and/or following pegylated IFN-α/ribavirin therapy. A total of 112 HIV/hepatitis virus (HCV)-coinfected patients who completed a course therapy were examined. Sustained virological response (SVR) was achieved by 56% patients. alleles genotyped using 5′...
T cell activation plays an important role in driving CD4 depletion during the course of HIV infection. There is scarce information about different subsets HIV(+) individuals experiencing distinct disease progression. The CD4(+) and CD8(+) its contribution to total were examined measuring CD38 expression by flow cytometry 120 HIV-infected 9 uninfected healthy controls. patients divided into four groups: 11 elite controllers (EC), 14 viremic (VC), 61 antiretroviral-naive typical progressors...
Background There are several contributors to HIV-pathogenesis or insufficient control of the infection. However, whether HIV/HCV-coinfected population exhibits worst evolution remains unclear. Recently, some markers immune exhaustion have been proposed as preferentially upregulated on T-cells during HIV-infection. Herein, we analyzed T-cell together with other that could be affected by HCV-coinfection. Patients and methods Ninety-six patients chronic HIV-infection (60 HIV-monoinfected 36...
Exosome-derived miR-21 was independently associated with CD4 T cell decline in HIV-1-infected elite controllers (OR 0.369, 95% CI 0.137-0.994, p = 0.049). Also, a negative correlation between expression and MCP-1 level found (r -0.649, 0.020), while no soluble biomarkers or cellular immune activation found.
Summary Due to the poor rate of response hepatitis C virus (HCV) with pegylated interferon and ribavirin treatment in HCV/HIV coinfected patients, key factors for predicting failure would be useful. We performed a retrospective study on 291 patients HCV treatment, who had early virological (EVR) data. IL28B IL28RA polymorphisms were using GoldenGate ® assay. Unfavourable genotypes at (rs12980275 AG/GG rs8099917 GT/GG) an unfavourable allele (rs10903035 G) associated failure. However, only...
Susceptibility to HIV transmission by sexual intercourse has been associated with cellular anti-HIV responses. We aimed also evaluate potential systemic humoral responses against in a group of HIV-exposed seronegative individuals (HESN) stable relationship HIV-infected partners.We recruited 27 serodiscordant couples. HESN were classified according exposure into very low/low and moderate/high risk. Plasma from partners tested for neutralizing capacity the recognition cell-surface expressed...
There is a lack of information about the stability these responses over time in subjects experiencing differences HIV disease progression. The functional profile Gag-specific and Nef-specific CD8T-cell based on simultaneous production macrophage inflammatory protein (MIP)-1beta, interleukin (IL)-2, tumor necrosis factor (TNF)-alpha was longitudinally assessed using flow cytometry 4 years 8 elite controllers (EC), viremic controllers, 10 antiretroviral-naive typical progressors, patients with...
Objectives The objective of this study was to seek correlates immune protection in HIV infection. We sought elucidate the association between presence human leucocyte antigen ( HLA ) alleles, as well killer immunoglobulin receptor KIR genotypes, and susceptibility infection a Spanish cohort ‐exposed seronegative HESN individuals. Methods A total 152 individuals were evaluated: 29 stable heterosexual relationships with an ‐infected partner admitting high‐risk sexual intercourse for at least...
Despite long-lasting HIV replication control, a significant proportion of elite controller (EC) patients may experience CD4 T-cell loss. Discovering perturbations in immunological parameters could help our understanding the mechanisms that be operating those experiencing loss control. A case–control study was performed to evaluate if alterations different homeostatic can predict ECs by comparing data from EC showing decline (cases) and stable counts (controls). The partial...
A recent study has pointed out to CD32a as a potential biomarker of HIV-persistent CD4 cells. We have characterized the level and phenotype CD32+ cells contained in different subsets T-cells its correlation with total HIV-DNA thirty HIV patients (10 typical progressors naïve for cART, 10 cART-suppressed patients, elite controllers). Total was quantified T-cells: Trm pTfh Level immunephenotype were analyzed these same by flow cytometry. CD32 expression similar groups, there no significant...
Background: Both viral and host factors influence response to peginterferon-α plus ribavirin (pegIFNα/RBV) in patients with chronic hepatitis C. The impact of these variables is more pronounced HIV/Hepatitis C virus (HCV)–coinfected individuals, whom treatment rates are lower. Methods: Virological responses at multiple time points were assessed all HIV/HCV-coinfected that completed a first course pegIFNα/RBV. Viral stratified by HCV geno/subtypes IL28B rs12979860 variants. Results: A total...
Objectives: To estimate the impact of interleukin 28B (IL28B) polymorphisms (rs12980275, rs8099917, rs7248668, and rs11881222) their haplotypes on hepatitis C virus (HCV) treatment (peg-interferon-α ribavirin) success in 324 HIV/HCV-coinfected patients. We also explore behavior plasma cytokine levels. Design: Retrospective follow-up study. Methods: Virologic response to HCV was measured by viral load at different endpoints: rapid virologic (RVR), early (EVR), end-of-treatment (ETVR)...
Cellular responses against hepatitis C virus (HCV) are impaired in HIV/HCV-coinfected patients showing uncontrolled viral replication and immune suppression. Very few studies have explored to what extent HCV-specific response improves as a consequence of control HIV by highly active antiretroviral therapy. We compared T-cell between patients, complete suppression, HCV-monoinfected patients.HCV-specific were examined 50 interferon-naive with chronic C: 27 HCV-mono-infected 23 on therapy...
Objective We investigated the association of genetic polymorphisms in chemokine and receptor genes with poor immunological recovery HIV patients starting combined antiretroviral therapy (cART) low CD4 T-cell counts. Methods A case-control study was conducted 412 HIV-infected cART count <200 cells/μL successful viral control for two years. increase below 200 after years on used to define INR (immunological non-responder) patients. Polymorphisms CXCL12, CCL5 CCR2 were genotyped using...