A5100621823- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- interferon and immune responses
- COVID-19 and Mental Health
- Infection Control and Ventilation
- Data-Driven Disease Surveillance
- Influenza Virus Research Studies
- Infection Control in Healthcare
- Asthma and respiratory diseases
- Long-Term Effects of COVID-19
- Viral gastroenteritis research and epidemiology
- Olfactory and Sensory Function Studies
- Sinusitis and nasal conditions
- COVID-19 epidemiological studies
- Biochemical Analysis and Sensing Techniques
- Helminth infection and control
- Parasitic Infections and Diagnostics
- Antibiotic Use and Resistance
- Healthcare professionals’ stress and burnout
- Technology Adoption and User Behaviour
- Zoonotic diseases and public health
- Environmental Chemistry and Analysis
- COVID-19 and healthcare impacts
University of Pennsylvania
2020-2024
Huazhong University of Science and Technology
2013-2024
Philadelphia VA Medical Center
2020-2024
Tongji Hospital
2013-2023
Land Consolidation and Rehabilitation Center
2023
Ministry of Natural Resources
2023
Central Hospital of Wuhan
2021-2022
Hebei North University
2020-2021
General Hospital of Central Theater Command
2020
Huazhong Agricultural University
2015-2020
At present, PCR-based nucleic acid detection cannot meet the demands for coronavirus infectious disease (COVID-19) diagnosis. Two hundred fourteen confirmed COVID-19 patients who were hospitalized in General Hospital of Central Theater Command People’s Liberation Army between 18 January and 26 February 2020 recruited. enzyme-linked immunosorbent assay (ELISA) kits based on recombinant severe acute respiratory syndrome 2 (SARS-CoV-2) nucleocapsid protein (rN) spike (rS) used detecting IgM IgG...
<h3>Importance</h3> Health care workers (HCWs) have high infection risk owing to treating patients with coronavirus disease 2019 (COVID-19). However, research on their and clinical characteristics is limited. <h3>Objectives</h3> To explore of HCWs COVID-19 discuss possible prevention measures. <h3>Design, Setting, Participants</h3> This single-center case series included 9684 in Tongji Hospital, Wuhan, China. Data were collected from January 1 February 9, 2020. <h3>Exposures</h3> Confirmed...
Coronaviruses are adept at evading host antiviral pathways induced by viral double-stranded RNA, including interferon (IFN) signaling, oligoadenylate synthetase–ribonuclease L (OAS-RNase L), and protein kinase R (PKR). While dysregulated or inadequate IFN responses have been associated with severe coronavirus infection, the extent to which recently emerged SARS-CoV-2 activates antagonizes these is relatively unknown. We found that infects patient-derived nasal epithelial cells, present...
Severe acute respiratory syndrome coronavirus (SARS-CoV)-2 has caused millions of deaths since its emergence in 2019. Innate immune antagonism by lethal CoVs such as SARS-CoV-2 is crucial for optimal replication and pathogenesis. The conserved nonstructural protein 15 (nsp15) endoribonuclease (EndoU) limits activation double-stranded (ds)RNA-induced pathways, including interferon (IFN) signaling, kinase R (PKR), oligoadenylate synthetase/ribonuclease L (OAS/RNase L) during diverse CoV...
ABSTRACT The SARS-CoV-2 pandemic was marked with emerging viral variants, some of which were designated as variants concern (VOCs) due to selection and rapid circulation in the human population. Here, we elucidate functional features each VOC linked variations replication rate. Patient-derived primary nasal cultures grown at air-liquid interface used model upper respiratory infection compared cell lines derived from lung epithelia. All VOCs replicated higher titers than ancestral virus,...
Abstract Background COVID-19 arise global attention since their first public reporting. Infection prevention and control (IPC) is critical to combat COVID-19, especially at the early stage of pandemic outbreak. This study aimed measure level healthcare workers’ (HCW’) self-reported IPC behaviors with risk emerges increases. Methods A cross-sectional was conducted in two tertiary hospitals. structured self-administered questionnaire delivered HCWs selected The dependent variables were...
Significance Middle East respiratory syndrome coronavirus (MERS-CoV) causes highly lethal disease. MERS-CoV encodes innate immune antagonists, including accessory proteins NS4a and NS4b, unique to the merbeco lineage of betacoronaviruses, nsp15 protein endoribonuclease (EndoU), conserved among all coronaviruses. While mutation each antagonist alone has little effect on immunity, infections with recombinant MERS-CoVs mutations EndoU in combination either or NS4b activate signaling pathways...
The nasal epithelium is the initial entry portal and primary barrier to infection by all human coronaviruses (HCoVs). We utilize epithelial cells grown at air-liquid interface, which recapitulate heterogeneous cellular population as well mucociliary clearance functions of in vivo epithelium, compare lethal [Severe acute respiratory syndrome (SARS)-CoV-2 Middle East syndrome-CoV (MERS-CoV)] seasonal (HCoV-NL63 HCoV-229E) HCoVs. All four HCoVs replicate productively cultures, though...
Background Outbreaks of hand-foot-mouth disease (HFMD) have been reported for many times in Asia during the last decades. This emerging has drawn worldwide attention and vigilance. Nowadays, prevention control HFMD become an imperative issue China. Early detection response will be helpful before it happening, using modern information technology epidemic. Method In this paper, a hybrid model combining seasonal auto-regressive integrated moving average (ARIMA) nonlinear neural network (NARNN)...
Abstract Background At present, PCR-based nucleic acid detection cannot meet the demands for coronavirus infectious disease (COVID-19) diagnosis. Methods 214 confirmed COVID-19 patients who were hospitalized in General Hospital of Central Theater Command People’s Liberation Army between January 18 and February 26, 2020, recruited. Two Enzyme-Linked Immunosorbent Assay (ELISA) kits based on recombinant SARS-CoV-2 nucleocapsid protein (rN) spike (rS) used detecting IgM IgG antibodies, their...
To assess the prevalence of burnout, secondary traumatic stress, and compassion satisfaction explore their impacts on self-reported hand hygiene among medical aid teams in COVID-19 period Wuhan, China.Cross-sectional study.A total 1,734 healthcare workers from 17 were surveyed. The survey included stress measured by professional quality life scale hygiene. Data collected between 5-7 March 2020. Multiple regression analyses performed.Burnout trauma at low average levels, was high levels....
The spread of resistance to carbapenems among Enterobacteriaceae has become a major public health problem in recent years. In this study, we describe an outbreak Klebsiella pneumoniae the neonatal ward. First, aimed study drug resistance, genetic relatedness, and transmission mechanism carbapenem-resistant K. pneumoniae; second, implemented infection control measures contain outbreak. We investigated 27 non-repetitive strains isolated from neonates five cultured around Polymerase chain...
SUMMARY Prolonged viral shedding is associated with severe status and poor prognosis of COVID-19 patients. Unexpectedly, here we report a non-severe patient the longest duration shedding. According to investigation on clinical epidemiological information this case, concluded that type virus might have low toxicity transmissibility, but prolonged infective ability was hardly be eliminated in body regular therapy. However, infusion plasma from recovered patients showed high efficiency...
Coronaviruses are adept at evading host antiviral pathways induced by viral double-stranded RNA, including interferon (IFN) signaling, oligoadenylate synthetase-ribonuclease L (OAS-RNase L), and protein kinase R (PKR). While dysregulated or inadequate IFN responses have been associated with severe coronavirus infection, the extent to which recently emerged SARS-CoV-2 activates antagonizes these is relatively unknown. We found that infects patient-derived nasal epithelial cells, present...
Abstract Background The outbreak of the recently emerged novel corona virus disease 2019 (COVID-19) poses a challenge for public health laboratories. We aimed to evaluate diagnostic value serological assay SARS-CoV-2. Methods A newly-developed ELISA IgM and IgG antibodies against N protein SARS-CoV-2 were used screen serums 238 admitted hospital patients with confirmed or suspected infection from February 6 14, 2020. RNA was detected by real time RT-PCR on pharyngeal swab specimens. Findings...
At the end of 2019 Wuhan witnessed an outbreak "atypical pneumonia" that later developed into a global pandemic. Metagenomic sequencing rapidly revealed causative agent this to be novel coronavirus denoted SARS-CoV-2. To provide snapshot pathogens in pneumonia-associated respiratory samples from prior emergence SARS-CoV-2, we collected bronchoalveolar lavage fluid 408 patients presenting with pneumonia and acute infections at Central Hospital between 2016 2017. Unbiased total RNA was...
Dear Editor, Coronavirus disease-19 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a threat to global health.1-3 The clinical spectrum in COVID-19 patients presents diversety.4, 5 Viral shedding is critical indicator for prognosis, and prolonged viral always predicts poor outcome.5, 6 In this report, however, we describe special case of family cluster with mild type COVID-19. one member, the duration from illness onset persisted over 49 days...
All respiratory viruses establish primary infections in the nasal epithelium, where efficient innate immune induction may prevent dissemination to lower airway and thus minimize pathogenesis. Human coronaviruses (HCoVs) cause a range of pathologies, but host viral determinants disease during common cold versus lethal HCoV are poorly understood. We model initial site infection using epithelial cells cultured at an air-liquid interface (ALI). HCoV-229E, HCoV-NL63, human rhinovirus-16...