A5068812467- Autoimmune Bullous Skin Diseases
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Urticaria and Related Conditions
- Platelet Disorders and Treatments
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Drug-Induced Adverse Reactions
- vaccines and immunoinformatics approaches
- Eosinophilic Disorders and Syndromes
- Immune Response and Inflammation
- Pregnancy and preeclampsia studies
- Antimicrobial Peptides and Activities
- Toxin Mechanisms and Immunotoxins
- Genetics, Bioinformatics, and Biomedical Research
- S100 Proteins and Annexins
- CAR-T cell therapy research
- Medicine, History, and Philosophy
- Galectins and Cancer Biology
- Dermatology and Skin Diseases
- Immunodeficiency and Autoimmune Disorders
- Barrier Structure and Function Studies
- Systemic Lupus Erythematosus Research
- Glycosylation and Glycoproteins Research
Normandie Université
2014-2025
Inserm
2016-2025
Université de Rouen Normandie
2016-2025
Centre Hospitalier Universitaire de Rouen
2016-2023
Physiopathologie et Epidémiologie des Maladies Respiratoires
2018-2022
Physiopathologie, autoimmunité, maladies neuromusculaire et thérapie régénératrice
2018-2021
Hôpital Charles-Nicolle
2010-2017
Institute for Research and Innovation in Biomedicine
2013-2017
Agency for Science, Technology and Research
2010-2014
Singapore Immunology Network
2010-2014
Changes in the B cell repertoire mediate long-lasting therapeutic effects of rituximab pemphigus patients.
Abstract IL‐10‐producing regulatory B cells have been identified in mice and shown to downregulate inflammation, making them potentially important for maintenance of tolerance. In this study, we isolated from human blood spleen, showed that after a short period ex vivo stimulation number these produced IL‐10. The did not fall within single clearly defined subpopulation, but were enriched both the memory (CD27 + ) transitional (CD38 high B‐cell compartments. Combined CpG‐B+anti‐Ig was most...
Abstract Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by pathogenic autoantibodies directed against nuclear Ags and immune complex deposits in damaged organs. Environmental factors have been thought to play a role the onset of disease. The recognition these mediated TLRs, particular TLR2 TLR4 which bind pathogen-associated molecular patterns Gram+ Gram− bacteria, respectively. We attempted determine TLRs SLE creating TLR2- or TLR4-deficient C57BL/6lpr/lpr mice....
CD11c+ B cells have been reported to be increased in autoimmune diseases, but they are detected the blood of healthy individuals as well. We aimed characterize from donors by flow cytometry, microarray analysis and vitro functional assays. Here we report that a distinct subpopulation cells, enriched memory sub-population even if their phenotype is heterogeneous, with overexpression genes involved cell activation differentiation well antigen presentation. Upon activation, can differentiate...
Importance The Ritux 3 trial demonstrated the short-term efficacy and safety of first-line treatment with rituximab compared a standard corticosteroid regimen in pemphigus. No data on long-term follow-up patients who received as first line are available. Objective To assess regimen. Design, Setting, Participants This 7-year study included pemphigus from 25 dermatology departments France January 1, 2010, to December 31, 2015. Exposure Patients were initially randomized plus prednisone group...
Corticosteroids (CS) with or without adjuvant immunosuppressant agents are standard treatment for pemphigus vulgaris (PV). The efficacy of therapies in minimizing steroid‐related adverse events (AEs) is unproven. To utilize data collected a French investigator‐initiated, phase III, open‐label, randomized controlled trial to demonstrate the and safety rituximab seek approval its use PV. This was an independently conducted post hoc analysis moderate‐to‐severe PV subset enrolled Ritux 3 study....
Rituximab and short-term corticosteroid therapy are the criterion standard treatments for patients with newly diagnosed moderate to severe pemphigus.To examine factors associated relapse in pemphigus treated rituximab.This post hoc analysis of a randomized clinical trial (Comparison Between Treatment Oral Corticosteroid Patients With Pemphigus [RITUX 3]) conducted from January 1, 2010, December 31, 2015, included 20 dermatology departments tertiary care centers France RITUX 3 according...
Murine gut intraepithelial (IEL) T cell receptor (TCR)-α/β1 lymphocytes bearing CD8α/β or CD8α/α coreceptors have been shown previously to express different oligoclonal TCR β chain repertoires in the same mouse, agreement with other evidence indicating that these two populations belong ontogenic lineages, only CD8α/β1 IELs being fully thymus dependent. CD8α/β1, but not CD8α/α1, are also present lamina propria. Here, we show CD8α/β+ from propria and epithelium both oligoclonal, they share...
Immunoglobulin G (IgG) levels are maintained by the IgG-recycling neonatal Fc-receptor (FcRn). Pemphigus vulgaris and pemphigus foliaceus debilitating autoimmune disorders triggered IgG autoantibodies against mucosal epidermal desmogleins. Recently, a phase 2 clinical trial (NCT03334058; https://clinicaltrials.gov/NCT03334058) was completed in participants with using efgartigimod, an FcRn inhibitor, combination prednisone. Efgartigimod demonstrated early effect on diease activity well...
Abstract Regulatory B cells (B-reg) produce IL-10 and suppress inflammation in both mice humans, but limited data on the phenotype function of these have precluded detailed assessment their contribution to host immunity. In this article, we report that human B-reg cannot be defined based a composed conventional cell markers, production can elicited CD27+ memory population naive subset after only brief stimulation vitro. We therefore sought obtain better definition IL-10–producing B-regs...
Desmocollin-3 (DSC3) is a calcium-dependent desmosomal cadherin that plays an essential role in cell-cell adhesion. IgG antibodies (Abs) directed against the extracellular (EC) domain of DSC3 have occasionally been detected rare types pemphigus. Investigations into prevalence anti-EC-DSC3 Abs and those targeting intracellular (IC) pemphigus vulgaris foliaceus sera, their potential pathogenic activity, yielded conflicting results. To assess pathogenicity EC IC domains patients with Anti-DSC3...
This cohort study examines the safety and efficacy of rituximab for patients with pempigus foliaceus.
Bullous Pemphigoid is the most common auto-immune bullous skin disease. It characterized by production of auto-antibodies directed against 2 proteins hemi-desmosome (BP180 and BP230). We assessed efficacy mechanisms action rituximab, an anti-CD20 monoclonal antibody, in 17 patients with severe relapsing type pemphigoid. The phenotype, cytokine gene expression, rearrangement BP180-specific B-cell receptor genes were performed over years following treatment. At end study, 5 had died, 3...
Pemphigus Vulgaris is an autoimmune disease of the skin and mucous membranes, which due to production pathogenic autoantibodies targeting desmoglein (DSG) 1 3, are adhesion proteins keratinocytes. Rituximab anti-CD20 mAb induces a prolonged depletion blood B cells. We recently showed that rituximab was more effective than standard oral corticosteroid (CS) treatment, allowing 90% patients achieve complete remission (CR). Additionally, we DSG-specific-B (DSG positive) cells were still...
Reduced peptide bond pseudopeptide analogues have been examined for their ability to bind murine class I molecules of the major histocompatibility complex (MHC). Eight an antigenic derived from Plasmodium berghei (H-Ser252-Tyr-Ile-Pro-Ser-Ala-Glu-Lys-Ile260-OH) were obtained by systematically replacing one at a time reduced ψ(CH2-NH). The resulting then tested binding recombinant single chain SC-Kd molecule. comparative results show that five can efficiently mimic parent while introduction...
Few data are available on the involvement of brain microvascular endothelial cells (BMECs) in excitotoxic neonatal lesions. Therefore, we developed an original approach for investigating mouse-derived BMECs vitro. We hypothesized that newborn and adult BMEC cultures would show age-related differences phenotype sensitivity to glutamate. Expression monocarboxylate transporter, MCT1, was higher than BMECs, whereas expression glucose GLUT1, overexpressed N-methyl-D-aspartate receptor NR1 subunit...
Abstract Background Pemphigus vulgaris (PV) is characterized by autoantibodies targeting keratinocyte adhesion proteins desmoglein (Dsg) 1 and 3, the human leukocyte antigen (HLA) predisposition allele HLA-DRB1*0402. Treatment using rituximab (RTX) combined with short-term corticosteroids (CS) allows disease control long-lasting remission. Objectives The principal aim of this study was to evaluate impact RTX on circulating subpopulations Dsg3-specific T lymphocytes that specifically regulate...
Abstract Skeletal muscles account for more than 30% of the human body, yet mechanisms immunological tolerance to this tissue remain mainly unexplored. To investigate muscle-specific proteins, we generated transgenic mice expressing neo-autoantigen OVA exclusively in skeletal muscle (SM-OVA mice). SM-OVA were bred with OT-I or OT-II that possess a TCR specific peptides presented by MHC class I II, respectively. Tolerance did not involve clonal deletion, anergy an increased regulatory T cell...
Abstract T cell development is regulated at two critical checkpoints that involve signaling events through the TCR. These signals are propagated by kinases of Src and Syk families, which activate several adaptor molecules to trigger Ca2+ release and, in turn, Ca2+/calmodulin-dependent protein kinase II (CaMKII) activation. In this study, we show a constitutively active form CaMKII antagonizes TCR impairs positive selection thymocytes mice. Following engagement, decreases TCR-mediated CD3ζ...
Pemphigus is a life-threatening auto-immune blistering disease of the skin and mucous membrane that caused by production auto-antibodies (auto-Abs) directed against adhesion proteins: desmoglein 1 3. We demonstrated in "Ritux3" trial, high efficacy rituximab, an anti-CD20 recombinant monoclonal antibody, as first-line treatment for pemphigus. However, 25% patients relapsed during six-month period after rituximab treatment. These early relapses were associated with lower decrease...