A5044631143- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Neuroblastoma Research and Treatments
- Advanced Biosensing Techniques and Applications
- Hippo pathway signaling and YAP/TAZ
- Pancreatic and Hepatic Oncology Research
- Immune Cell Function and Interaction
- Lipid Membrane Structure and Behavior
- Caveolin-1 and cellular processes
- Immune cells in cancer
- Sphingolipid Metabolism and Signaling
- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Immune Response and Inflammation
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Bladder and Urothelial Cancer Treatments
- Wnt/β-catenin signaling in development and cancer
- RNA modifications and cancer
- HER2/EGFR in Cancer Research
- Chromosomal and Genetic Variations
- Cancer-related Molecular Pathways
European Institute of Oncology
2021-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2024
University of Cambridge
2019-2020
Chromosome instability (CIN) is the most common form of genome and a hallmark cancer. CIN invariably leads to aneuploidy, state karyotype imbalance. Here, we show that aneuploidy can also trigger CIN. We found aneuploid cells experience DNA replication stress in their first S-phase precipitate continuous This generates repertoire genetically diverse with structural chromosomal abnormalities either continue proliferating or stop dividing. Cycling display lower complexity compared arrested...
CD8+ T cells are a major prognostic determinant in solid tumors, including colorectal cancer (CRC). However, understanding how the interplay between different immune impacts on clinical outcome is still its infancy. Here, we describe that interaction of tumor infiltrating neutrophils expressing high levels CD15 with effector memory (TEM) correlates progression. Mechanistically, stromal cell-derived factor-1 (CXCL12/SDF-1) promotes retention within increasing crosstalk cells. As consequence...
Mutations in ADNP (Activity-Dependent Neuroprotective Protein) are among the most frequent monogenic causes of autism spectrum disorder (ASD) and lead to Helsmoortel-Van der Aa syndrome (HVDAS). Yet how dysfunction leads HVDAS is unclear. We employed patient-derived induced pluripotent stem cells, cortical organoids KO human neural cells (hNSCs) clarify cellular molecular mechanism onset. purified an ADNP-KDM1A-GTF2I (AKG) protein complex from hNSCs show that it targets transposable elements...
Abstract Replicative stress (RS) is emerging as a promising therapeutic target in oncology, yet full exploitation of its potential requires detailed understanding the mechanisms and genes involved. Here, we investigated RNA helicase Senataxin (SETX), an enzyme that resolves RNA-DNA hybrids R-loops, to address role preventing RS by oncogenic Myc. Upon Myc activation, silencing SETX led selective engagement DNA damage response (DDR) robust cytotoxicity. Pharmacological dissection upstream...
Germline mutations of YY1 cause Gabriele-de Vries syndrome (GADEVS), a neurodevelopmental disorder featuring intellectual disability and wide range systemic manifestations. To dissect the cellular molecular mechanisms underlying GADEVS, we combined large-scale imaging, single-cell multiomics gene regulatory network reconstruction in 2D 3D patient-derived physiopathologically relevant cell lineages. haploinsufficiency causes pervasive alteration type specific transcriptional networks,...
Signalling is of particular importance in immune cells, and upstream the signalling pathway many membrane receptors are functional only as complexes, co-locating with lipid species. Work over last 15 years has shown that plasma composition close to a critical point phase separation, evidence cells adapt their ways alter proximity this thermodynamic point. Macrophage key component innate system, responsive infections regulate local state inflammation. We investigate changes membrane’s...
Understanding the cell cycle at single-cell level is crucial for cellular biology and cancer research. While current methods using fluorescent markers have improved study of adherent cells, non-adherent cells remain challenging. In this study, we addressed gap by combining a specialized surface to enhance attachment, FUCCI(CA)2 sensor, an automated image analysis pipeline, custom machine learning algorithm. This approach enabled precise measurement phase durations in cells. method was...
Advances in bladder cancer (BCa) treatment have been hampered by the lack of predictive biomarkers and targeted therapies. Here, we demonstrate that loss tumor suppressor NUMB promotes aggressive tumorigenesis worsens disease outcomes. Retrospective cohort studies show NUMB-loss correlates with poor prognosis post-cystectomy muscle-invasive BCa patients increased risk muscle invasion progression non-muscle invasive patients. In mouse models, Numb ablation induces spontaneous sensitizes...
Germline mutations of YY1 cause Gabriele-de Vries syndrome (GADEVS), a neurodevelopmental disorder featuring intellectual disability and wide range systemic manifestations. To dissect the cellular molecular mechanisms underlying GADEVS, we combined large-scale imaging, single-cell multiomics gene regulatory network reconstruction in 2D 3D patient-derived physiopathologically relevant cell lineages. haploinsufficiency causes pervasive alteration type specific transcriptional networks,...
Abstract Chromosome instability (CIN) is the most common form of genome and a hallmark cancer. CIN invariably leads to aneuploidy, state karyotype imbalance. Here, we show that aneuploidy can also trigger CIN. We found aneuploid cells experience DNA replication stress in their first S-phase precipitate continuous This generates repertoire genetically diverse either continue proliferating or stop dividing. Cycling display lower complexity compared arrested ones increased expression repair...
Understanding the details of cell cycle at level individual cells is critical for both cellular biology and cancer research. While existing methods using specific fluorescent markers have advanced our ability to study in that adhere surfaces, there a clear gap when it comes non-adherent cells. In this study, we combine specialized surface improve attachment, genetically-encoded FUCCI(CA)2 sensor, an automated image processing analysis pipeline, custom machine-learning algorithm. This...
ABSTRACT Bladder cancer (BCa) is one of the most challenging and costly cancers to treat, yet little progress has been made on development predictive biomarkers targeted therapies. Here, we uncover a critical function Numb as tumor suppressor in bladder, identifying loss expression causal alteration BCa that underlies biological aggressiveness disease progression. Through retrospective cohort studies, established Numb-deficient status correlates with worse overall survival post-cystectomy...
Understanding the details of cell cycle at level individual cells is critical for both cellular biology and cancer research. While existing methods using specific fluorescent markers have advanced our ability to study in that adhere surfaces, there a clear gap when it comes non-adherent cells. In this study, we combine specialized surface improve attachment, genetically-encoded FUCCI(CA)2 sensor, an automated image processing analysis pipeline, custom machine-learning algorithm. This...
Understanding the cell cycle at single-cell level is crucial for cellular biology and cancer research. While current methods using fluorescent markers have improved study of adherent cells, non-adherent cells remain challenging. In this study, we addressed gap by combining a specialized surface to enhance attachment, FUCCI(CA)2 sensor, an automated image analysis pipeline, custom machine learning algorithm. This approach enabled precise measurement phase durations in cells. method was...
Abstract The gene neurofibromatosis 1 (NF1) is increasingly recognized as a key somatic driver of cancerogenesis, in addition to its well-known role the germline determinant onco-developmental syndrome Neurofibromatosis. NF1 best characterized negative regulator RAS activation, but several lines evidence suggest that it may have additional, poorly functions. In breast cancer, loss known be associated with resistance endocrine and HER2-targeted therapy. Here, using HER2+ cancer cell...
Abstract Background: Despite major technological and conceptual advancements, treatment decisions in HER2+ metastatic breast cancer (mBC) remain largely based on clinical evidence, with no established predictive biomarkers to direct for individual patients. The tumour suppressor Neurofibromatosis 1 (NF1) has been implicated endocrine resistance but its role remains incompletely characterized mBC. NF1 is best known as a GTPase-activating protein (GAP) that attenuates RAS signalling. However,...
Abstract Signalling is of particular importance in immune cells, and upstream the signalling pathway many membrane receptors are functional only as complexes, co-locating with lipid species. Work over last 15 years has shown that plasma composition close to a critical point phase separation, evidence cells adapt their ways alter proximity this thermodynamical point. Macrophage key component innate system, responsive infections, regulating local state inflammation. We investigate changes...