A5065254255- Hepatitis B Virus Studies
- Hepatitis C virus research
- Single-cell and spatial transcriptomics
- Immune cells in cancer
- Bacteriophages and microbial interactions
- Viral Infections and Immunology Research
- Atherosclerosis and Cardiovascular Diseases
- Protein purification and stability
- HIV Research and Treatment
- Plant Virus Research Studies
Brigham and Women's Hospital
2023
Harvard University
2023
Wellcome Trust
1994-1995
University of Reading
1989
Interferon-γ (IFNγ) signaling plays a complex role in atherogenesis. IFNγ stimulation of macrophages permits vitro exploration proinflammatory mechanisms and the development novel immune therapies. We hypothesized that study macrophage subpopulations could lead to anti-inflammatory interventions.
The protease activity of the hepatitis C virus (HCV) NS3 protein has been investigated using transient expression methods in mammalian cells, as well vitro transcription/translation systems. We confirmed that NS3-5 polyprotein rabbit reticulocyte lysates results efficient cis processing at NS3/NS4 junction. However, other predicted sites NS3-mediated cleavage varied markedly efficiency, site most susceptible being between NS5A and NS5B. Time-course analysis proteolytic HCV non-structural...
The non-structural protein NS3 of hepatitis C virus has been expressed in bacteria as a polyhistidine fusion which can be produced soluble form and easily purified by affinity chromatography. Using an vitro transcription translation system we have able to demonstrate that this proteolytically process substrate molecules derived from the region polyprotein. assay optimize basic biochemical characteristics enzyme. Parallel experiments show full-length also possesses ATPase activity, indicating...
A cassette vector has been constructed which allows the rapid and extensive modification of one neutralizing antigenic sites Sabin 1 poliovirus vaccine strain, P1/LSc 2ab. Unique restriction endonuclease flanking site have engineered into a full-length infectious cDNA clone with minimal alteration to coding sequence. This facilitates replacement this region by oligonucleotides encoding foreign amino acid sequences. Our results indicate that is highly flexible in terms number sequence acids...