A5103015294- Epilepsy research and treatment
- Neuroscience and Neuropharmacology Research
- Pharmacological Effects and Toxicity Studies
- Ion channel regulation and function
- Genetics and Neurodevelopmental Disorders
- Metabolism and Genetic Disorders
- Genomics and Rare Diseases
- Mitochondrial Function and Pathology
- Single-cell and spatial transcriptomics
- Ion Transport and Channel Regulation
- Immune cells in cancer
- Neonatal and fetal brain pathology
- Autophagy in Disease and Therapy
- Bioactive Natural Diterpenoids Research
- Machine Learning in Bioinformatics
- Phagocytosis and Immune Regulation
- MicroRNA in disease regulation
- Synthetic Organic Chemistry Methods
- Epigenetics and DNA Methylation
- Cell death mechanisms and regulation
- Gut microbiota and health
- RNA modifications and cancer
- EEG and Brain-Computer Interfaces
- Immune Cell Function and Interaction
- Folate and B Vitamins Research
The Seventh Affiliated Hospital of Sun Yat-sen University
2020-2025
Sun Yat-sen University
2020-2025
Zhengzhou University
2016-2024
Ministry of Education of the People's Republic of China
2020
ABSTRACT Next generation sequencing (NGS) technology has made significant progress in the genetic diagnosis and treatment of epilepsy. However, studies on epilepsy with different etiologies remain relatively limited. In this study, whole‐genome or whole‐exome was performed 158 unrelated patients various etiologies, identified variants were analyzed for their association 1356 seizure‐related genes database. Additionally, pathogenicity likely those associated known evaluated. The results...
Oridonin, a diterpenoid compound isolated from traditional Chinese medicine Rabdosia rubescens, has shown antitumor effects to esophageal cancer. However, its molecular mechanism is not fully understood, which limits clinical application. In the present study, we used RNA-seq analysis check transcriptome changes after oridonin treatment and found genes controlling GSH-ROS system were up-regulated, namely SLC7A11, TXNRD1, TRIM16, SRXN1, GCLM, GCLC. Furthermore, our data suggest that...
Abstract Changes in the expression of HCN ion channels leading to changes I h function and neuronal excitability are considered be possible mechanisms involved epileptogenesis kinds human epilepsy. In previous animal studies febrile seizures temporal lobe epilepsy, HCN1 HCN2 at different time points parts brain were not consistent, suggesting that transcriptional disorders involving HCNs play a crucial role epileptogenic process. Therefore, we aimed assess regulation Medial epilepsy with...
Objective: It aimed to construct the miRNA-mRNA regulatory network related ion channel genes in mesial temporal lobe epilepsy (mTLE), and further identify vital node network. Methods: Firstly, we identified channel-related differentially expressed (DEGs) mTLE using IUPHAR/BPS Guide Pharmacology (GTP) database, neXtProt GeneCards high-throughput sequencing dataset. Then STRING online database was used a protein-protein interaction (PPI) of DEGs, hub module PPI cytoHubba plug-in Cytoscape...
The gut microbiota and the microbiota-gut-brain axis have gained considerable attention in recent years, emerging as key players mechanisms that mediate occurrence progression of many central nervous system-related diseases, including epilepsy. In clinical practice, one side effects quinolone antibiotics is a lower seizure threshold or aggravation. However, underlying mechanism remains unclear. We aimed to unravel intrinsic through 16S rRNA sequencing serum untargeted metabolomic analysis...
Aims: To investigate the effects of single nucleotide polymorphisms (SNPs) in genes one-carbon metabolism (OCM) related enzymes and anti-epileptic drug (AED) monotherapy on homocysteine (Hcy) patients with epilepsy, to further explore specific SNPs that may increase patients' susceptibility AEDs Hcy imbalance. Method: This case-control study analyzed 279 including receiving valproate (VPA) ( n = 53), oxcarbazepine (OXC) 71), lamotrigine (LTG) 55), or levetiracetam (LEV) 35) who had not taken...
With detailed studies of ATP1A3-related diseases, the phenotypic spectrum ATP1A3 has greatly expanded. This study aimed to potentially identify mechanisms by which caused neurological dysfunction analyzing clinical features and phenotypes exploring distribution patterns mutations in subregions protein, thus providing new effective therapeutic approaches.Databases PubMed, Online Mendelian Inheritance Man, Human Gene Mutation Database, Wanfang Data, Embase were searched for case reports...
We aim to determine whether automatically detected ripple rate (ADRR) of 10-min scalp electroencephalography (EEG) during slow-wave sleep can be a useful tool for rapid epilepsy differentiation and seizure activity assessment, we analyze the clinical factors that may affect rates.We retrospectively included 336 patients who underwent long-term video-EEG with sampling ≥1000 Hz, three groups were established based on their final diagnosis (non-epilepsy; non-active [epilepsy being seizure-free...
Abstract Background To investigate the effect of long-term febrile convulsions on gene expression in mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS) and explore molecular mechanism MTLE-HS. Methods Microarray data MTLE-HS were obtained from Gene Expression Omnibus database. Differentially expressed genes (DEGs) between without seizure history screened by GEO2R software. Pathway enrichment ontology DEGs analyzed using DAVID online database FunRich Protein–protein...
<title>Abstract</title> Background Temporal lobe epilepsy (TLE) is a prevalent type of focal refractory with uncertain underlying molecular mechanisms. This study aimed to elucidate the hub genes and potential mechanisms associated TLE in human brain, as well characterize immune infiltration landscape. The datasets GSE190451 GSE205661 were retrieved from Gene Expression Omnibus (GEO) database for differential expression analysis weighted gene co-expression network (WGCNA). Subsequent...
Abstract Background This study was designed to characterize human PRRT2 gene and protein, in order provide theoretical reference for research on regulation of expression its involvement the pathogenesis paroxysmal kinesigenic dyskinesia other related diseases. Method Biological softwares Protparam, Protscale, MHMM, SignalP 5.0, NetPhos 3.1, Swiss-Model, Promoter 2.0, AliBaba2.1 EMBOSS were used analyze sequence characteristics, transcription factors their binding sites promoter region gene,...
A versatile intermediate γ-butyrolactone 7 was prepared from L-alanine with 96% ee value, which could be used as a common for the preparation of side-chain acid homoharringtonine and harringtonine in 26.6% 27% overall yield respectively.The key steps involved [2, 3]-Meisenheimer rearrangement, allylic bromination, Barbier reaction.Some interesting experimental phenomena effective solutions to these issues are reported herein.