A5101957241- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Alzheimer's disease research and treatments
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Heavy Metal Exposure and Toxicity
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- vaccines and immunoinformatics approaches
- Pneumonia and Respiratory Infections
- Heavy metals in environment
- Microbial infections and disease research
- Virology and Viral Diseases
- Parvovirus B19 Infection Studies
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Cholinesterase and Neurodegenerative Diseases
- Viral Infections and Vectors
- Tryptophan and brain disorders
- Plant tissue culture and regeneration
- Bioinformatics and Genomic Networks
- RNA Research and Splicing
- Computational Drug Discovery Methods
- Neonatal Respiratory Health Research
- SARS-CoV-2 detection and testing
Beijing Jiaotong University
2015-2024
National Institute for Viral Disease Control and Prevention
2013
XinHua Hospital
1988
Abstract Human respiratory syncytial viruses (RSVs) are classified into two major groups (A and B) based on antigenic differences in the G glycoprotein. To investigate circulating characteristics phylodynamic history of RSV, we analyzed genetic variability evolutionary pattern RSVs from 1977 to 2019 this study. The results revealed that there was no recombination event intergroup. Single nucleotide polymorphisms (SNPs) were observed through genome with highest occurrence rate gene. Five six...
Amyloid-β peptide (Aβ) is recognized by many as the leading cause of Alzheimer's disease (AD), and Aβ oligomers play a major role in early-onset form AD. Recently, application passive immunization targeting has been investigated
Human respiratory syncytial virus (RSV) is the most significant cause of acute lower infection in children. However, there no licensed vaccine available. Here, we investigated effect five or 20 copies C-Class CpG ODN (CpG-C) motif incorporated into a plasmid DNA encoding RSV fusion (F) glycoprotein on vaccine-induced immune response. The addition CpG-C enhanced serum binding and virus-neutralizing antibody responses BALB/c mice immunized with vaccines. Moreover, vaccinated CpG-modified...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants have been emerging and circulating globally since the start of COVID-19 pandemic, which B.1.617 lineage that was first reported in India at end 2020, soon became predominant. Tracing genomic variations understanding their impact on viral properties are foundations for vaccine drug development mitigation measures to be taken or lifted. In this study, 1,051 near-complete genomes 1,559 spike (S) sequences belonging were...
Amyloid β-protein (Aβ) has been causally implicated in the neurodegenerative processes that accompany Alzheimer's disease. Soluble oligomers of Aβ1-42 fragment are thought to be significantly more neurotoxic than higher molecular weight aggregates. We report isolation and characterization a mouse monoclonal antibody (MAb) directed against soluble oligomers. Synthetic were assembled vitro; these used immunize mice, hybridomas isolated following myeloma fusion splenocytes from immunized...
This paper presents a method for establishing the relationship between zeta potential and movement of polyelectrolyte through solid-state nanopore by employing electrostatic-hydrodynamic theory. The influence salt concentration, surface charge density, potential, them are discussed. For selected driven an electric field, dependence on velocity translocation is shown, which demonstrates that mediated predicts modification can become effective means managing regulating translocation.
Abstract Background Human respiratory syncytial virus (RSV) is a serious pediatric pathogen of the lower tract. Currently, there no clinically approved vaccine against RSV infection. Recent studies have shown that helper-dependent adenoviral (HDAd) vectors may represent effective and safe vectors. However, viral challenge has not been investigated following mucosal vaccination with HDAd vector vaccines. Methods To explore role played by as an intranasally administered vector, we constructed...
Abstract Fluorescence‐based methods are promising for measuring amyloid beta (Aβ) oligomers, given their capacity to analyse a sample at the single‐molecule level. As attachment of fluorescent labels may influence biochemical properties Aβ effects on oligomers must be evaluated. In this paper, we compared impacts five different dyes aggregation 42 using fluorescence correlation spectroscopy (FCS). We found that BODIPY® FL‐C5 (BP), N‐hydroxysuccinimide rhodamine B ester (RB) and...
Although the exact etiology and pathogenesis of Alzheimer's disease (AD) are still unclear, amyloid‐β (Aβ) generated by proteolytic processing precursor protein (APP) aggregate to form toxic amyloid species. Kinesin‐1 is first identified ATP‐dependent axonal transport motor that has been proven affect Aβ generation deposition. In this paper, we applied dual‐color fluorescence cross‐correlation spectroscopy (DC‐FCCS) investigate direct interaction with kinesin‐1 at single‐molecule level in...
Excess cadmium (Cd) intake poses a general risk to health and the kidneys in particular. Among indices of renal dysfunction under Cd burden measures are urinary N-acetyl-β-D-glucosidase (UNAG) β2-microglobulin (Uβ2-MG) enzymes. However, end-pointed values threshold remain controversial because scopes fluctuate widely.To ascertain clinical benchmark dose (UCd) for dysfunction, 1595 residents near site were surveyed. Urine was sampled assayed. A low (BMDL) obtained by fitting UCd levels index...
It has been argued that vaccine-breakthrough infections of SARS-CoV-2 would likely accelerate the emergence novel variants with immune evasion. This study explored evolutionary patterns Delta variant in countries/regions relatively high and low vaccine coverage based on large-scale sequences. Our results showed (i) sequences were grouped into two clusters (L R); R cluster was dominant, its proportion increased over time higher high-vaccine-coverage areas; (ii) genetic diversities than those...
Alzheimer’s disease (AD) is the most common form of age-related dementia, and urgent problem that it currently incurable. Amyloid-β (Aβ) peptide believed to play a major role in pathogenesis AD. We previously reported an Aβ N-terminal amino acid targeting monoclonal antibody (MAb), A8, inhibits fibril formation has potential as immunotherapy for AD based on mouse model. To further study underlying mechanisms, we tested our hypothesis single chain fragment variable (scFv) without Fc capable...
Human respiratory syncytial virus (RSV) is the single most important cause of lower tract disease in infants and young children a major viral agent responsible for immunosuppressed individuals elderly, but no vaccines antiviral drugs are available. Herein recombinant RSV (rRSV) encoding enhanced green fluorescence protein (EGFP, rRSV-EGFP) was constructed potential screening anti-RSV investigated. The plasmid pBRATm-rRSV-EGFP, containing T7 transcription cassette composed promoter,...
Human respiratory syncytial virus (RSV) is a ubiquitous pediatric pathogen causing serious lower tract disease worldwide. No licensed vaccine currently available. In this work, the coding gene for mDS-Dav1, full-length and prefusion conformation RSV fusion glycoprotein (F), was designed by introducing stabilized F (preF) mutations from DS-Cav1 into encoding of wild-type (wtRSV) protein. The recombinant adenovirus mDS-Cav1, rChAd63-mDS-Cav1, constructed based on serotype 63 chimpanzee vector...
Abstract Extracellular plaque deposits of β‐amyloid peptide (Aβ) are one the main pathological features Alzheimer's disease (AD). The aggregation Aβ 42 species, especially oligomers, is still an active research field in AD pathogenesis. Secretory clusterin protein (sCLU), extracellular chaperone, plays important role Although sCLU interacts directly with vitro and vivo, mechanism not clear. In this paper, His‐tagged (sCLU‐His) was cloned, expressed purified, we applied florescence resonance...